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  • 1
    Electronic Resource
    Electronic Resource
    THE CEREBELLAR GLOMERULUS: ISOLATION AND METABOLIC PROPERTIES OF A PURIFIED FRACTION (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 27 (1976), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— A method is described for large scale isolation of glomerular complexes from rabbit cerebellum. The purity of the fraction is 90–95%, measured by quantitative electron microscopy. In addition biochemical markers indicate a high degree of particle integrity. The glomeruli occur as mechanically separable units at 20–40 days of animal age and the amount of protein per particle is 15–20 × 10−11 g. The glomeruli accumulate [3H]GABA, and exhibit both high (Km 15μM) and low (Km 0.5 mM) affinity uptake properties. Glomeruli oxidize α-glycerophosphate and succinate particularly well, while glutamate, pyruvate and α-ketoglutarate are less effective as respiratory substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1976.tb01574.x
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  • 2
    Electronic Resource
    Electronic Resource
    [Letters to Nature] (1975)
    [s.l.] : Nature Publishing Group
    Nature 258 (1975), S. 748-749 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In describing their technique, Matus and Mughal state that they have used a "non-immune serum" diluted 1:50 for controls, and found only weak cytoplasmic autofluor-escence of neurones with the immunofluorescence procedure. This is hardly a sufficient control since such procedures require careful ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/258748a0
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  • 3
    Electronic Resource
    Electronic Resource
    14-3-2 protein in rat brain synapses (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 1094-1095 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of the 14-3-2 protein in rat brain synapses was studied by immuno electron microscopy. The protein was localized to the postsynaptic web and to the postsynaptic membrane, but was also prominent both in the presynaptic membrane and in the presynaptic densities. No significant activity was observed in the synaptic vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01945991
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  • 4
    Electronic Resource
    Electronic Resource
    Cathepsin D: Ultra-immunohistochemical localization in dentinogenesis (1979)
    Springer
    Calcified tissue international 29 (1979), S. 251-256 
    Details
    ISSN: 1432-0827
    Keywords: Cathepsin ; Calcification ; Dentinogenesis ; Proteoglycans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Cathepsin D was purified from rat liver using a new affinity chromatographic method, based on the coupling to the specific inhibitor pepstatin. This preparation was used for the production of specific antibodies from rabbit. The purified IgG fraction was conjugated to horseradish peroxidase in a two-step coupling procedure and used for electron microscopic immunohistochemistry of the odontoblast-predentine region of the rat incisor. Precipitates, indicating the presence of cathepsin D, were seen in the odontoblast, odontoblast process, and in the extracellular unmineralized matrix, the predentine. The observations are discussed in relation to proteoglycan degradation at the mineralization front simultaneous with crystal formation, and in relation to the function of lysosomal enzymes in the turnover of connective tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408088
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation of highly purified glomerular complexes from rabbit cerebellum (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 221-223 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Es wird eine neue Methode zur Präparation der sogenannten «glomerulären Komplexe» aus Kaninchen-Kleinhirn beschrieben, deren komplexe innere Struktur weitgehend intakt bleibt. Mit Hilfe der quantitativen elektronenmikroskopischen Methode wurden über 90% intakter Glomeruli errechnet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01990716
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural studies on blood-brain barrier dysfunction after cerebral air embolism in the rat (1978)
    Springer
    Acta neuropathologica 44 (1978), S. 53-56 
    Details
    ISSN: 1432-0533
    Keywords: Blood-brain barrier ; Air embolism ; Horseradish peroxidase ; Cerebral cortex ; Electron microscopy ; Carotid artery ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Male albino rats were anaesthetized with diazepam, injected with horseradish peroxidase and Evans blue-labeled albumin and given an embolus of 0.01 ml air in the right common carotid artery after ligation of the external carotid branch. The pial arteries of the right cerebral hemisphere were stained blue, particularly the middle cerebral artery and its main arterial branchlets. Ultrastructurally, some endothelial cells in the right middle cerebral artery, small arteries and arterioles showed a diffuse distribution of horseradish peroxidase in their cytoplasm, although these vessels only occasionally showed peroxidase in their basement membranes. Other endothelial cells in these arterial branchlets showed few if any signs of a diffuse distribution of peroxidase but displayed several pinocytotic vesicles and occasionally trans-endothelial channels filled with peroxidase, sometimes with a slight leakage of peroxidase into adjacent basement membranes and neuropil. Scattered pinocytotic vesicles were observed in capillaries and venules, but there was usually no extravasation of peroxidase around these vessels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691639
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  • 7
    Electronic Resource
    Electronic Resource
    Extravasation, spread and cellular uptake of Evans blue-labelled albumin around a reproducible small stab-wound in the rat brain (1976)
    Springer
    Acta neuropathologica 34 (1976), S. 125-136 
    Details
    ISSN: 1432-0533
    Keywords: Stab wound ; Extravasation ; Injury ; Blood-Brain-Barrier ; EBA Leakage ; Cellular damage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A small stab wound was made in the frontal lobe of the rat brain in order to study both the acute damage and the restitution of the blood-brain barrier to macromolecules under well-defined experimental conditions. Intravenously administered Evans blue-labelled albumin (EBA) was used as a tracer and the brain sections were observed in a fluorescence microscope. EBA leaked into the neuropil only during the first 3 days after the trauma. The maximal leakage occurred during the first day after the injury. The tracer spread from the area surrounding the stab wound in a reproducible way, initially roughly centrifugally. Nerve and glial cells close to the wound displayed a diffuse fluorescence of their cell bodies 1 to 6 h after the injury, i.e. at times with maximal extravasation of the tracer. A more granular distribution of the tracer was seen in neurons and glial cells at both very short and long times after EBA injection. Thus, the blood-brain barrier lesion induced was reproducible and reversible as judged by the pattern of EBA leakage. Some cells immediately adjacent to the injury had a diffuse cytoplasmic distribution of the tracer complex, in contrast to cells more distant from the injury, having a more granular distribution of the tracer in their cytoplasm. However, the appearance of the fluorescence in neurons and glia was to a large extent dependent upon the time after the injury, at which the cells were exposed to the tracer complex and on the time that had elapsed from the time of injection of the tracer complex to the sacrifice of the animal. Thus, it seems likely that two factors contribute to the appearance of the neuronal EBA distribution: on one hand the location and possibly extent of cellular damage of the cell, and on the other hand, the time and amount of EBA to which the cells were exposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684663
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  • 8
    Electronic Resource
    Electronic Resource
    Scanning electron microscopic studies of the rat incisor odontoblastema (1975)
    Springer
    Cell & tissue research 159 (1975), S. 233-243 
    Details
    ISSN: 1432-0878
    Keywords: Odontoblasts ; Predentine ; Dentine ; Calcification ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A scanning electron microscopic technique was used to investigate the surface structure of dentinogenically active odontoblasts. Thin pieces of rat incisors were fixed, rapidly frozen, freezedried at -70° C and fractured to expose new surfaces prior to examination in the SEM. Differences in the appearance of odontoblastic cell surfaces were seen, with the most extensive ridge formations at the distal part of the sides of the odontoblasts. The predentine area displayed a spongy structure which contrasted to the compact appearance of dentine. Results are discussed in relation to previous studies at the light microscopic and transmission electron microscopic levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219159
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  • 9
    Electronic Resource
    Electronic Resource
    Effects of barbiturates on ultrastructure and polymerization of microtubules in vitro (1975)
    Springer
    Cell & tissue research 162 (1975), S. 35-47 
    Details
    ISSN: 1432-0878
    Keywords: Microtubules ; Barbiturates ; Axonal transport ; Polymerization ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Barbiturates were examined for in vitro effects on ultrastructure of the frog sciatic system and polymerization of microtubules (MT) in a brain supernatant. Exposure for 5–17 h to 2.0 mM barbiturates caused a considerable loss of MT in ganglionic cell bodies and sciatic axons. This was mostly followed by a proliferation of 10 nm filaments. Under similar conditions treatment with 1 mM NaCN or 0.1 mM 2,4-DNP did not change the number or ultrastructure of MT and filaments. Eight barbiturates, varying in binding ratios to serum albumin and partition coefficients, were tested for effects on polymerization of MT using viscometry. Inhibitory effects were found which correlated with their reported ability to bind to albumin and brain fractions. Dimethylsulphoxide and ethanol were used as solvents for some of the barbiturates. These solvents at 1% had stabilizing effects on MT. The present results are discussed in relation to previous findings of inhibition of rapid axonal transport in vitro in the frog sciatic system by barbiturates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223260
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural localisation of proteoglycans in the odontoblast-predentin region of rat incisor (1976)
    Springer
    Cell & tissue research 168 (1976), S. 277-287 
    Details
    ISSN: 1432-0878
    Keywords: Proteoglycans ; Odontoblasts, predentin, dentin ; Calcification ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of proteoglycans in the predentin of the rat incisor was investigated by ultrastructural histochemistry. Ruthenium red stained the cell coat of the odontoblasts as well as intracellular vesicles. There was also a staining of the extracellular matrix, but not of collagen fibers in the predentin. Treatment with the enzyme hyaluronidase prior to staining with ruthenium red abolished the staining of the vesicles and the extracellular matrix but not that of the cell coat. Bismuth nitrate and phosphotungstic acid gave similar staining of odontoblast vesicles and extracellular matrix. It is likely that the stained structures contain proteoglycans. The importance of these proteoglycans and their ultrastructural localization are discussed in relation to intracellular transport and the calcification process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215306
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