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Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chondrocytes (1997)

Fukuda, K. ; Takayama, M. ; Ueno, M. ; [et al.]

Springer

Inflammation research 46 (1997), S. 114-117

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ISSN: 1420-908X

Keywords: Key words: Cartilage — Arthritis — Hyaluronic acid — Superoxide anion — Interleukin 1

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract. Objective: To examine the effect of hyaluronic acid (HA) on the induction of superoxide anion by IL-1 in chondrocytes. ¶Materials and Methods: Bovine articular chondrocytes were treated with different concentrations of IL-1. A chemiluminescent probe (L-012) was added to the medium and chemiluminescence detection was used to measure superoxide anion. ¶Results: IL-1 caused induction of superoxide anions in a dose-dependent manner. HA (10–100 〈mu〉g/ml) countered superoxide induction caused by 20 ng/ml of IL-1. ¶Conclusion: HA can afford protection against cartilage degradation, probably acting as a free-radical scavenger.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s000110050132

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Importance of spore mutants for fed-batch and continuous fermentation of Bacillus subtilis (1995)

Oh, M. K. ; Kim, B. G. ; Park, S. H.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 47 (1995), S. 696-702

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ISSN: 0006-3592

Keywords: Bacillis subtilis ; spore mutant ; fed-batch ; continuous culture ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make it possible to identify the peculiar characteristics of the spore mutants unobtainable in batch culture. Spore mutants can enhance subtilisin productivity and prolong subtilisin production time in fed-batch culture as well as enable us to use very low dilution rates (〈0.1 h-1) without losing productivity in continuous culture, thereby improving the conversion yield of the nitrogen source. At 0.05 h-1 the spollG mutant of Bacillus subtilis DB104 (Δnpr Δapr) (Emr) spollG (Bimr):: pMK101 (Cmr) showed a subtilisin yield about ten times higher than that from wild-type DB104 (Δnpr Δapr)::pMK101 (Cmr). © 1995 John Wiley & Sons, Inc.

Additional Material: 2 Ill.