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  • 1
    Electronic Resource
    Electronic Resource
    TMV resistance gene N homologues are linked to Synchytrium endobioticum resistance in potato (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 379-386 
    Details
    ISSN: 1432-2242
    Keywords: Key words Plants ; Disease resistance ; Host-pathogen interaction ; Synchytriumendobioticum ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The fungus Synchytrium endobioticum, the causal agent of potato wart disease, is subject to world-wide quarKantine regulations due to the production of persistent resting spores and lack of effective chemical control measures. The selection of Synchytrium-resistant potato cultivars may be facilitated by using markers closely linked with a resistance gene or by transferring a cloned gene for resistance into susceptible cultivars. Sen1, a gene for resistance to Synchytrium endobioticum race 1, was localized on potato chromosome XI in a genomic region which is related to the tobacco genome segment harbouring the N gene for resistance to TMV. Using N as probe, we isolated homologous cDNA clones from a Synchytrium-resistant potato line. The N-homologous sequences of potato identified by RFLP mapping a family of resistance gene-like sequences closely linked with the Sen1 locus. Sequence analysis of two full-length N-homologous cDNA clones revealed the presence of structural domains associated with resistance gene function. One clone (Nl-25) encodes a polypeptide of 61 kDa and harbours a Toll-interleukin like region (TIR) and a putative nucleotide binding site (NBS). The other clone (Nl-27) encodes a polypeptide of 95 kDa and harbours besides the TIR and NBS domains five imperfect leucine-rich repeats (LRRs). Both clones have at their amino terminus a conserved stretch of serine residues that was also found in the N gene, the RPP5 gene from Arabidopsis thaliana and several other resistance gene homologues, suggesting a function in the resistance response. Cloning of the disease resistance locus based on map position and the establishment of PCR-based marker assays to assist selection of wart resistant potato genotypes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051083
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1001-1007 
    Details
    ISSN: 1432-2242
    Keywords: Key words Chromosome identification ; Physical mapping ; FISH ; BAC ; Molecular cytogenetics ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Reliable and easy to use techniques for chromosome identification are critical for many aspects of cytogenetic research. Unfortunately, such techniques are not available in many plant species, especially those with a large number of small chromosomes. Here we demonstrate that fluorescence in situ hybridization (FISH) signals derived from bacterial artificial chromosomes (BACs) can be used as chromosome-specific cytogenetic DNA markers for chromosome identification in potato. We screened a potato BAC library using genetically mapped restriction fragment length polymorphism markers as probes. The identified BAC clones were then labeled as probes for FISH analysis. A set of 12 chromosome-specific BAC clones were isolated and the FISH signals derived from these BAC clones serve as convenient and reliable cytological markers for potato chromosome identification. We mapped the 5S rRNA genes, the 45S rRNA genes, and a potato late blight resistance gene to three specific potato chromosomes using the chromosome-specific BAC clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051573
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Construction of a potato YAC library and identification of clones linked to the disease resistance loci R1 and Gro1 (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 954-960 
    Details
    ISSN: 1432-2242
    Keywords: Key words Potato ; YAC library ; Disease resistance ; R1 ; Gro1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A yeast artificial chromosome (YAC) library was constructed from high-molecular-weight DNA of potato (Solanum tuberosum). Potato DNA fragments obtained after complete digestion with four different rare-cutter restriction enzymes were cloned using the pYAC-RC vector. The library consists of 21 408 YAC clones with an average insertion size of 140 kb. The frequency of YAC clones having insertions of chloroplast or mitochondrial DNA was estimated to be 0.5% and 0.3%, respectively. The YAC library was screened by PCR with 11 DNA markers detecting single genes or small gene families in the potato genome. YACs for 8 of the 11 markers were detected in the library. Using 2 markers that are linked to the resistance genes R1 and Gro1 of potato, we isolated two individual YAC clones. One of these YAC clones was found to harbour one member of a small family of candidate genes for the nematode resistance gene Gro1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050647
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    Paper (German National Licenses)
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