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  • 1
    Electronic Resource
    Electronic Resource
    Histochemical methods for the demonstration of thomsen-friedenreich antigen in cell suspensions and tissue sections (1978)
    Springer
    Journal of molecular medicine 56 (1978), S. 761-765 
    Details
    ISSN: 1432-1440
    Keywords: Thomsen-Friedenreich Antigen ; Erdnußagglutinin ; Neuraminidase ; Autoradiographie ; Fluorescenzmikroskop ie ; Thomsen-Friedenreich antigen ; Peanut agglutinin ; Neuraminidase ; Autoradiography ; Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary 1. Three different methods are described for the visualisation of the Thomsen-Friedenreich (TF) antigen on cell suspensions, formalin-fixed and paraffin embedded or frozen tissue sections: a) Rosette formation with chicken and sheep erythrocytes. b) Fluorescence-microscopy with fluorescein labelled peanut agglutinin. c) Autoradiography with3H-labelled peanut agglutinin. 2. The TF antigen was shown, as far as presently investigated, to be exposed on various blood cells, glomerula of the kidney and normal mammary gland after neuraminidase treatment. Mammary gland was also shown to possess TF receptors without prior treatment with neuraminidase. 3. The exposure of this cryptantigen can be brought about by bacterial or viral neuraminidase and is followed by an antigen/antibody reaction, which can lead to possible pathological consequences.
    Notes: Zusammenfassung 1. Drei verschiedene Methoden zur Darstellung von sog. Thomsen-Friedenreich (TF)-Antigenen in Zellsuspensionen, an Formalin-fixiertem und in Paraffin eingebettetem Gewebe sowie in Gefrierschnitten werden beschrieben: a) Rosetten-Bildung mit Hühner- und Schaferythrocyten. b) Fluorescenzmikroskopie mit Fluorescein-markiertem Erdnußagglutinin. c) Autoradiographie mit3H-markiertem Erdnußagglutinin. 2. Nach Neuraminidasebehandlung konnten TF-Antigene — soweit bisher untersucht — auf verschiedenen Blutzellen, in Nierenglomerula und in normalem Brustdrüsengewebe nachgewiesen werden. Ein Teil derartiger Antigene lag im Brustdrüsenparenchym auch bereits als freie Rezeptoren vor, d. h. waren nicht von Neuraminsäure bedeckt. 3. Die Freilegung dieser Kryptantigene kann im Rahmen von Infektionen durch bakterielle und virale Neuraminidase erfolgen. Dabei kommt es zu einer Antigen/Antikörper Reaktion mit unter Umständen klinisch relevanten Folgen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01476765
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  • 2
    Electronic Resource
    Electronic Resource
    Experimental investigations on a sequential combination chemotherapy protocol (1980)
    Springer
    Journal of cancer research and clinical oncology 96 (1980), S. 65-78 
    Details
    ISSN: 1432-1335
    Keywords: Ehrlich ascites tumor ; Proliferation kinetics ; Partial synchronization ; Combination chemotherapy ; Cytostatic drugs ; Adriamycin ; Hydroxyurea ; Vincristine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This paper deals with experimental investigations concerning the composition of a cytostatic three-drug-protocol in diploid Ehrlich-Ascites-Tumor (EAT) cells in vivo at a far advanced stage of the disease. Hydroxyurea (HU) and vincristine (VCR) were used in very low doses to induce a modification of the growth pattern of tumor cells alike partial synchronization. Adriamycin (ADM) was selected as cytocidal drug during DNA synthesis of the partially synchronized cells. It was found that the sequential combination of HU and VCR (first HU and 12h thereafter VCR) caused the greatest alteration of growth pattern compared with other combination protocols. A further statistically significant increase of the degree of synchrony was observed after a second VCR administration—22 h after HU. By means of this protocol the EAT was subdivided into two proliferating subpopulations, a diploid and a tetraploid one. the tetraploid population resulted from surviving cells being not able to perform cytokinesis correctly, so that polynuclear cells and cells with a large single nucleus containing tetraploid DNA values were created. With respect to therapy, the administration of ADM at the time of DNA synthesis of the partially synchronized cells resulted in a statistically significant prolongation of the mean survival time and in 30% of cures of the animals. The dosage of ADM was 2.6 mg/kg, i.e., a nonlethal dose (50% of the LD10). Other combinations, i.e., simultaneous or reversed sequential combinations, did not show any therapeutic improvement compared to single drug therapy of ADM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412898
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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