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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 1018 (1990), S. 263-266 
    ISSN: 0005-2728
    Keywords: (Bacteria) ; Methanogenesis ; Protonmotive force ; Sodium motive force
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 892 (1987), S. 207-212 
    ISSN: 0005-2728
    Keywords: (M. barkeri) ; ATP synthesis-uncoupler ; ATPase,H^+- ; Membrane potential ; Methanogenesis ; Trimethylamine
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 215 (1987), S. 53-57 
    ISSN: 0014-5793
    Keywords: (M. barkeri) ; Methanogenesis ; Proton translocation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 148 (1987), S. 107-114 
    ISSN: 1432-072X
    Keywords: Autotrophy ; Clostridia ; Clostridium aceticum ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A plasmid screening was performed on 150 strains out of 75 clostridial species using a modification of the alkaline-lysis procedure. In 26 strains representing 21 species one or more plasmid bands were detected ranging in size from 3 to more than 100 kilobase pairs. Clostridium aceticum proved to contain a single small plasmid (pCA1) of 5.4 kbp as revealed by restriction analysis and electron microscopy. A physical map of pCA1 has been constructed. Spontaneous mutants of C. aceticum defective in autotrophic growth have been isolated. No direct correlation between plasmid content and autotrophy could be found.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 155 (1991), S. 272-277 
    ISSN: 1432-072X
    Keywords: Archaebacteria ; Methanogenesis ; Membranes ; Proton translocation ; ATP synthesis ; Electrontransport phosphorylation ; Hydrogenase ; F420 ; Heterodisulfide reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Washed everted vesicles of the methanogenic bacterium strain Gö1 catalyzed an H2-dependent reduction of the heterodisulfide of HS-CoM (2-mercaptoethanesulfonate) and HS-HTP (7-mercaptoheptanoylthreonine phosphate) (CoM-S-S-HTP). This process was independent of coenzyme F420 and was coupled to proton translocation across the cytoplasmic membrane into the lumen of the everted vesicles. The maximal H+/CoM-S-S-HTP ratio was 2. The tranmembrane electrochemical gradient thereby generated was shown to induce ATP synthesis from ADP+Pi, exhibiting a stoichiometry of 1 ATP synthesized per 2 CoM-S-S-HTP reduced (H+/ATP=4). ATP formation was inhibited by the uncoupler 3,5-di-tert-butyl-4-hydroxy-benzylidene-malononitrile (SF 6847) and by the ATP synthase inhibitor N,N′-dicyclohexylcarbodiimide (DCCD). This energy-conserving system showed a stringent coupling. The addition of HS-CoM and HS-HTP at 1 mM each decreased the heterodisulfide reductase activity to 50% of the control. Membranes from Methanolobus tindarius showed F420H2-dependent but no H2-dependent heterodisulfide oxidoreductase activity. Neither of these activities was detectable in membranes of Methanococcus thermolithotrophicus.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 133 (1982), S. 230-235 
    ISSN: 1432-072X
    Keywords: Methanosarcina barkeri ; Acetate ; Trimethylamine ; Methanogenesis ; Acetate oxidation ; Assimilation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During growth of Methanosarcina barkeri strain Fusaro on a mixture of trimethylamine and acetate, methane production and acetate consumption were biphasic. In the first phase trimethylamine (33 mmol x l-1) was depleted and some acetate (11–14 from 50 mmol x l-1) was metabolized simultaneously. In the second phase the remaining acetate was cleaved stoichiometrically into CH4 and CO2. Kinetic experiments with (2-14C)acetate revealed that only 2.5% of the methane produced in the first phase originated from acetate: 18% of the acetate metabolized was cleaved into CH4 and CO2, 23% of the acetate was oxidized, and 55% was assimilated. Methane produced from CD3−COOH in the first phase consisted of CD2H2 and CD3H in a ratio of 1:1.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 128 (1981), S. 288-293 
    ISSN: 1432-072X
    Keywords: Clostridium aceticum ; Acetic acid formation ; Chemolithotrophic growth ; Hydrogen uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spores of Clostridium aceticum (Wieringa) from 1947 have been revived, and a study for a redescription of this microorganism has been carried out. C. aceticum was Gram negative. The cells were rodshaped and peritrichously flagellated. Round spores were formed in terminal position. The DNA contained 33 mol % guanine plus cytosine. The organism was obligately anaerobic and grew either chemolithotrophically with H2+CO2 or chemoorgranotrophically with compounds such as fructose, l-glutamate, l-malate or pyruvate. H2 and CO2 were converted to acetic acid according to the following equation: $$2{\text{ CO}}_{\text{2}} + 4{\text{H}}_{\text{2}} \to {\text{CH}}_{\text{3}} {\text{COOH + 2H}}_{\text{2}} {\text{O}}$$ The optimal temperature for growth was 30°C. The optimal pH for chemolithotrophic growth was 8.3. The doubling times for chemolithotrophic and chemoorganotrophic growth were 25 and 8h, respectively.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 128 (1981), S. 294-298 
    ISSN: 1432-072X
    Keywords: Acetobacterium woodii ; Clostridium aceticum ; Mixotrophic growth ; H2 utilization ; CO2 fixation ; H2 inhibition of chemoorganotrophic growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During growth of Acetobacterium woodii on fructose, glucose or lactate in a medium containing less than 0.04% bicarbonate, molecular hydrogen was evolved up to 0.1 mol per mol of substrate. Under an H2-atmosphere growth of A. woodii with organic substrates was completely inhibited whereas under an H2/CO2-atmosphere rapid growth occurred. Under these conditions H2+CO2 and the organic substrate were utilized simultaneously indicating that A. woodii was able to grow mixotrophically. Clostridium aceticum differed from A. woodii in that H2 was only evolved in the stationary phase, that the inhibition by H2 was observed at pH 8.5 but not at pH 7.5, anf that in the presence of fructose and H2+CO2 only fructose was utilized. The hydrogenase activity of fructose-grown cells of C. aceticum amounted to only 12% of that of H2+CO2-grown cells. With A. woodii a corresponding decrease of the activity of this enzyme was not observed.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 70 (1985), S. 207-212 
    ISSN: 1432-2242
    Keywords: Pisum mutants and recombinants ; Phytotron experiments ; Gene-ecology ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The flowering behaviour of 17 Pisum mutants and 20 recombinants was studied under three different temperatures using long-day phytotron conditions. A constant low temperature of 12.5 ° C led to a strong delay in flowering in all the genotypes tested but distinct relative differences could be found between them. Relative differences were also present with regard to speed of ontogenetic development under a permanent high temperature of 25.5 °C or under an alternating change between low and high temperature. Under the low temperature, recombinants R 20D and R 20E, carrying gene efr for earliness, entered the flowering period more than 4 weeks later than the donor of efr, demonstrating thereby a negative influence of one of the other mutant genes on efr. The high temperature of 25 °C influenced the flowering behaviour of 4 fasciated genotypes negatively — in contrast to the other strains studied. The plants of recombinant R 405 produced only tiny flower buds under these conditions. None of the plants of recombinant R 142F flowered under either the constant low or high temperature — they need the change of low and higher temperature for normal flower formation. The experiments show that most of the genotypes tested react specifically to the three temperature conditions offered to them.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1988), S. 344-349 
    ISSN: 1432-2242
    Keywords: Pisum mutants and recombinants ; Phytotron experiments ; Gene-ecology ; Photoperiod ; Suppressor genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The flowering behavior of 59 Pisum mutants and 228 recombinants was studied in the phytotron in four different photoperiods (continuous light, long-day 18/6 h, short-day 12/12 h, extreme short-day 6/18 h). There was no or little difference in the response of the genotypes to long-day and permanent light, whereas great differences were observed between long- and short-day 12/12 h and between the two short-day trials. About half the genotypes tested were unable to survive or to flower in extreme short-day. Some recombinants, however, had an almost normal development under these unfavorable conditions. Gene fis controls the photoperiodic reaction of the plants: they are unable to flower in short-day. Gene fds negatively influences gene efr for earliness: it causes a strong delay of flowering of efr recombinants in long-day and suppresses the formation of functionable flowers in short-day. Most of the genotypes tested showed a specific reaction to the four photoperiods different from that of the mother variety and the other genotypes. The practical aim of our phytotron experiments is the preselection of Pisum genotypes which might be suited for cultivation in countries with short-day climate.
    Type of Medium: Electronic Resource
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