Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Purification of rosmarinic acid synthase from cell cultures of Coleus blumei Benth (1993)
    Springer
    Planta 191 (1993), S. 18-22 
    Details
    ISSN: 1432-2048
    Keywords: Coleus (cell cultures) ; Hydroxycinnamic acid ester ; Rosmarinic acid ; Rosmarinic acid synthase (purification)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rosmarinic acid synthase from cell cultures of Coleus blumei Benth. was purified to apparent homogeneity by fractionated ammonium sulfate precipitation (60–80% saturation), hydrophobic interaction chromatography, affinity chromatography and gel filtration. This purification procedure resulted in a 225-fold-enriched specific enzyme activity with a yield of 9%. The protein preparation was apparently pure according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis. The apparent molecular mass determined by gel filtration and SDS-PAGE was 77 kDa, indicating that rosmarinic acid synthase is a monomeric enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240891
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Biosynthesis and accumulation of rosmarinic acid in suspension cultures ofColeus blumei (1995)
    Springer
    Plant cell, tissue and organ culture 43 (1995), S. 89-92 
    Details
    ISSN: 1573-5044
    Keywords: biosynthesis ; caffeic acid esters ; Coleus blumei (Lamiaceae) ; plant cell cultures ; rosmarinic acid ; secondary product formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This communication reviews data on the accumulation and biosynthesis of rosmarinic acid in cell suspension cultures ofColeus blumei. The influence of the medium, mainly the carbohydrate source on growth and rosmarinic acid production in these cell cultures is described. The biosynthetic pathway of rosmarinic acid was elucidated inColeus blumei cell cultures: eight enzymatic activities are involved in the transformation of the precursors phenylalanine and tyrosine to the end product rosmarinic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00052161
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...