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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 65 (1983), S. 145-147 
    ISSN: 1432-2242
    Keywords: Diploid wheat ; Nucleolus organiser ; In situ hybridisation ; Telocentric markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The two nucleolus organiser chromosomes of diploid wheat are identified as 1A and 5A by the combination of in situ hybridisation and cytological markers.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 58 (1980), S. 97-100 
    ISSN: 1432-2242
    Keywords: Nucleolus organizers ; In situ hybridisation ; Diploid wheats ; Ribosomal RNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Labelled RNA, transcribed in vitro from wheat ribosomal DNA cloned in a bacterial plasmid, has been hybridised to metaphase chromosomes of five diploid wheats. Autoradiography of the chromosomes has provided unequivocal evidence that these genotypes possess two pairs of nucleolus organizer chromosomes. The diploid wheat accessions used possess widely differing numbers of ribosomal RNA genes.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Triticum timopheevi ; Chromosomes ; C-banding ; In situ hybridisation ; Heterochromatin ; Translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromosomes of the tetraploid wheats Triticum timopheevi (Genome AAGG) and T. araraticum (Genome AAGG) were C-banded at mitosis. The identity of the banded and unbanded chromosomes was then established by firstly making comparisons with the hexaploid species T. zhukovskyi which has the genome formula AAAAGG. Secondly, the meiotic pairing in F1 hybrids between T. timopheevi and diploid wheats was examined by means of C-banding. The results showed that the banded chromosomes belonged to the G genome, while the unbanded chromosomes belonged to the A genome. Only one of the two pairs of satellited chromosomes had strong heterochromatic bands. The relationship between the genomes of T. timopheevi and T. dicoccum (Genome AABB) was then assessed at meiosis in hybrids between these species, using the techniques of C-banding and in situ hybridisation of a cloned ribosomal RNA gene probe. It was concluded that there were differences both in the amount and distribution of heterochromatin and also translocation differences between the species.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 61 (1982), S. 285-288 
    ISSN: 1432-2242
    Keywords: Nucleolar organisers ; In situ hybridisation ; Tetraploid and hexaploid wheats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The technique of in situ hybridisation of cloned ribosomal DNA has been used to establish the numbers of nucleolar organising sites in a range of tetraploid and hexaploid wheats.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5060
    Keywords: Aneuploidy ; dwarfing genes ; Triticum aestivum ; preferential transmission ; Aegilops sharonensis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4S l from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4S l L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5060
    Keywords: alien introduction ; chromosome pairing ; fluorescent in situ hybridization ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fluorescent in situ hybridization (FISH) of DNA to plant chromosomes has proved to be a powerful cytogenetic tool. The value of fluorescent in situ hybridization of total genomic DNA (GISH) of related species is demonstrated in the determination of wheat/alien chromosome pairing in hybrids. Its use for assessing the relative merits of the various genes that affect chromosome pairing is also shown. The ability of GISH to identify the presence in wheat of whole alien chromosomes or alien chromosome segments is illustrated. The potential of FISH for detecting repeated DNA sequences, low copy sequences and single copy genes is discussed.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5060
    Keywords: alien introduction ; fluorescent in situ hybridization (FISH) ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fluorescent in situ hybridization (FISH) has been used to assess the occurrence and frequency of wheat-alien chromosome pairing in a wheat/Thinopyrum bessarabicum hybrid and in wheat/rye hybrids with different levels of chromosome pairing by examining pollen mother cells at metaphase I of meiosis. The use of FISH to identify the presence and size of alien chromatin in a wheat background is also demonstrated. The value of FISH as an aid to the introgression of alien genetic variation into wheat is discussed.
    Type of Medium: Electronic Resource
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