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  • Antiproliferative drugs  (1)
  • Dermal papilla cell  (1)
  • Stewart-Bluefarb-Syndrom  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Expression of vascular endothelial growth factor (VEGF) in various compartments of the human hair follicle (1998)
    Springer
    Archives of dermatological research 290 (1998), S. 661-668 
    Details
    ISSN: 1432-069X
    Keywords: Key words Angiogenesis ; Human hair follicle ; Dermal papilla cell ; VEGF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Hair follicle vascularization appears to be closely related to the processes involved in hair cycle regulation, in which growth factors, cytokines and other bioactive molecules are involved. In particular, vascular endothelial growth factor (VEGF), essential for angiogenesis and vascular permeability, may be responsible for maintaining proper vasculature around the hair follicle during the anagen growth phase. The aim of our study was to compare the in vitro angiogenic capacity, i.e. the steady-state expression of the VEGF gene, of different cultured cell types derived from normal human hair follicles, corresponding to different follicular compartments. Human dermal papilla cells (DPC), fibrous sheath fibroblasts, dermal fibroblasts, and follicular and interfollicular keratinocytes were cultured and studied in vitro for VEGF expression at the mRNA level using RT-PCR, and for VEGF protein synthesis by radioimmunoprecipitation and immunocytochemistry. In vivo examination for VEGF expression in human terminal hair follicles was performed using immunohistochemical methods. In the present report the expression of four different VEGF molecular isoforms, differing in their angiogenic capacity, are described in different cultured follicular cell types for the first time. Cultured follicular cells strongly expressed mRNA of four VEGF molecular species identified as the 121-, 145-, 165- and 189-amino acid splice variants, the most prominent being the 121-amino acid molecule. DPC, and also other mesenchymal cells such as fibrous sheath fibroblasts and dermal fibroblasts, in vivo and in vitro strongly expressed VEGF mRNA and synthesized a 46-kDa VEGF protein, whereas follicular and interfollicular keratinocytes in vitro expressed lower levels of VEGF mRNA and proteins than mesenchymal cells. As the highest expression of VEGF was found in DPC, we suggest that DPC are mainly responsible for angiogenic processes possibly related to the human hair cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050370
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  • 2
    Electronic Resource
    Electronic Resource
    The phospholipid analogue hexadecylphosphocholine (HePC) inhibits proliferation of keloid fibroblasts in vitro and modulates their fibronectin and integrin synthesis (1997)
    Springer
    Archives of dermatological research 289 (1997), S. 164-169 
    Details
    ISSN: 1432-069X
    Keywords: Key words Hexadecylphosphocholine ; Keloid ; Hypertrophic scars ; Antiproliferative drugs ; Fibronectin ; Integrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Hexadecylphosphocholine (HePC), a topically effective compound, exerts a strong antiproliferative effect on neoplastic cells. In the present study we investigated (1) the antiproliferative effect of HePC on benign mesenchymal cells in vitro, using as examples normal and keloid fibroblasts, and (2) the influence of HePC on various functional properties of these cells, including phosphatidylcholine biosynthesis, their capacity to reorganize a three-dimensional collagen-I matrix, and their expression and synthesis of fibronectin and subunits of the β 1 integrin family. Fibroblasts derived from keloids (kelfib) and from normal skin (nfib) were cultured in serum-containing medium and treated in the third passage with 50 μmol/l HePC. Proliferative activity was significantly ( P 〈 0.05) more strongly inhibited in kelfib than in nfib under HePC treatment, whereas their phosphatidylcholine synthesis was inhibited to a similar extent. However, the ability of fibroblasts to contract a three-dimensional collagen-I lattice was significantly ( P 〈 0.05) enhanced only in kelfib treated with HePC. These functional differences following treatment with HePC were paralleled by an upregulation of the α 2 - and β 1 -integrin chains, and a downregulation of fibronectin synthesis and the α 5 -subunit. Our results indicate a differential modulation of kelfib metabolism by HePC, suggesting a possible new therapeutic approach for keloid and hypertrophic scars in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050173
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  • 3
    Electronic Resource
    Electronic Resource
    Kaposiforme, HHV-8-negative Akroangiodermatitis bei chronisch-venöser Insuffizienz (1999)
    Springer
    Der Hautarzt 50 (1999), S. 208-213 
    Details
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Akroangiodermatitis ; Morbus Mali ; Stewart-Bluefarb-Syndrom ; Pseudo-Kaposi-Sarkom ; Kaposi-Sarkom ; Key words Acroangiodermatitis ; Morbus Mali ; Stewart-Bluefarb-syndrome ; Pseudo-Kaposi’s-sarcoma ; Kaposi’s sarcoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A 76-year-old female patient developed severe manifestations of a kaposi-like acroangiodermatitis (so-called Mali’s disease) due to chronic venous insufficiency of the lower extremities. The patient presented with large areas of confluent, violaceous or brown-black papules on both lower legs. Histologically, proliferation of thick-walled capillaries was seen in the upper dermis consisting of fully differentiated endothelial cells, as shown by immunohistochemistry. In contrast to true Kaposi’s sarcoma, human-herpes-virus-8 DNA could not be detected by polymerase-chain-reaction in this condition. We review the diagnostic criteria used to distinguish between acroangiodermatitis, also called pseudo-Kaposi’s sarcoma, and the true Kaposi’s sarcoma.
    Notes: Zusammenfassung Wir berichten über eine 76jährige Patientin mit einer ausgeprägten kaposiformen Akroangiodermatitis (Morbus Mali), die sich klinisch mit großflächig konfluierenden, lividroten bis bläulich-schwarzen Papeln an beiden Unterschenkeln manifestierte und auf einer chronisch-venösen Insuffizienz Grad II nach Widmer mit Stammvarikosis Grad IV nach Hach sowie Parva- und Perforansinsuffizienzen bereits beruhte. Histologisch fanden sich, beschränkt auf die obere Dermis, prominente, dickwandige Kapillarproliferate, deren Endothelzellen immunhistologisch als ausgereift identifiziert werden konnten. In der Polymerase-Ketten-Reaktion war das Humane Herpes-Virus-8, das neuerdings beim klassischen und HIV-assoziierten, echten Kaposi-Sarkom nachgewiesen wurde, nicht nachweisbar. Das Krankheitsbild war bei unserer Patientin so ausgeprägt, daß wir hier unter Berücksichtigung der neueren Literatur erneut auf die Kriterien zur Abgrenzung der kaposiformen Akroangiodermatitis vom Kaposi-Sarkom eingehen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001050050891
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    Paper (German National Licenses)
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