ZIB

Digitale Medien

Isolation of angiotensin-converting enzyme inhibitor from tuna muscle

Kohama, Y. ; Matsumoto, S. ; Oka, H. ; [weitere]

Amsterdam : Elsevier

Biochemical and Biophysical Research Communications 155 (1988), S. 332-337

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ISSN: 0006-291X

Schlagwort(e): [abr] ACE; angiotensin-converting enzyme ; [abr] AI; ACE inhibitor ; [abr] M. W.; molecular weight

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Physik

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/S0006-291X(88)81089-1

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Digitale Medien

Isolation of angiotensin-converting enzyme inhibitor from tuna muscle

Kohama, Y. ; Matsumoto, S. ; Oka, H. ; [weitere]

Amsterdam : Elsevier

Biochemical and Biophysical Research Communications 155 (1988), S. 332-337

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Details

ISSN: 0006-291X

Schlagwort(e): [abr] ACE; angiotensin-converting enzyme ; [abr] AI; ACE inhibitor ; [abr] M. W.; molecular weight

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Physik

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/S0006-291X(88)81089-1

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Digitale Medien

Comparative platelet anti-aggregant activity of D-cysteinolic acid analogues (1989)

Satake, M. ; Chiba, Y. ; Kohama, Y. ; [weitere]

Springer

Cellular and molecular life sciences 45 (1989), S. 1110-1112

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ISSN: 1420-9071

Schlagwort(e): D-Cysteinolic acid ; platelet aggregation ; inhibitor ; marine product ; 2-aminoethyl disulfide ; sardine

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary D-Cysteinolic acid (1) analogues with an S-C-C-N skeleton showed increased platelet anti-aggregant activity in the following order: 2-aminoethanesulfonic acids, thiazolidines, 2-aminoethanethiols and 2-aminoethyl disulfides. Methyl substitutions at the 2-position potentiated the activity. Of these analogues, bis [(R)-2-aminopropyl] disulfide was the most potent inhibitor of platelet aggregation, with about 600-fold the activity of (1).

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01950172

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Digitale Medien

Effect of glucose and phloretin-2′-β-D-glucose (phloridzin) on in vitro fertilization of mouse ova (1986)

Okabe, M. ; Adachi, T. ; Kohama, Y. ; [weitere]

Springer

Cellular and molecular life sciences 42 (1986), S. 398-399

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ISSN: 1420-9071

Schlagwort(e): In vitro fertilization ; mouse ova ; capacitation ; glucose ; phloretin-2′-β-D-glucose (phloridzin)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The fertilization ratio of mouse ova in vitro decreased when glucose concentration in the medium was lowered. However, the addition of phloretin-2′-β-D-glucose (phloridzin), known as a glucose uptake inhibitor, restored the fertilization ratio back to the control level. The glucose moiety of the phloridzin seemed to be responsible for this effect.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02118625

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Digitale Medien

Green Fluorescent Protein (GFP) as a Marker for Cell Viability After UV Irradiation (1999)

Baumstark-Khan, C. ; Palm, M. ; Wehner, J. ; [weitere]

Springer

Journal of fluorescence 9 (1999), S. 37-43

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ISSN: 1573-4994

Schlagwort(e): Green fluorescent protein (GFP) ; constitutive GFP expression ; visualization of GFP fluorescence ; UV exposure of mammalian cells ; Chinese Hamster Ovary (CHO) cell lines of different repair capabilities

Quelle: Springer Online Journal Archives 1860-2000

Thema: Physik

Notizen: Abstract Generation of Chinese Hamster Ovary (CHO) cell lines stably expressing green fluorescent protein (GFP) was achieved using a plasmid vector that encoded the red-shifted pCX-xGFP under the control of a strong hybrid promoter composed of a CMV enhancer and a β-actin/β-globin gene promoter. Cotransfection of the promoter-less pSV2-Neo helper plasmid transmitting neomycin resistance was followed by selection with the antibiotic G418. Constitutive GFP expression could be visualized in living and fixed cells using fluorescence spectroscopy, fluorescence microscopy, and flow cytometry. DNA repair-proficient (AA8) and deficient (UV5) CHO strains were used for survival tests after UVC irradiation. Cells carrying the GFP construct (AA8-pGFP, UV5-pGFP) show the same response to UV irradiation (colony forming ability) as their nontransformed parental cell lines (AA8, UV5). Using GFP as a marker for cell viability, cells were harvested after certain postirradiation growth periods and the numbers of GFP expressing cells and fluorescence intensities were determined by FACS analysis. Generally, GFP fluorescence in irradiated cells is not seen when cell membranes are damaged (leak-out of the soluble GFP). Irradiated cells without membrane damage express GFP continuously (leading to a dose-dependent increase in GFP contents).

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1020583623407

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Digitale Medien

Reconsiderations on singularity or crack tip elements (1979)

Yamada, Y. ; Ezawa, Y. ; Nishiguchi, I. ; [weitere]

Chichester [u.a.] : Wiley-Blackwell

International Journal for Numerical Methods in Engineering 14 (1979), S. 1525-1544

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ISSN: 0029-5981

Schlagwort(e): Engineering ; Engineering General

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Mathematik , Technik allgemein

Notizen: Various singularity finite elements proposed so far are reviewed and their correlations are discussed. The method adopted in the comparative study is based on the singularity mapping or transformation technique. It is suggested that a compact design of computational routines is materialized by using the element which embodies singularity transformation and can be compatible with the notion of using the variable-number-nodes element in the general-purpose computer programs.

Zusätzliches Material: 11 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/nme.1620141008

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