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  • 1
    Electronic Resource
    Electronic Resource
    l-Cysteine metabolism via 3-mercaptopyruvate pathway and sulfate formation in rat liver mitochondria (1992)
    Springer
    Amino acids 2 (1992), S. 143-155 
    Details
    ISSN: 1438-2199
    Keywords: Amino acids ; Cysteine metabolism ; 3-Mercaptopyruvate pathway ; Sulfate formation ; Mitochondria ; Glutathione
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have studied the 3-mercaptopyruvate pathway (transamination pathway) ofl-cysteine metabolism in rat liver mitochondria.l-Cysteine and other substrates at 10 mM concentration were incubated with mitochondrial fraction at pH 8.4, and sulfate and thiosulfate were determined by ion chromatography. Whenl-cysteine alone was incubated, sulfate formed was 0.7µmol per mitochondria from one g of liver per 60 min. Addition of 2-oxoglutarate and GSH resulted in more than 3-fold increase in sulfate formation, and thiosulfate was formed besides sulfate. The sum (A + 2B) of sulfate (A) and thiosulfate (B) formed was approximately 7-times that withl-cysteine alone. Incubation with 3-mercaptopyruvate resulted in sulfate and thiosulfate formation, and sulfate was formed with thiosulfate. These reactions were stimulated with glutathione. Sulfate formation froml-cysteinesulfinate and 2-oxoglutarate was not enhanced by glutathione and thiosulfate was not formed. These findings indicate thatl-cysteine was metabolized and sulfate was formed through 3-mercaptopyruvate pathway in mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00806085
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibition of sulfate-forming activity in rat liver mitochondria by (aminooxy)acetate (1993)
    Springer
    Amino acids 5 (1993), S. 245-251 
    Details
    ISSN: 1438-2199
    Keywords: Amino acids ; Cysteine metabolism ; MP pathway ; Sulfate formation ; (Aminooxy)acetate ; Rat liver mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of (aminooxy)acetate, an inhibitor of aminotransferases, on the sulfate formation froml-cysteine andl-cysteinesulfinate in rat liver mitochondria was studied. Incubation of 10 mMl-cysteine with rat liver mitochondria at 37°C in the presence of 10 mM 2-oxoglutarate and 10 mM glutathione resulted in the formation of 4.60 and 1.52µmol of sulfate and thiosulfate, respectively, per 60 min per mitochondria obtained from 1 g of liver. Under the same conditions sulfate formation froml-cysteinesulfinate was 24.96µmol, but thiosulfate was not formed. The addition of (aminooxy)acetate at 2 mM or more completely inhibited the sulfate and thiosulfate formation froml-cysteine and the sulfate formation froml-cysteinesulfinate. These findings support our previous conclusion that cysteine transamination and 3-mercaptopyruvate pathway (MP pathway) are involved in the sulfate formation froml-cysteine in rat liver mitochondria (Ubuka et al., 1992).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00805987
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Increase in tissue cysteine level and excretion of sulfate and taurine after intragastric administration ofl-2-oxothiazolidine-4-carboxylate in rats (1995)
    Springer
    Amino acids 8 (1995), S. 37-45 
    Details
    ISSN: 1438-2199
    Keywords: Amino acids ; l-2-Oxothiazolidine-4-carboxylate ; Cysteine precursor ; Cysteine metabolism ; Sulfate ; Taurine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five mmol ofl-2-oxothiazolidine-4-carboxylate (OTC)/kg of body weight was administered into the stomach of rats, and cysteine levels in tissues and sulfate and taurine excreted in the urine were determined. The cysteine (plus cystine expressed as cysteine) concentration in the liver increased to 170–200% of the original level at 30 min and that in the blood to 160% at 60 min after the OTC administration. These high levels were maintained until 8 h after the administration and decreased gradually thereafter. Excretion of sulfate and taurine increased after the OTC administration and the increase corresponded to 26% and 15%, respectively, of the OTC administered. These findings suggest that at least about 40% of the OTC administered into the stomach was taken up and converted to cysteine, which was metabolized to sulfate and taurine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00806542
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  • 4
    Electronic Resource
    Electronic Resource
    Inhibition of sulfate excretion by (aminooxy)acetate induced stimulation of taurine excretion in rats (1995)
    Springer
    Amino acids 8 (1995), S. 345-352 
    Details
    ISSN: 1438-2199
    Keywords: Amino acids ; Cysteine metabolism ; Sulfate formation ; Taurine formation ; Hypotaurine ; Sulfur equilibrium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary l-Cysteine is mainly metabolized to sulfate and taurine through cysteinesulfinate pathway. Alternatively, sulfate is formed in rat liver mitochondria via 3-mercaptopyruvate pathway. Intraperitoneal administration of 5 mmol ofl-cysteine per kg of body weight resulted in the increase in sulfate and taurine (plus hypotaurine) excretion in the 24-h urine, which corresponded to 45.3 and 29.3%, respectively, ofl-cysteine administered. Subcutaneous injection of (aminooxy)acetate, a potent inhibitor of transaminases, together withl-cysteine halved the sulfate excretion and doubled the taurine excretion. In vitro sulfate formation froml-cysteine and froml-cysteinesulfinate in rat liver mitochondria was inhibited by (aminooxy)-acetate. The sulfate-forming activity of liver mitochondria obtained from rats injected with (aminooxy) acetate was also inhibited. These results indicate that the transamination reaction is crucial in sulfate formation and in the regulation of sulfur metabolism. Sulfur equilibrium in mammals was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00806552
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  • 5
    Electronic Resource
    Electronic Resource
    Concentration of monoamines and their metabolites in the cerebrospinal fluid from patients with senile dementia of the Alzheimer type and vascular dementia of the Binswanger type (1992)
    Springer
    Journal of neural transmission 4 (1992), S. 69-77 
    Details
    ISSN: 1435-1463
    Keywords: Dopamine ; norepinephrine ; cerebrospinal fluid ; senile dementia of the Alzheimer type ; vascular dementia of the Binswanger type
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We measured the concentrations of total (conjugated and unconjugated) monoamines (dopamine, DA; norepinephrine, NE) and monoamine metabolites (homovanillic acid, HVA; 3-methoxy-4-hydroxyphenyleneglycol, MHPG; 5-hydroxyindoleacetic acid, 5-HIAA) in the cerebrospinal fluid (CSF), using HPLC-ECD in 11 patients with Alzheimer's disease (AD) or senile dementia of the Alzheimer type (SDAT), 17 patients with vascular dementia of the Binswanger type (VDBT), and 15 controls. In AD/SDAT, there was a significant decrease in the DA concentration and a significant increase in the MHPG concentration. The average NE concentration was not altered, but significantly increased with the progression of intellectual disability. There were no significant changes in HVA and 5-HIAA concentrations. Patients with VDBT showed a significant increase in the DA concentration and a significant decrease in HVA and 5-HIAA concentrations. The DA concentrations increased significantly with the progression of dementia and ventricular enlargement. These results indicate that the noradrenergic and dopaminergic system in particular are altered in AD/SDAT, while the dopaminergic and serotonergic systems are mainly involved in VDBT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02257623
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  • 6
    Electronic Resource
    Electronic Resource
    Concentration of catecholamines and indoleamines in the cerebrospinal fluid of patients with vascular parkinsonism compared to Parkinson's disease patients (1997)
    Springer
    Journal of neural transmission 104 (1997), S. 441-449 
    Details
    ISSN: 1435-1463
    Keywords: Dopamine ; L-dopa ; 3-O-methyldopa ; norepinephrine ; serotonin ; vascular parkinsonism ; Parkinson's disease ; cerebrospinal fluid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The concentration of catecholamines and indoleamines in the cerebrospinal fluid of patients with vascular parkinsonism (VP) was compared to that in patients with Parkinson's disease (PD) and controls. Compared to the controls, the concentration of tyrosine was significantly higher, and the concentration of L-dopa and 3-O-methyldopa (3-OMD) was significantly lower in both VP and PD patients. The balance between the 3-OMD/L-dopa and dopamine (DA)/L-dopa ratios was changed in favor of 3-OMD/L-dopa in both VP patients and PD patients suggesting the preservation of a compensatory mechanism. All these changes were less marked in VP patients than in PD patients. A remarkable finding was that in contrast to PD patients the concentration of DA and norepinephrine (NE) was significantly higher in VP patients than in the controls. The decrease in the concentration of 5-hydroxytryptamine (5-HT) was significantly greater in VP patients than in PD patients. In PD patients, the concentration of DA, NE, and 5-HT showed significant correlation with the severity of motor symptoms. In VP patients, the concentration of 5-HT alone showed significant correlation with the severity of motor symptoms and cognitive dysfunction. These findings suggest that VP patients may have similar disturbances in the DA synthesis pathway as PD patients, but differ from PD patients in that the concentrations of DA and NE are elevated and the decrease in the 5-HT concentration is greater in VP patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01277662
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