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  • Cytoskeleton  (2)
  • 61.16. Ch  (1)
  • Carboxin  (1)
  • Cortical microtubules  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 62 (1996), S. 289-292 
    ISSN: 1432-0630
    Keywords: 61.16. Ch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Variable thickness bridges of superconducting Nb thin films have been fabricated for the first time by a combination of conventional photolithography and scanning force nanofabrication methods. The weak link behavior of these superconducting nanobridges was verified by low-temperature transport measurements, in particular by measuring the critical current as a function of an externally applied magnetic field. Future applications of miniaturized weak link structures may include highresolution scanning SQUID microscopes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Key words Basidiomycete ; Transformation ; White-rot fungi ; Carboxin ; Succinate:ubiquinone oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx R, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6102
    Keywords: Arabidopsis thaliana ; Cortical microtubules ; Cytoskeleton ; Green-fluorescent-α-tubulin 6 fusion protein ; Microtubule ; Transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary AGFP-TUA6 (α-tubulin 6) gene was transduced in theArabidopsis thaliana genome, and the GFP-TUA6 protein was expressed by 20% of the total α-tubulin amount. The expressed GFP-TUA6 protein was incorporated into cortical microtubules (cMTs), so that the cMTs could be visualized under the fluorescence microscope in the living cells. The rearrangement of cMTs was observed at the tangential epidermal cell face of the hypocotyl. At the initial stage of light-induced cMT rearrangement from a transverse to an oblique or a longitudinal orientation, randomly oriented short MTs appeared. These MTs rapidly elongated obliquely or longitudinally as the transverse cMTs shortened. Finally, the transverse cMTs were replaced by the newly organized oblique or longitudinal cMTs. Reorganization of the cMTs took 50–70 min. Treatment of seedlings with 5 × 10−5 M cytochalasin B induced disarrayed cMTs. The involvement of cytochalasin B in the orientation of developing MTs is discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 206 (1999), S. 201-206 
    ISSN: 1615-6102
    Keywords: Cytoskeleton ; Epidermal cell ; GFP-TUA6 fusion protein ; Microtubule ; Transgenic plants ; Tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Microtubules (MTs) were visualized in living cells of several tissues in transgenicArabidopsis thaliana. The transformed Arabidopsis plant was obtained by infecting it withAgrobacterium tumefaciens carrying the GFP-TUA6 plasmid. The fluorescence of the MTs was due to the fluorescence of GFP-TUA6 that was polymerized into the MTs. The distribution patterns of the visualized MTs in the living epidermal cells of leaves was similar to that in fixed epidermal cells. The actual destruction of MTs by oryzalin was observed in a living cell. Cytochalasin B exerts no effect on the distribution pattern of MTs. The fluorescence intensity of MTs was different among cells in different tissues.
    Type of Medium: Electronic Resource
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