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  • Inorganic Chemistry  (8)
  • Theoretical, Physical and Computational Chemistry  (7)
  • Immunocytochemistry  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Regeneration and post-metamorphic development of the central nervous system in the protochordate Ciona intestinalis: a study with monoclonal antibodies (1995)
    Springer
    Cell & tissue research 279 (1995), S. 421-432 
    Details
    ISSN: 1432-0878
    Keywords: Trans-differentiation ; Proliferation ; Bromodeoxyuridine ; Immunocytochemistry ; Regeneration ; Ciona intestinalis (Tunicata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In this study, we use three monoclonal antibodies that recognise antigens present in the central nervous system of the ascidian Ciona intestinalis to study regeneration and post-metamorphic development of the neural ganglion. We have also used bromodeoxyuridine labelling to study generation of the neuronal precursor cells. The first antibody, CiN 1, recognises all neurones in the ganglion, whereas the second, CiN 2, recognises only a subpopulation of the large cortical neurones. Western blotting studies show that CiN 2 recognises two membrane-bound glycoproteins of apparent Mr 129 and 100 kDa. CiN 1 is not reactive on Western blots. Immunocytochemical studies with these antibodies show that CiN 1-immunoreactive neurone-like cells are present at the site of regeneration as early as 5–7 days post-ablation, a sub-population of CiN 2-immunoreactive cells being detected by 9–12 days post-ablation. The third antibody, ECM 1, stains extracellular matrix components and recognises two diffuse bands on Western blots of whole-body and ganglion homogenates. The temporal and spatial pattern of appearance of CiN 1 and CiN 2 immunoreactivity both during post-metamorphic development and in regeneration occurs in the same sequence in both processes. Studies with bromodeoxyuridine show labelled nuclei in some neurones in the regenerating ganglion. Plausibly these originate from the dorsal strand, an epithelial tube that reforms by cell proliferation during the initial phases of regeneration. A second population of cells, the large cortical neurones, do not incorporate bromodeoxyuridine and thus must have been born prior to the onset of regeneration. This latter finding indicates a mechanism involving trans-differentiation of other cell types or differentiation of long-lived totipotent stem cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318500
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  • 2
    Electronic Resource
    Electronic Resource
    Localisation of gamma aminobutyric acid (GABA)-like immunoreactivity in the echinoderm Asterias rubens (1994)
    Springer
    Cell & tissue research 278 (1994), S. 177-185 
    Details
    ISSN: 1432-0878
    Keywords: Key words: GABA ; Immunocytochemistry ; Radial nerve cord ; Tube feet ; Digestive system ; Asterias rubens (Echinodermata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Gamma amino butyric acid (GABA) is believed to be the principal inhibitory neurotransmitter in the mammalian central nervous system, a function that has been extended to a number of invertebrate systems. We have used a specific antiserum raised against GABA to demonstrate GABA-like immunoreactivity in the radial nerve cord (RNC), tube feet and the digestive system of the asteroid Asterias rubens. In the RNC, immunoreactivity was restricted to ectoneural fibres and cell bodies while in the tube feet fibres were revealed in the basal nerve ring and longitudinal nerve. In the gut, extensive labelling was apparent in the basi-epithelial plexus as well as in mucosal perikarya.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305790
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical mapping of the novel echinoderm neuropeptide SALMFamide 1 (S1) in the starfish Asterias rubens (1993)
    Springer
    Cell & tissue research 274 (1993), S. 605-618 
    Details
    ISSN: 1432-0878
    Keywords: SALMFamide ; Neuropeptide ; Immunocytochemistry ; Mapping ; Asterias rubens (Echinodermata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The recent isolation and characterization of the SALMFamide neuropeptides S1 and S2 from the starfish Asterias rubens has initiated a series of studies on their distribution. Specific antisera have been raised against S1 and used in light-microscopical immunocytochemistry. The results of this study reveal for the first time a possible hyponeural innervation of the visceral musculature of the gut and the widespread neuronal distribution of S1, (i) in axons and cell bodies of both ectoneural and hyponeral regions of the radial nerve cord and circumoral nerve ring, (ii) in the nerve ring and nerve plexus of the tube feet, (iii) in the apical muscle, (iv) in skin, and (v) extensively throughout the digestive system. These discoveries are of particular interest in terms of the possible functional roles for S1 in Asterias rubens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314559
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  • 4
    Electronic Resource
    Electronic Resource
    Regeneration and post-metamorphic development of the central nervous system in the protochordate Ciona intestinalis: a study with monoclonal antibodies (1995)
    Springer
    Cell & tissue research 279 (1995), S. 421-432 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Trans-differentiation ; Proliferation ; Bromodeoxyuridine ; Immunocytochemistry ; Regeneration ; Ciona intestinalis (Tunicata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In this study, we use three monoclonal antibodies that recognise antigens present in the central nervous system of the ascidian Ciona intestinalis to study regeneration and post-metamorphic development of the neural ganglion. We have also used bromodeoxyuridine labelling to study generation of the neuronal precursor cells. The first antibody, CiN 1, recognises all neurones in the ganglion, whereas the second, CiN 2, recognises only a subpopulation of the large cortical neurones. Western blotting studies show that CiN 2 recognises two membrane-bound glycoproteins of apparent Mr 129 and 100 kDa. CiN 1 is not reactive on Western blots. Immunocytochemical studies with these antibodies show that CiN 1-immunoreactive neurone-like cells are present at the site of regeneration as early as 5–7 days post-ablation, a sub-population of CiN 2-immunoreactive cells being detected by 9–12 days post-ablation. The third antibody, ECM 1, stains extracellular matrix components and recognises two diffuse bands on Western blots of whole-body and ganglion homogenates. The temporal and spatial pattern of appearance of CiN 1 and CiN 2 immunoreactivity both during post-metamorphic development and in regeneration occurs in the same sequence in both processes. Studies with bromodeoxyuridine show labelled nuclei in some neurones in the regenerating ganglion. Plausibly these originate from the dorsal strand, an epithelial tube that reforms by cell proliferation during the initial phases of regeneration. A second population of cells, the large cortical neurones, do not incorporate bromodeoxyuridine and thus must have been born prior to the onset of regeneration. This latter finding indicates a mechanism involving trans-differentiation of other cell types or differentiation of long-lived totipotent stem cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050299
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Localisation of gamma aminobutyric acid (GABA)-like immunoreactivity in the echinoderm Asterias rubens (1994)
    Springer
    Cell & tissue research 278 (1994), S. 177-185 
    Details
    ISSN: 1432-0878
    Keywords: GABA ; Immunocytochemistry ; Radial nerve cord ; Tube feet ; Digestive system ; Asterias rubens (Echinodermata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Gamma amino butyric acid (GABA) is believed to be the principal inhibitory neurotransmitter in the mammalian central nervous system, a function that has been extended to a number of invertebrate systems. We have used a specific antiserum raised against GABA to demonstrate GABA-like immunoreactivity in the radial nerve cord (RNC), tube feet and the digestive system of the asteroid Asterias rubens. In the RNC, immunoreactivity was restricted to ectoneural fibres and cell bodies while in the tube feet fibres were revealed in the basal nerve ring and longitudinal nerve. In the gut, extensive labelling was apparent in the basi-epithelial plexus as well as in mucosal perikarya.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305790
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Darstellung und physikalische Eigenschaften mesoionischer 1,3-Dithiolone1) (1976)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 109 (1976), S. 740-752 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Preparation and Physical Properties of Mesoionic 1,3-Dithiolones1)1,3-Dithiolylium-4-olates 3a-1 are preparatively interesting mesoionic 6π Arenes. Their synthesis from the corresponding dithioester derivatives 1a-1 and their infrared, electronic, and mass spectra are described. 3a, b, and f possess as sydnone analogues compounds relatively high dipole moments of 5.00, 5.54, and 5.98 D, respectively.
    Notes: 1,3-Dithiolylium-4-olate 3a-1 sind präparativ interessante mesoionische 6π-Aromaten. Ihre Synthese aus den entsprechenden Dithiocarbonester-Derivaten 1a-1 sowie Infrarot-, Elektronen- und Massenspektren werden beschrieben. Als Sydnon-Analoga besitzen 3a, b und f relativ große elektrische Momente von 5.00, 5.54 bzw. 5.98 D.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19761090235
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  • 7
    Electronic Resource
    Electronic Resource
    The electronic structure of phosphorus cages with the nortricyclane skeleton. - Model calculations and photoelectron spectroscopic investigations (1983)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 116 (1983), S. 2972-2982 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Die Elektronenstruktur von Phosphor-Verbindungen mit Nortricyclan-Struktur. - Modell-Rechnungen und PhotoelektronenspektrenDie unterschiedliche Reaktivität der Phosphor-Verbindungen P4[Si(CH3)2]3 (1) and P7(CH3)3 (2) wird anhand eines einfachen MO-Schemas diskutiert. Das MO-Schema basiert auf semiempirischen Rechnungen (MINDO/3, MNDO) und auf He(1)-photoelektronenspektroskopischen Untersuchungen an 1 und 2. Die Elektronenstruktur ähnlich gebauter Käfigverbindungen mit Nortricyclangerüst wie P73-, P4S3 und P7[Si(CH3)3]3 wird mit 1 und 2 verglichen.
    Notes: The different reactivity of P4[Si(CH3)2]3 (1) and P7(CH3)3 (2) is discussed in terms of a simple MO scheme. This scheme is based on semiempirical calculations (MINDO/3, MNDO) and on He(1) photoelectron spectroscopic investigations on 1 and 2. The electronic structure of similar cage compounds with the nortricyclane skeleton like P73-, P4S3, and P7[Si(CH3)3]3 is compared with 1 and 2.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19831160823
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  • 8
    Electronic Resource
    Electronic Resource
    Die Elektronenstruktur und das He(I)-Photoelektronen-Spektrum von (Cyclopentadienyl)(1,4-dimethyl-1,4-dibora-2,5-cyclohexadien)cobalt (1982)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 115 (1982), S. 754-763 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: The Electronic Structure and the He(I) Photoelectron Spectrum of (Cyclopentadienyl)(1,4-dimethyl-1,4-dibora-2,5-cyclohexadiene)cobaltThe electronic structure of (cyclopentadienyl)(1,4-dimethyl-1,4-dibora-2,5-cyclohexadiene)cobalt (1) has been investigated by means of semiempirical INDO calculations and by means of the He(I) photoelectron (PE) spectroscopy. The cyclopentadienyl ligand is bonded due to Co 3dyz to the transition metal center, while the dibora system is coupled via 3dxz to the Co atom. In the electronic groundstate several ligand orbitals are predicted on top of the Co 3d valence orbitals. Strong reorganization effects of the Co 3d levels during the ionization cause a switch of the groundstate orbital sequence. The first PE bands are due to Co 3d orbitals. By means of perturbational calculations based on the Green's function approach it is possible to reproduce the experimental PE spectrum with sufficient accuracy.
    Notes: Die Elektronenstruktur von (Cyclopentadienyl)(1,4-dimethyl-1,4-dibora-2,5-cyclohexadien)cobalt (1) wurde mit Hilfe von semiempirischen INDO-Rechnungen sowie He(I)-Photoelektronen(PE)-Spektrum untersucht. Der Cyclopentadienyl-Ligand wird durch Co-3dyz an das Zentralatom gebunden, während das Dibor-System über 3dxy an Co gekoppelt wird. Im elektronischen Grundzustand liegen mehrere Ligandenniveaus oberhalb der Co-3d-Valenzorbitale. Bei der Ionisation führen starke Reorganisationseffekte innerhalb der Co-3d-Orbitale zu einer Vertauschung der Orbitalsequenz des Grundzustands; die ersten PE-Banden werden durch Co-3d-Niveaus hervorgerufen. Durch störungstheoretische Rechnungen, die auf dem Formalismus der Greenschen Funktion beruhen, läßt sich das gemessene PE-Spektrum theoretisch gut reproduzieren.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19821150236
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  • 9
    Electronic Resource
    Electronic Resource
    Ein neuer präparativer Zugang zu Thiophen-Derivaten durch thermische [3 + 2]-Cycloadditionen mesoionischer 1,3-Dithiolone and Alkine1) (1976)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 109 (1976), S. 753-760 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: A New Preparative Entry to Thiophene Derivatives by Way of Thermal [3 + 2] Cycloaddition Reactions of Mesoionic 1,3-Dithiolones to AlkynesAs cyclic thiocarbonyl ylids the 1,3-dithiolylium-4-olats 1a-jcombine in 2,5-position at 90-130°C with dimethyl acetylenedicarboxylate with formation of non-isolable primary adducts of type 2. The latter fragment with fast evolution of carbon oxisulfide to give the thiophene diesters 3a, b, d, f, h-j in 67-100% yield. The analogous cycloaddition reactions of 1a with propiolic ester, phenylpropiolic ester, acetyl phenylacetylene, benzoyl phenylacetylene and phenylacetylene yield the thiophene derivatives 6-10.
    Notes: Die 1,3-Dithiolylium-4-olate 1a-j nehmen als cyclische Thiocarbonyl-ylide in 2.5-Stellung bei 90-130°C Acetylendicarbonester unter Bildung nicht isolierbarer Primäraddukte vom Typ 2 auf. Diese zerfallen rasch unter Kohlenoxysulfid-Abspaltung in 67-100proz. Ausbeute zu den Thiophen-dicarbonestern 3a, b, d, f, h-j. Die analogen Cycloadditionen von 1a an Propiolester, Phenylpropiolester, Acetylphenylacetylen, Benzolylphenylacetylen bzw. Phenylacetylen ergeben die Thiophen-Derivate 6-10.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19761090236
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  • 10
    Electronic Resource
    Electronic Resource
    Die He(I)-Photoelektronenspektren von Tetracarbonyleisen(0)-Komplexen mit Carbenen (1980)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 113 (1980), S. 3629-3646 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: The He(I) Photoelectron Spectra of Tetracarbonyliron(l0) Complexes with CarbenesThe He(I) photoelectron(PE) spectra of the tetracarbonyl(carbene)iron(0) complexes 1-3 have been recorded. The first six bands were assigned by calculating the different ionic states separately (ΔSCF procedure) or by using the „Transition Operator Model“. It is found that the Koopmans′ defects for the orbitals localized at the iron are larger than for orbitals localized at the ligands.
    Notes: Die He(I)-Photoelektronen(PE)-Spektren der Tetracarbonyl(carben)eisen(0)-Komplexe 1-3 wurden gemessen. Die Zuordnung der ersten sechs Banden basiert auf der Berechnung der verschiedenen Kationenzustände mittels der ΔSCF-Methode und mit Hilfe des „Transition Operator“-Modells. Für die Orbitale, die hauptsächlich am Metall lokalisiert sind, werden größere Koopmans-Defekte gefunden als für die an den Liganden lokalisierten Orbitale.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19801131121
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