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  • Carcinoembryonic antigen  (2)
  • Freeze-fracture  (1)
  • Immunocytochemistry  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Secretion of calcitonin and carcinoembryonic antigen in long-term organ culture of human medullary thyroid carcinoma: Biochemical and immunocytochemical studies (1985)
    Springer
    Journal of molecular medicine 63 (1985), S. 205-210 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Calcitonin ; Carcinoembryonic antigen ; Medullary thyroid carcinoma in vitro ; Organ culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Tissue cultures of four C-cell carcinomas (medullary thyroid carcinoma, MTC) were prepared to study the basal and stimulated calcitonin (CT) and carcinoembryonic antigen (CEA) release. Immunohistological staining of the explants for CT and CEA have been performed after various periods of culture. These MTC explants were able continuously to release CT and CEA for periods up to 157 days. The spontaneous CT and CEA release decreased sharply during the 1st week of culture, then remained nearly constant over the observation period. The CEA/CT secretion ratio slightly declined during long-term culture; CEA release seems to drop earlier than CT production. CT and CEA could be detected in the same cells by immunocytochemical technique. The septal tissue consisting of dense connective tissue and amyloid produced by tumor cells seemed to increase during long-term culture. CT, but not CEA, was stimulated by pentagastrin (10−5 M), glucagon (6×10−6 M), and dose related by calcium (2.5–20 mM) in vitro. The MTC explant organ long-term culture proved to be a useful model for studies of human CT and CEA secretion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01731170
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  • 2
    Digitale Medien
    Digitale Medien
    Renin immunocytochemistry of the differentiating juxtaglomerular apparatus (1981)
    Springer
    Anatomy and embryology 162 (1981), S. 173-181 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Renin ; Juxtaglomerular apparatus ; Immunocytochemistry ; Fetal kidney ; Renin-angiotensin-system (RAS)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The differentiation of the juxtaglomerular apparatus in fetuses and newborn mice was investigated by renin immunocytochemistry and electron microscopy. Three to four days before delivery and prior to other organs renin was found in the fetal kidney. At this early time immunoreactivity was preferentially located in cells of the media of interlobular arteries. In newborn mice the formation of new nephrons and maturation of their glomeruli was accompanied by a shift in renin localization from the interlobular arteries to the afferent arterioles. At the same time, kidney renin content and concentration increased rapidly. Synchronously with renin immunoreactivity, during the capillary loop stage of glomerular development, granulated epitheloid cells became visible in the afferent arteriole.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00306489
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  • 3
    Digitale Medien
    Digitale Medien
    Metastatic C-cell carcinoma: Calcitonin and CEA production in monolayer culture (1986)
    Springer
    Journal of cancer research and clinical oncology 111 (1986), S. 284-288 
    Details
    ISSN: 1432-1335
    Schlagwort(e): C-cell carcinoma ; Monolayer culture ; Calcitonin ; Carcinoembryonic antigen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Mechanically dissociated cells from a surgically removed mediastinal C-cell carcinoma (MTC) were cultured over a period of 4 months. The cells of the monolayer culture consisted of clusters of small epithelial-like cells. Using semithin and ultrathin sections, two different types of cells could be characterized by shape of nucleus and by content and distribution of secretory granules. One type of cell showed a more irregularly shaped nucleus, the other contained a large oval nucleus, similar to the normal C-cell of the human thyroid. Calcitonin (CT) and carcinoembryonic antigen (CEA) were measured in supernatants in duplicate by radioimmunoassays. Radioimmuno-detectable CT levels in the supernatant of culture medium varied between 0.8 and 1.6 ng/ml and CEA levels between 5 and 27 ng/ml during a 2-month period. The present study proves that in monolayer-cultured cells of a MTC, metastases continue to produce radioimmuno-detectable CT and CEA. Whether the two different cell types in culture are relevant for carcinoma needs further investigation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00389246
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  • 4
    Digitale Medien
    Digitale Medien
    Gap junctions between pinealocytes (1981)
    Springer
    Cell & tissue research 218 (1981), S. 303-314 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pineal gland ; Freeze-fracture ; Cell junctions ; Gap junction ; Tight junction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The intercellular junctions between the pinealocytes of male rats were investigated by freeze-fracture and conventional electron microscopy. Our findings reveal that the intercellular contacts between pineal cells, formerly described as zonulae adhaerentes or zonulae occludentes, are in fact gap junctions which are difficult to characterize in thin sections due to their peculiar geometrical arrangement, which is in the form of “fenestrated” communicating zonules. The arrangement of these communicating zonules around rudimentary lumina of pineal clusters and rare transitions between tight and gap junctions may point to phylogenetic transformations of occluding into communicating zonules, corresponding with the change of the pineal gland from a sensory to a secretory organ. Alternatively, these tight-to-gap junctional transitions may reflect the periodic (circadian or seasonal) activity of the pineal gland.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00210346
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