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  • Monoclonal antibodies  (2)
  • Epithelial membrane antigen  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Non-specific binding of mouse myeloma IgM immunoglobulins by human myelin sheaths and astrocytes (1986)
    Springer
    Acta neuropathologica 70 (1986), S. 284-288 
    Details
    ISSN: 1432-0533
    Keywords: Myelin sheaths ; Astroglial cells ; Mouse IgM ; GFAP ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The immunoreactivity of purified mouse myeloma IgM immunoglobulins (mouse IgM) to human myelin sheaths and astroglial cells was evaluated with the peroxidase-antiperoxidase method on paraffin-embedded tissues from human gliomas and areas of multiple sclerosis, and from normal human cerebrum, spinal cord and spinal nerve roots. The mouse IgM reacted positively with central and peripheral myelin sheaths and, as shown independently by others, with the cytoplasm of neoplastic and reactive astroglia. Parallel immunostaining of successive sections with an anti-glial fibrillary acidic protein (GFAP) serum and/or the anti-Leu 7 monoclonal antibody was of considerable assistance in identifying the immunoreactive elements and in distinguishing specific from non-specific immunostaining of myelin sheaths and astroglia. Pretreatment with normal human serum inhibited the non-specific binding by mouse IgM without altering GFAP and Leu 7 reactivities. The non-specific binding of mouse IgM to human myelin sheaths and astroglia can therefore be overcome, and the specificity of mouse IgM monoclonal antibodies retained, by the parallel immunostaining of successive sections with mouse IgM. If nonspecific binding by mouse IgM is found to occur, it can then be inhibited by preincubation with normal human serum without loss of specific antigenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686085
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Retinal S-antigen immunoreactivity in medulloblastomas (1988)
    Springer
    Acta neuropathologica 76 (1988), S. 204-207 
    Details
    ISSN: 1432-0533
    Keywords: S-Antigen ; Monoclonal antibodies ; Cerebellar medulloblastoma ; Photoreceptor cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of 16 cerebellar medulloblastomas were studied immunohistochemically using a four-step immunoperoxidase (PAP) method and a monoclonal antibody (MAbA9-C6) which defines an epitope of the retinal S-antigen, a protein known to occur in retinal photoreceptor cells and pinealocytes of the pineal gland as well as in retinoblastomas, pineocytomas and pineoblastomas. Immunopositivity was demonstrated in a variable number of tumor cells in 50% of the cases. This finding may be an indication of a differentiation potential of medulloblastomas along the photoreceptor cell lineage. Alternatively, it may simply indicate the non-specificity of the retinal antigen in the neoplastic state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688104
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Expression of epithelial membrane antigen in perineurial cells and their derivatives (1987)
    Springer
    Acta neuropathologica 75 (1987), S. 160-165 
    Details
    ISSN: 1432-0533
    Keywords: Epithelial membrane antigen ; Pacinian corpuscles ; Perineurial cells ; Perineurioma ; Neurothekeoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Paraffin-embedded surgical pathology specimens from skin (5) and muscle (2) biopsies, from Morton's neuromas (3), traumatic neuromas (8), schwannomas (21), neurofibromas (12), and from one perineurioma and one neurothekeoma were studied by immunoperoxidase histochemistry and antibodies against epithelial membrane antigen (EMA), Leu 7 epitopes (Leu 7), S-100 protein (S-100) and cytokeratins. Normal, reactive and neoplastic perineurial cells stain consistently for EMA, whereas Schwann cells express Leu 7 and/or S-100 positivity. None of the immunoreactive cells stained for cytokeratin. Our findings indicate that perineurial and Schwann cells can easily be distinguished by their different patterns of immunoreactivity with the above markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687077
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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