ZIB

1

Digitale Medien

Bilayer thickness and enzymatic activity in the mitochondrial cytochrome c oxidase and ATPase complex

Montecucco, C. ; Smith, G.A. ; Dabbeni-sala, F. ; [weitere]

Amsterdam : Elsevier

FEBS Letters 144 (1982), S. 145-148

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ISSN: 0014-5793

Schlagwort(e): ATP-synthase ; Bilayer thickness ; Cytochrome oxidase ; Protein-lipid interaction

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Physik

Materialart: Digitale Medien

URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(82)80588-7

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2

Digitale Medien

Stability of a foreign gene in transgenic Nicotiana tabacum L. plants during a cycle of dedifferentiation/differentiation

Bellini, C. ; Giordani, C. ; Lupotto, E. ; [weitere]

Amsterdam : Elsevier

Plant Science 82 (1992), S. 193-200

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ISSN: 0168-9452

Schlagwort(e): Nicotiana tabacum ; dedifferentiation/differentiation ; gene stability ; kanamycin ; transformation

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0168-9452(92)90220-G

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3

Digitale Medien

Effect of fusicoccin on plant cell cultures and protoplasts (1977)

Rollo, F. ; Nielsen, E. ; Sala, F. ; [weitere]

Springer

Planta 135 (1977), S. 199-201

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ISSN: 1432-2048

Schlagwort(e): Cell suspension culture ; Fusicoccin ; 3-O-Methylglucose uptake ; Potassium ion uptake ; Proton extrusion ; Protoplasts

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract We have assayed the capacity of the fungal toxin fusicoccin to induce some of its characteristic effects (acidification of the medium, stimulation of K+, and of 3-O-methyl-D-glucose uptake) in cell suspensions of Parthenocissus tricuspidata (Siebold et Zucc.) Planchon, Acer pseudoplatanus L. and Oryza sativa L., and in protoplast suspensions prepared from leaves of Nicotiana tabacum L. and Spinacia oleracea L. or from cultures of P. tricuspidata. Evidence is presented showing that all tested biological materials respond to the addition of fusicoccin. The observation that the toxin is also active on protoplasts indicates that the cell wall is not involved in the mechanism of action of fusicoccin.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00387171

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4

Digitale Medien

Somaclonal variation versus chemically induced mutagenesis in tomato (Lycopersicon esculentum L.) (1987)

Gavazzi, G. ; Tonelli, C. ; Todesco, G. ; [weitere]

Springer

Theoretical and applied genetics 74 (1987), S. 733-738

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ISSN: 1432-2242

Schlagwort(e): Tomato ; Somaclonal variation ; Chemical mutagenesis ; Mutants

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary A comparison was made of the type and frequency of mutational events found in the progeny of tomato plants regenerated after one passage in vitro with those induced by chemical mutagenesis with ethyl methane sulphonate. Several mutants were recovered in the progeny of regenerated and mutagenized plants of two cultivars of tomato. They can be grouped into the following categories: seedling lethality, male sterility, resistance to Verticillium, short stature, change in number of lateral shoots or in leaf shape. The results indicate that the two sources of variability differ in their effect, changing the spectrum and frequency of the mutants as well as, at least in some cases, their pattern of segregation.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00247550

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5

Digitale Medien

Effect of repeated DNA sequences on direct gene transfer in protoplasts of Nicotiana plumbaginifolia (1989)

Marchesi, M. L. ; Castiglione, S. ; Sala, F.

Springer

Theoretical and applied genetics 78 (1989), S. 113-118

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ISSN: 1432-2242

Schlagwort(e): Nicotiana plumbaginifolia ; Protoplasts ; Gene transfer ; Repeated DNA sequences

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary Highly repeated nuclear DNA sequences from leaves of Nicotiana plumbaginifolia were cloned in pBR322 and tested for their effect on direct gene transfer in protoplasts of the same organism. Protoplasts were prepared from suspension cultures and were incubated in the presence of the plasmid pHP23 carrying the kanamycin resistance gene APH(3′)II and in the presence of the plasmids carrying the cloned sequence. DNA uptake was induced by a polyethyleneglycol (PEG) treatment. Out of the 22 tested clones, 3 significantly stimulated the frequency of appearance of transformed colonies. DNA was extracted from some of the kanamycin-resistant calli obtained by co-transformations. Dot-blots have shown that the stimulatory effect on transformation frequency is often accompanied by a consistent increase in integrated genes sequences.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00299763

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6

Digitale Medien

The histogenesis of somaclones from tomato (Lycopersicon esculentum Mill.) cotyledons (1988)

Monacelli, B. ; Altamura, M. M. ; Pasqua, G. ; [weitere]

Springer

Protoplasma 142 (1988), S. 156-163

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ISSN: 1615-6102

Schlagwort(e): Tomato ; Lycopersicon esculentum ; Somaclonal variation ; Plant regeneration

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary A histological study ofin vitro cultured cotyledonary expiants of tomato (Lycopersicon esculentum) was performed in order to determine the site (differentiated tissue or developing callus) and the mode of plant regeneration. Results have shown that callus develops at the excision sites of cotyledonary expiants and that shoots are formed exclusively within the unorganized callus: excision areas are the only morphogenetic sites and the proximal excision is the preferred site for plant regeneration. Shoots differentiate by organogenesis within the superficial region of the callus. Few neocambial cells cooperate in the neoformation. Origin from a single cell is highly unlikely since rarely observed single activated cells never developed into shoots. Regenerated plants may be chimeras if invitro culture induces genetic diversity in the initial cells.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01290872

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7

Digitale Medien

Hybrid genes in the analysis of transformation conditions (1987)

Negrutiu, I. ; Shillito, R. ; Potrykus, I. ; [weitere]

Springer

Plant molecular biology 8 (1987), S. 363-373

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ISSN: 1573-5028

Schlagwort(e): transformation ; MgCl2-PEG-electroporation ; competence ; protoplasts

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Direct gene transfer into plant protoplasts has been recently developed, and conditions for high frequency transformation of SR1 tobacco protoplasts established. In this paper we analyse numerous transformation parameters in a comparative study on SR1 Nicotiana tabacum and N. plumbaginifolia, and report on a simple chemical technique for very efficient protoplast transformation. It is based on the synergistic interaction of MgCl2 and PEG. The technique yielded up to 1400 transformants per 3×105 treated N. tabacum protoplasts (up to 4.8% of the survivors, late selected clones). Using N. plumbaginifolia, the frequencies were 10-fold lower, indicating that the ‘competence’ for transformation has a species-specific component.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00015814

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