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  • Einzelproteine  (1)
  • Enzymurie  (1)
  • Hypertensive pregnancy  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Harnproteine als Indikatoren der diabetischen Nephropathie (1988)
    Springer
    Journal of molecular medicine 66 (1988), S. 892-898 
    Details
    ISSN: 1432-1440
    Keywords: Diabetic nephropathy ; Urine analysis ; Albumin ; Single proteins ; α-1-microglobulin ; SDS-PAGE ; Diabetische Nephropathie ; Harnanalyse ; Albumin ; Einzelproteine ; α-1-Mikroglobulin ; SDS-PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Zahlreiche Untersuchungen haben in den vergangenen 10 Jahren belegt, daß die Quantifizierung von Albumin im Urin zur frühzeitigen Diagnose der diabetischen Nephropathie geeignet ist. Dabei wurde die Albuminbestimmung aufgrund ihrer hohen Treffsicherheit bei geringem methodischen Aufwand in den Vordergrund gehoben. Die parallele Untersuchung des Harnproteinspektrums mittels SDS-Polyacrylamidgelektrophorese zeigt jedoch, daß auch andere hoch- und niedermolekulare Proteine in den verschiedenen Stadien der diabetischen Nephropathie vermehrt ausgeschieden werden, so daß eine Erweiterung der reinen Albuminbestimmung um je ein makromolekulares (z.B. Transferrin) und ein mikromolekulares (z.B. α-1-Mikroglobulin) Protein sinnvoll erscheint. Nach der vorliegenden Untersuchung können beide Verfahren (kombinierte quantitative bzw. qualitative Analyse) in der Frühdiagnostik und der Verlaufskontrolle der diabetischen Nephropathie eingesetzt werden.
    Notes: Summary During the last ten years, several studies proved the applicability of urinary albumin quantification in the early diagnosis of diabetic nephropathy. Owing to its high accuracy and its comparable low methodological effort, only the albumin determination was emphasised. Parallel studies of urinary protein patterns, however, using sodium dodecylsulfate-polyacrylamidegel-electrophoresis demonstrated the increased excretion of other high- and low-molecular mass proteins in different stages of diabetic nephropathy. Consequently an extension of the mere albumin assay including a macromolecular (e.g., transferrin) and a micromolecular (e.g. α-1-microglobulin) protein seems meaningful. According to this study, both methodological lines (combined quantitative and qualitative analysis, respectively) are useful tools in the early detection and the follow up of diabetic nephropathy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01728951
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Urinary protein electrophoresis profile in normal and hypertensive pregnancies (1989)
    Springer
    Archives of gynecology and obstetrics 246 (1989), S. 97-105 
    Details
    ISSN: 1432-0711
    Keywords: Hypertensive pregnancy ; Proteinuria ; SDS-polyacrylamide gel electrophoresis ; Western blot ; Tamm-Horsfall glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary By using of modified urine preparation and a highly sensitive SDS-polyacrylamide gel electrophoresis (SDS-PAGE) we determined the urinary protein profile in 21 healthy males, 25 healthy females, 64 patients with uncomplicated pregnancy and 110 hypertensive pregnant women. The urinary protein patterns were similar in controls and in women with a normal pregnancy. There were no increase in the number of protein bands from the 1st trimester to term, and the electrophoresis pattern did not change in the postpartum period. In both groups, an intensively stained protein band with an apparent molecular weight of 105 kD was detected. The 105 kD band was significantly reduced or completely absent in 91 (83%) out of 110 hypertensive pregnant women. The urinary protein electrophoresis profile correlated significantly with the severity of the disease. The 105 kD band disappeared just before or simultaneously with the onset of clinical symptoms in 18 out of 32 hypertensive pregnant women followed throughout pregnancy. Postpartum the 105 kD in urine reappeared at 2 to 14 days after delivery in 49 of the 53 patients. Using a silver staining and Western blot, the 105 kD band was identified as Tamm-Horsfall protein, which is identical to the immunosuppressive glycoprotein uromodulin. The findings in the SDS-PAGE may reflect a transitory tubular dysfunction in cases of pre-eclampsia, which is usually reversible after delivery. The results of our study support the hypothesis of an immunological basis for this disorder.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00934126
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mikroproteinurie und Enzymurie bei Fieber und Pyelonephritis im Kindesalter Eine prospektive Untersuchung an 180 Kindern (1997)
    Springer
    Der Urologe 36 (1997), S. 68-76 
    Details
    ISSN: 1433-0563
    Keywords: Schlüsselwörter Mikroproteinurie ; Enzymurie ; Fieber ; Pyelonephritis ; Kinder ; Key words Microproteinuria ; Enzymuria ; Fever ; Pyelonephritis ; Childhood
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In 180 children (87 children belonging to a control group, 68 with fever of non-renal origin, and 25 with pyelonephritis) albumin and immunoglobulin G (markers for glomerular dysfunction), α-1-microglobulin and β -NAG (markers for proximal tubular dysfunction) and apolipoprotein A1 (marker of ’postrenal' dysfunction) were measured in second-voided morning urine. In children with fever of non-renal origin, glomerular dysfunction was encountered in 8.8 %, tubular dysfunction in 17.6 % and mixed glomerular-tubular dysfunction in 14.7 % of cases. Among children with pyelonephritis, 28 % revealed glomerular dysfunction and 44 % mixed glomerular-tubular dysfunction. No case of solitary proximal tubular dysfunction was observed in children with pyelonephritis. There were highly significant differences in presence and expression of glomerular dysfunction between children with fever of non-renal origin and children with pyelonephritis (P 〈 0.0001), whereas with regard to proximal tubular dysfunction, the differences were only moderately significant (β -NAG: P 〈 0.01) or of low significance (α-1-microglobulin: P 〈 0.05). This may indicate that morphologic changes occur during interstitial pyelonephritis due to inflammation of glomeruli, resulting in glomerular dysfunction, while proximal tubular dysfunction may additionally be due to fever-associated function processes.
    Notes: Zusammenfassung Bei 180 Kindern (87 Kinder einer Kontrollgruppe, 68 Kinder mit Fieber nicht-renaler Genese, 25 Kinder mit Pyelonephritis) wurden Albumin und Immunglobulin G (glomeruläre Funktionsparameter), α-1-Mikroglobulin und N-Acetyl-β-D-Glucosaminidase (β-NAG); Funktionsparameter des proximalen Nierentubulus) sowie Apolipoprotein A1 (''postrenaler’ Funktionsparameter) im 2. Morgenurin gemessen. Bei Kindern mit Fieber nicht-renaler Genese fanden sich dabei in 8,8 % rein glomeruläre, in 17,6 % rein tubuläre, in 14,7 % gemischt glomerulär-tubuläre Funktionsstörungen. Bei Pyelonephritis zeigten dagegen 28 % der Kinder rein glomeruläre, 44 % der Kinder gemischt glomerulär-tubuläre Funktionsbeeinträchtigungen. Reine Funktionsstörungen des proximalen Tubulussystems wurden bei Pyelonephritis nicht beobachtet. Hinsichtlich der glomerulären Funktionsparameter bestanden hochsignifikante Unterschiede (p 〈 0,0001) zwischen Kindern mit Fieber nicht-renaler Genese und Kindern mit Pyelonephritis, während für die Parameter des proximalen Tubulussystems lediglich signifikante (β-NAG: p 〈 0,01) bzw. nur schwach signifikante (α-1-Mikroglobulin: p 〈 0,05) Unterschiede beobachtet werden konnten. Diese Ergebnisse deuten darauf hin, daß glomeruläre Funktionsstörungen bei der interstitiellen Pyelonephritis vorliegen und möglicherweise Folge einer morphologischen Mitbeteiligung von Nierenglomerula sind, während Funktionsstörungen des proximalen Tubulussystems durch funktionelle fieberassoziierte Prozesse bedingt sein könnten.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001200050069
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    Paper (German National Licenses)
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