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  • Atomic, Molecular and Optical Physics  (2)
  • Embolisation  (2)
  • Japanese  (2)
  • Key wordsAgrobacterium  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Heterozygosities and allelic frequencies of 358 dinucleotide-repeat marker loci in the Japanese population (1998)
    Springer
    Journal of human genetics 43 (1998), S. 165-168 
    Details
    ISSN: 1435-232X
    Keywords: Key words Microsatellite ; Heterozygosity ; Japanese ; population
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We examined 64 normal Japanese chromosomes to determine the heterozygosities and allelic frequencies of 358 dinucleotide-repeat marker loci spanning the whole human genome. Comparisons of the data for each marker in the Japanese population sample with data for the same markers among Caucasian samples in the Genome Database (GDB) revealed a slightly lower average of heterozygosity in Japanese (71% vs 79%). Although the majority of the markers were as informative as in Caucasians, some in our sample were uninformative due to low heterozygosity; 38 loci revealed heterozygosities lower than 50% and 11 of these were less than 30%. Furthermore, allelic distributions at many of the marker loci were quite different in the two racial groups. Since such differences will influence statistical analyses between markers and disease loci, our data will be essential for linkage analyses, sib-ship pair analyses, and association studies involving the Japanese population. Therefore we have archived this database on a home page on the Internet (http://www.ims.u-tokyo.ac.jp/nakamura/Yamane.html).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050062
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Magnetic resonance signal intensity and volume changes after endovascular treatment of intracranial aneurysms causing mass effect (1998)
    Springer
    Neuroradiology 40 (1998), S. 184-188 
    Details
    ISSN: 1432-1920
    Keywords: Key words Aneurysm intracranial ; Magnetic resonance imaging ; Embolisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To determine when and how intracranial aneurysms causing mass effect change following endovascular treatment, we used MRI to assess patients for 2–3 years after the interventional procedure. Nine patients who had aneurysms compressing the surrounding structures underwent endovascular treatment. Proximal occlusion of the parent artery was performed in seven cases, and in two the aneurysm was embolised with microcoils. After embolisation, signal intensity within aneurysms tended to be high on both T1- and T2-weighted images. When there was rapid reduction in size high-signal zones within aneurysms became isointense or gave low signal on T1-weighted images. On T2-weighted images, isointense or low-signal foci appeared within high-signal areas in the aneurysm, giving mixed intensity. In typical cases, the mean volume of the aneurysm fell to approximately 30 % of its initial value 2–12 months after treatment. After this, no additional reduction was observed. The aneurysms which showed little signal intensity change tended to shrink more slowly and to a lesser degree than the more typical cases. Aneurysms which gave high signal on both T1- and T2-weighted images early following embolisation shrank more quickly than those showing little signal change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002340050565
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Dual microcatheter and guidewire snare technique for retrieval of intravascular foreign body: technical note (1996)
    Springer
    Neuroradiology 38 (1996), S. 615-617 
    Details
    ISSN: 1432-1920
    Keywords: Key words Caroticocavernous fistula ; Complication ; Embolisation ; Mechanical detachable coil ; Snare wire
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A transjugular snare technique was used to retrieve a stretched, residual mechanical detachable coil which extended from the cavernous sinus to the femoral vein, obliterating the transpetrosal route for further embolisation. The coil was snared by a microguidewire. Our new technique is described in this paper.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002340050319
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of soybean b-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants (1999)
    Springer
    Plant cell reports 18 (1999), S. 527-532 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium ; Kiwifruit ; β-1 ; 3-Endo-glucanase ; Transformation ; Disease tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Kiwifruit was transformed with a soybean β-1,3-endoglucanase (EC 3.2.1.39) cDNA under the control of the cauliflower mosaic virus (CaMV) 35S RNA promoter. The introduced gene was expressed in young leaves of the transformants. Assays of protein extracts from young leaves showed an increase in enzyme activity in many transformants, the transformant with the highest level of enzyme activity having an about sixfold increase over the control plants. When leaves from control and three transformants were inoculated with Botrytis cinerea, which causes gray mold disease, the disease lesion areas for two transformants were smaller than on control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050616
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated transformation and plant regeneration from hypocotyl segments of Japanese persimmon (Diospyros kaki Thunb) (1998)
    Springer
    Plant cell reports 17 (1998), S. 435-440 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium ; Hypocotyl ; Japanese persimmon ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypocotyl segments from the seeds of Japanese persimmon (Diospyros kaki Thunb) were cultured on a modified Murashige and Skoog medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea, zeatin or 6-benzylaminopurine. The highest frequency of shoot regeneration was observed when the segments were cultured on medium containing 2 mg/l of zeatin. This culture system was adapted to Agrobacterium-mediated transformation. The hypocotyl segments were inoculated with Agrobacterium tumefaciens strains harboring binary vectors, which contained the neomycin phosphotransferase II gene and the β-glucuronidase gene. Regenerated shoots were selected on a medium containing kanamycin. Histochemical GUS assay showed that the shoots regenerated from the segments inoculated with EHA101/pSMAK251 expressed the gus gene. The presence and integration of the gus gene was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. The regeneration frequency of transformed shoot was 11.1%. The transgenic shoots were rooted and developed into whole plants within 4–5 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050421
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Conformational studies on L-serine phosphate and hydrated L-serine phosphate using ab initio SCF calculations (1989)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 35 (1989), S. 395-407 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Ab initio MO-LCAO-SCF calculations using an STO-3G basis set were performed to find the most stable conformations of L-serine phosphate and hydrated L-serine phosphate. The most favorable conformation of L-serine phosphate is found to be one where the bond sequence O—C—C—C is trans and P—O—C—C gauche, and a very short hydrogen bond is formed between an oxygen atom of the phosphate group and a hydrogen atom of the ammonium group.For hydrated L-serine phosphate, a bridge-type hydration in which a water molecule links a phosphate oxygen and an ammonium hydrogen displays particularly low energy. In the four-hydrated L-serine phosphate anion, the most favorable conformation is such a bridged one having a rather extended configuration with regard to the bond sequences O—C—C—C and P—O—C—C.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560350305
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  • 7
    Electronic Resource
    Electronic Resource
    Ab initio SCF study of the interaction between L-serine phosphate and ammonia (1989)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 36 (1989), S. 587-598 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The possible conformational changes of L-serine phosphate due to the interaction with ammonia are investigated by means of ab initio MO-LCAO-SCF calculations, using a supermolecule approach and an STO-3G basis set. The most favorable conformation of a four-hydrated L-serine phosphate anion is found to be changed by the binding of an ammonium ion. Cointeraction of ammonia and NH4+ suggests another conformational change through the displacement of the bridging water molecule of the polyhydrated L-serine phosphate anion.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560360506
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Novel polymorphisms of prostate-specific antigen (PSA) gene associated with PSA mRNA expression in breast cancer (2000)
    Springer
    Journal of human genetics 45 (2000), S. 363-366 
    Details
    ISSN: 1435-232X
    Keywords: Key words Prostate-specific antigen gene ; Polymorphism ; Promoter ; Breast cancer ; Japanese ; Genotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A growing body of evidence suggests that prostate-specific antigen (PSA) is a novel prognostic factor for breast cancer. The molecular mechanism of variant PSA expression in breast cancer has remained poorly understood in spite of intensive research. Previous studies have shown that the coding region of the PSA gene is not a target for mutations in prostate cancer and breast cancer. The purpose of this study was to analyze genetic variations in the promoter region of the PSA gene, and to detect whether such variations are correlated with PSA mRNA expression in breast tumors. We identified two polymorphisms in the proximal promoter region of the PSA gene. These polymorphisms are located at positions −252 (G or A) and −205 (A or AA), and generate three genotypes. The genotypes were associated with PSA mRNA expression. Our findings suggest that these polymorphisms identified in the proximal promoter region may affect the transcriptional activity of PSA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380070009
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    Paper (German National Licenses)
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