Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1965-1969  (2)
  • Antikörper  (1)
  • Ouabain
  • Permeability
Source
Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Zur Frage der Bedeutung des Kininsystems beim thermischen Ödem der Rattenpfote (1969)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 264 (1969), S. 476-493 
    Details
    ISSN: 1432-1912
    Keywords: Kinins ; Permeability ; Heat ; Inflammation ; Kinine ; Permeabilität ; Hitze ; Entzündung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The suboutis of rat paws heated (46,5° C) in situ has been perfused. Kinin activity could be demonstrated regularly in the fluid which was collected in ice. When the solutions were tested immediately after having passed the tissue, only some of the experiments yielded positive results. Native and125Jlabelled kininogen as well as kininogenase and kininase activities passed into the perfusates. The sensitivity to dextran and the kinin release on heating were, in contrast to recent reports, not correlated. 2. The release of the components of the kinin system approximately paralleled that of labelled human albumin. Their concentration rose until about 1 hour after the start of the heating. There was no priority of the components of the kinin system when compared with human albumin which can be regarded as permeability indicator. 3. Intravenously injected carboxypeptidase B, because of its lower molecular weight, entered the interstitial fluid more easily than did the plasma carboxypeptidase N. Its blood level decreased rapidly; but sufficient tissue concentrations could be maintained by intravenous infusions. Neither the volume nor the time dependence of the thermic edema changed during carboxypeptidase B-infusions. The same was true for infusions of trasylol, whereas phenylbutazone inhibited the edema significantly. Edema formed by short heating (30 sec, 55° C) was equally resistant to carboxypeptidase B. 4. In the skin and muscles of the heated rat paw, carbon particles mainly stained the capillary walls. This finding argues against a considerable involvement of “classical” mediators which should induce venular lesions. 5. Infusion of large amounts of bradykinin into the arterial supply did not imitate the thermic edema; neither has bradykinin been found in the perfusate of the subcutis. 6. In the light of these findings, a significant role of the kinin system in the thermic edema of the rat paw is to be doubted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002384
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Solid-phase-Radioimmunassay und Blutspiegel von Staphylokokken-α-Toxin (1969)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 262 (1969), S. 165-182 
    Details
    ISSN: 1432-1912
    Keywords: Radioimmunassay ; Blood Level ; Staphylococcal Toxin ; Solid Phase ; Antibodies ; Radioimmunassay ; Blutspiegel ; Staphylokokken-Toxin ; Festphase ; Antikörper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. Es wird ein „solid-phase-radioimmunassay“ unter Verwendung von kovalent an Cellulose gebundenen Antikörpern entwickelt. Vorteile und Reaktionsbedingungen werden am Beispiel des Staphylokokken-α-Toxins dargestellt. 2. Einige der untersuchten Kaninchenseren, Humanplasma und vor allem natives antitoxisches Serum enthalten präcipitierende Antikörper, welche von der zugesetzten Radioaktivität um so mehr binden, je höher die Konzentration an unmarkiertem Toxin ist („inverser“ Radioimmunassay). 3. Unmarkiertes und markiertes Toxin verschwinden ungewöhnlich schnell (Halbwertszeit 〈 5 min) aus dem zirkulierenden Blut des Kaninchens. Eliminiertes markiertes Toxin läßt sich durch Gabe von Antitoxin nicht in die Blutbahn zurückholen. Vorherige Applikation von Normal-Rinderserum und von antitoxischem Rinderserum verzögert die Elimination des α-Toxins. 4. Nach doppelseitiger Nierenligatur wird markiertes Toxin erheblich langsamer aus der Blutbahn eliminiert; Ligatur beider Ureteren ist in dieser Hinsicht weniger effektiv.
    Notes: Summary 1. A solid-phase radioimmunassay has been developed which utilizes antibodies covalently bound to cellulose. Its advantages and reaction conditions have been demonstrated with staphylococcal α-toxin. 2. Sera of some rabbits, human plasma and especially native antitoxic sera contain precipitating antibodies which bind added radioactive toxin in proportion to the concentration of native toxin (“inverse” radioimmunassay). 3. Native and labelled toxins disappear very quickly from the circulating blood of rabbits (half-life time below 5 min). After its disappearance from the blood, radioactive toxin cannot be redistributed into the circulation by injection of antitoxin. Previous injection of normal or antitoxic bovine serum delays the elimination of α-toxin. 4. Previous ligation of both kidneys delays the elimination of labelled toxin from the blood stream considerably. Ligation of the ureters is less effective in this respect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00537657
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...