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  • Mitochondrion  (2)
  • Palmitoylcarnitine  (2)
  • Pisum  (2)
  • 3D reconstruction  (1)
  • 1
    ISSN: 1433-044X
    Keywords: Key words Supraspinatus outlet syndrome ; computed tomography ; 3D reconstruction ; shoulder ; acromion ; Schlüsselwörter Supraspinatus-outlet-Syndrom • CT • 3D-Rekonstruktion • Schulter • Akromion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eine mechanische Irritation der Supraspinatussehne aufgrund einer Einengung des subakromialen Raumes wird als mögliche Ursache des Supraspinatus-outlet-Syndroms angesehen. Ziel der vorliegenden Studie war die Entwicklung einer Methode zur verbesserten räumlichen Messung akromiohumeraler Distanzen und zur Beurteilung der Akromionform in der dreidimensional rekonstruierten Computertomographie (CT). An 7 Patienten mit einseitigem Supraspinatus-outlet-Syndrom wurde das Akromion und der subakromiale Raum aus hochauflösenden CT-Schnitten dreidimensional (3D-) rekonstruiert. Die akromiohumerale Distanz wurde in 3 rekonstruierten Frontalschnitten gemessen und auf der gesunden und erkrankten Seite verglichen. Die Akromionform wurde der Klassifikation nach Bigliani im konventionellen Röntgenbild gegenübergestellt. Die akromiohumerale Distanz war an der betroffenen Schulter nicht signifikant kleiner als auf der gesunden Seite. In 5 Fällen konnte die Klassifikation der Akromionform im Röntgenbild in der 3D-CT bestätigt werden, in 2 Fällen ergab sich eine abweichende Beurteilung. Die Ergebnisse legen nahe, daß mit der 3D-CT eine verbesserte Beurteilung der Akromionform und der akromiohumeralen Distanz möglich ist.
    Notes: Summary Mechanical irritations of the supraspinatus tendon due to a reduction of the subacromial space are considered to be a potential cause of supraspinatus outlet syndrome. The aim of the current study was, therefore, to provide a method of improved measurement of the acromiohumeral distance and the evaluation of the acromial shape. In seven patients aged 50–60 years with unilateral supraspinatus outlet syndrome, imaging with standardized, high-resolution computed tomography (CT) was performed in order to demonstrate the acromion and the subacromial space three-dimensionally. The acromiohumeral distance was measured in three reconstructed frontal sections, and the two sides of each patient were directly compared. The acromial shape was compared with the classification of Bigliani as derived from conventional radiography. Whereas the Constant Score was found to be significantly reduced in the diseased shoulders (p 〈 0.01), the acromiohumeral distance was not significantly lower in three standardized frontal sections. In five cases the classification of the acromial form from the conventional radiograph was confirmed by 3D CT, whereas in two cases it was shown to be different. Our results suggest that primary or secondary changes of the acromial form can be more accurately evaluated with 3D CT than with conventional radiography. A direct comparison of the two sides in patients with supraspinatus syndrome does not suggest a reduction of acromiohumeral distance in the diseased shoulder.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 196 (1995), S. 720-726 
    ISSN: 1432-2048
    Keywords: β-Oxidation ; Mitochondrion ; Palmitoylcarnitine ; Pisum ; Solanum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria from pea (Pisum sativum L.) cotyledons and potato (Solanum tuberosum L.) tubers exhibited a palmitoyl carnitine-dependent, KCN-sensitive stimulation of the oxygen uptake measured in the presence of 0.2mmol·−1 malate (sparker malate), provided a certain concentration range of palmitoylcarnitine was observed. Above this concentration range, which was dependent on the bovine serum albumin (BSA) concentration of the reaction mixture, the mitochondrial oxygen uptake was inhibited by palmitoylcarnitine. Palmitoylcarnitine (racemate) and palmitoyl-l-carnitine were equally effective in stimulating/inhibiting mitochondrial oxygen uptake in the presence of sparker malate. The mitochondrial membrane potential generated in the presence of sparker malate was partially dissipated by palmitoyl-lcarnitine concentrations stimulating the mitochondrial oxygen uptake. The formation of acid-soluble radioactivity in reaction mixtures provided with [1-14C]palmitoyll-carnitine was considerably lower than that expected minimally if the palmitoyl-l-carnitine-stimulated oxygen uptake resulted from palmitoyl-l-carnitine oxidation sparked by malate. Palmitoylcarnitine concentrations resulting in stimulation of the mitochondrial oxygen uptake in the presence of sparker malate also led to a stimulation of succinate-cytochrome c reductase activity, as well as to an increase in the measurable activities of mitochondrial matrix enzymes, indicating loss of both mitochondrial integrity and mitochondrial enzyme latency in the presence of palmitoylcarnitine. Correspondingly, malate-dependent NADH formation was stimulated by palmitoylcarnitine. Neither NAD reduction nor oxygen uptake were observed when the mitochondria were provided with palmitoylcarnitine only. The oxygen uptake due to glycine oxidation by mitochondria from green sunflower (Helianthus annuus L.) cotyledons was affected by palmitoylcarnitine in a similar manner to the oxygen uptake of pea cotyledon and potato tuber mitochondria in the presence of sparker malate. The results lead to the conclusion that the palmitoylcarnitine-dependent stimulation of mitochondrial oxygen uptake observed in the presence of sparker malate results substantially from an enhanced malate oxidation due to the detergent effect of palmitoylcarnitine on the mitochondrial membranes, rather than from palmitoylcarnitine β-oxidation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Planta 196 (1995), S. 720-726 
    ISSN: 1432-2048
    Keywords: β-Oxidation ; Mitochondrion ; Palmitoylcarnitine ; Pisum ; Solanum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria from pea (Pisum sativum L.) cotyledons and potato (Solanum tuberosum L.) tubers exhibited a palmitoyl carnitine-dependent, KCN-sensitive stimulation of the oxygen uptake measured in the presence of 0.2mmol·−1 malate (sparker malate), provided a certain concentration range of palmitoylcarnitine was observed. Above this concentration range, which was dependent on the bovine serum albumin (BSA) concentration of the reaction mixture, the mitochondrial oxygen uptake was inhibited by palmitoylcarnitine. Palmitoylcarnitine (racemate) and palmitoyl-l-carnitine were equally effective in stimulating/inhibiting mitochondrial oxygen uptake in the presence of sparker malate. The mitochondrial membrane potential generated in the presence of sparker malate was partially dissipated by palmitoyl-lcarnitine concentrations stimulating the mitochondrial oxygen uptake. The formation of acid-soluble radioactivity in reaction mixtures provided with [1-14C]palmitoyll-carnitine was considerably lower than that expected minimally if the palmitoyl-l-carnitine-stimulated oxygen uptake resulted from palmitoyl-l-carnitine oxidation sparked by malate. Palmitoylcarnitine concentrations resulting in stimulation of the mitochondrial oxygen uptake in the presence of sparker malate also led to a stimulation of succinate-cytochromec reductase activity, as well as to an increase in the measurable activities of mitochondrial matrix enzymes, indicating loss of both mitochondrial integrity and mitochondrial enzyme latency in the presence of palmitoylcarnitine. Correspondingly, malate-dependent NADH formation was stimulated by palmitoylcarnitine. Neither NAD reduction nor oxygen uptake were observed when the mitochondria were provided with palmitoylcarnitine only. The oxygen uptake due to glycine oxidation by mitochondria from green sunflower (Helianthus annuus L.) cotyledons was affected by palmitoylcarnitine in a similar manner to the oxygen uptake of pea cotyledon and potato tuber mitochondria in the presence of sparker malate. The results lead to the conclusion that the palmitoylcarnitine-dependent stimulation of mitochondrial oxygen uptake observed in the presence of sparker malate results substantially from an enhanced malate oxidation due to the detergent effect of palmitoylcarnitine on the mitochondrial membranes, rather than from palmitoylcarnitine β-oxidation.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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