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  • Aspergillus nidulans  (1)
  • Cloning  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans (1997)
    Springer
    Current genetics 32 (1997), S. 408-412 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Key words APS kinase ; ATP sulphurylase ; Aspergillus nidulans ; Allosteric inhibition
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002940050295
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD) (1986)
    Springer
    Molecular genetics and genomics 202 (1986), S. 271-275 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Aspergillus ; Isocitrate lyase ; Cloning ; Recombinant DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation of A. nidulans. Plasmids rescued in Escherichia coli were able to transform five different A. nidulans acuD mutants. Transformation using plasmids containing the cloned fragment resulted in integration at the acuD locus in six of nine transformants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00331649
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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