Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Monoclonal antibody  (3)
  • Monoclonal antibodies  (2)
  • Alexander's diseases (AD)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Non-specific binding of mouse myeloma IgM immunoglobulins by human myelin sheaths and astrocytes (1986)
    Springer
    Acta neuropathologica 70 (1986), S. 284-288 
    Details
    ISSN: 1432-0533
    Keywords: Myelin sheaths ; Astroglial cells ; Mouse IgM ; GFAP ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The immunoreactivity of purified mouse myeloma IgM immunoglobulins (mouse IgM) to human myelin sheaths and astroglial cells was evaluated with the peroxidase-antiperoxidase method on paraffin-embedded tissues from human gliomas and areas of multiple sclerosis, and from normal human cerebrum, spinal cord and spinal nerve roots. The mouse IgM reacted positively with central and peripheral myelin sheaths and, as shown independently by others, with the cytoplasm of neoplastic and reactive astroglia. Parallel immunostaining of successive sections with an anti-glial fibrillary acidic protein (GFAP) serum and/or the anti-Leu 7 monoclonal antibody was of considerable assistance in identifying the immunoreactive elements and in distinguishing specific from non-specific immunostaining of myelin sheaths and astroglia. Pretreatment with normal human serum inhibited the non-specific binding by mouse IgM without altering GFAP and Leu 7 reactivities. The non-specific binding of mouse IgM to human myelin sheaths and astroglia can therefore be overcome, and the specificity of mouse IgM monoclonal antibodies retained, by the parallel immunostaining of successive sections with mouse IgM. If nonspecific binding by mouse IgM is found to occur, it can then be inhibited by preincubation with normal human serum without loss of specific antigenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686085
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Retinal S-antigen immunoreactivity in medulloblastomas (1988)
    Springer
    Acta neuropathologica 76 (1988), S. 204-207 
    Details
    ISSN: 1432-0533
    Keywords: S-Antigen ; Monoclonal antibodies ; Cerebellar medulloblastoma ; Photoreceptor cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of 16 cerebellar medulloblastomas were studied immunohistochemically using a four-step immunoperoxidase (PAP) method and a monoclonal antibody (MAbA9-C6) which defines an epitope of the retinal S-antigen, a protein known to occur in retinal photoreceptor cells and pinealocytes of the pineal gland as well as in retinoblastomas, pineocytomas and pineoblastomas. Immunopositivity was demonstrated in a variable number of tumor cells in 50% of the cases. This finding may be an indication of a differentiation potential of medulloblastomas along the photoreceptor cell lineage. Alternatively, it may simply indicate the non-specificity of the retinal antigen in the neoplastic state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688104
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Encephalopathy with rosenthal fibre formation in a sheep (1980)
    Springer
    Acta neuropathologica 50 (1980), S. 57-60 
    Details
    ISSN: 1432-0533
    Keywords: Alexander's diseases (AD) ; Encephalopathy ; Rosenthal fibers (RFs) ; Sheep
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the brain of a 4-year-old ewe extensive Rosenthal fiber (RF) formation was found in a subpial and subependymal location and in particular in the cerebellar white matter. Here, the RFs were concentrated around blood vessels, and there was diffuse myelin rarefaction. The animal had given birth to two lambs, which were still healthy at the age of 3 weeks when their mother died after a short illness with astasia and alternating states of somnolence and hyperexcitability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688535
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Immunohistochemical recognition of human neuroepithelial tumors by anti-Leu 7 (HNK-1) monoclonal antibody (1986)
    Springer
    Acta neuropathologica 69 (1986), S. 227-233 
    Details
    ISSN: 1432-0533
    Keywords: Leu 7 ; HNK-1 ; Monoclonal antibody ; Gliomas ; Neuroepithelial tumors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The immunoreactivity of the anti-Leu 7 (HNK-1) monoclonal antibody, a marker for natural killer cells, was evaluated with the peroxidase-antiperoxidase (PAP) method on sections of human paraffin-embedded tissues from 135 tumors of the central nervous system and five esthesioneuroblastomas. As shown independently by others, the antibody was found to react with most types of neoplastic neuroepithelial cells. Our findings indicate that the reaction is most often localized on the cytoplasmic membranes. The immunoreactive cell membranes were generally those of well-differentiated tumor cells and of neoplastic cells found in tumors that usually were not embryonal in nature. Parallel immunostaining either of the same or of successive sections with an anti-glial fibrillary acidic protein serum was of considerable assistance in discriminating between different immunoreactive cells, e.g., between astrocytes and cells presumed to be oligodendrocytes. Despite its cross-recognition of cells of various histogenesis, the anti-Leu 7 monoclonal antibody can, in well-defined circumstances, elucidate specific differential diagnostic problems involving neurogenic neoplasms that cannot be resolved with routine staining techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688298
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Immunohistochemical recognition of human nerve sheath tumors by anti-Leu 7 (HNK-1) monoclonal antibody (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 319-324 
    Details
    ISSN: 1432-0533
    Keywords: Anti-Leu 7 ; HNK-1 ; Monoclonal antibody ; Myelin-associated glycoprotein ; Schwannoma ; Neurofibroma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using relatively high dilutions of anti-Leu 7 monoclonal antibody and a four-step peroxidase-antiperoxidase (PAP) reaction in paraffin-embedded tissues, we tested the affinity of this antibody to the cells of 47 human nerve sheath tumors and 22 other tumors in which the differential diagnosis with nerve sheath neoplasms is known to arise. Of all the nerve sheath tumors studied 68%, including 80% of the schwannomas, contained anti-Leu 7-positive cells. All 22 non-schwannian neoplasms were entirely, negative. Specimens of eight experimental malignant rat schwannomas were also negative for anti-Leu 7 antibody. Our findings suggest that anti-Leu 7 monoclonal antibody is a promising marker that may facilitate the differential diagnosis between human Schwann cell and non-schwann cell neoplasms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690835
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    S-Antigen immunoreactivity in human pineal glands and pineal parenchymal tumors. A monoclonal antibody study (1986)
    Springer
    Acta neuropathologica 71 (1986), S. 224-227 
    Details
    ISSN: 1432-0533
    Keywords: S-Antigen ; Monoclonal antibody ; Pineal gland ; Pineocytoma ; Pineoblastoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using a four-step immunoperoxidase (PAP) method and the monoclonal antibody MAbA9-C6 (MAbA9-C6), which defines an epitope of the retinal S-antigen (S-Ag), we investigated the S-Ag immunoreactivity in human fetal, newborn, infantile and adult pineal glands and in 13 human pineal parenchymal tumors. S-Ag immunoreactivity was demonstrated in a few cells in one of the four fetal and in both infantile glands. Eight of nine adult pineal glands contained isolated MAbA9-C6-positive cells. In two of seven pineocytomas showing neuronal or gangliogliomatous differentiation a few scattered cells displayed S-Ag positivity; two of four pineoblastomas contained small groups of strongly immunoreactive neoplastic cells; two malignant pineocytomas did not demonstrate any S-Ag immunoreactivity. Our results indicate that isolated cells in human pineal gland retain some of the cytochemical characteristics of photoreceptor cells recognized by the MAbA9-C6, and that S-Ag immunoreactivity may be occasionally expressed in pineal parenchymal tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688043
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...