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  • 1
    Electronic Resource
    Electronic Resource
    Increased migration of neutrophils to granulocyte-colony stimulating factor in rat carrageenin-induced pleurisy: Roles of complement, bradykinin, and inducible cyclooxygenase-2 (1996)
    Springer
    Inflammation research 45 (1996), S. 335-346 
    Details
    ISSN: 1420-908X
    Keywords: Granulocyte colony-stimulating factor ; Neutrophil migration ; Complement ; Kinin ; Cyclo-oxygenase-2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Administration of human recombinant granulocyte colony-stimulating factor (G-CSF, 100 μg/kg/day, s.c) to rats for 4 days significantly increased circulating neutrophil counts (by 1130%), together with an increase in mononuclear leukocyte counts (by 119%). Infiltrated pleural neutrophil counts in G-CSF-treated rats (G-CSF-r) 5h after the intrapleural injection of zymosanactivated serum were significantly higher (by 155%) than those in control rats (Vehicle-r). In carrageenin-induced pleurisy, counts of infiltrated pleural neutrophils in G-CSF-r 5 and 7h after carrageenin were significantly higher (by 119% and 116%) than those in Vehicle-r. G-CSF treatment increased the volume of pleural exudate and the plasma exudation rate by 122% and 226%, compared to values in Vehicle-r 5h after carrageenin. Cobra venom factor (75 μg/kg, i.v.) significantly reduced pleural neutrophil migration in G-CSF-r (by 53%) and Vehicle-r (by 49%). Bromelain (10 mg/kg, i.v.) and aspirin (100 mg/kg, p.o.) reduced pleural neutrophil migration and reduced exudate volume and plasma exudation. Intrapleural bradykinin-(1–5) and prostaglandin E2 levels were significantly higher in G-CSF-r than in Vehicle-r. The increased neutrophil migration in G-CSF-r may be atributed to enhanced activation of the complement system facilitated by increased plasma exudation due to bradykinin and prostaglandins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02252946
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of bradykinin-(1–5) in inflammatory exudate by a new ELISA as a reliable indicator of bradykinin generation (1996)
    Springer
    Inflammation research 45 (1996), S. 416-423 
    Details
    ISSN: 1420-908X
    Keywords: Enzyme-linked immunosorbent assay (ELISA) ; Bradykinin-(1–5) ; Carageenin-induced pleurisy ; Nasal allergy ; Kinin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have developed an ELISA for BK-(1–5) (Arg1-Pro2-Pro3-Gly4-Phe5). In rat carrageenin-induced pleurisy, in which a plasma exudation peak was observed 5 h after carrageenin, BK levels in the exudates were negligible (〈60 pg/rat). BK-(1–7) (des-Phe8-Arg9-BK) was detectable (900–400 pg/rat) over the entire course of the inflammation. However, a larger amount of BK-(1–5) was detectable in association with the increase in plasma exudation, showing a peak (8800±1200 pg/rat) 3 h after carrageenin. Bromelain (10 mg/kg, i.v.) and soy bean trypsin inhibitor (0.3 mg/rat, intra-pleural) significantly reduced BK-(1–5) levels (by 60–93%, 3, 7 and 19 h after carrageenin) and plasma exudation rates (by 61–74%, 3 and 7 h after carrageenin). Dexamethasone (0.3 mg/kg, i.p.) reduced BK-(1–5) levels (by 78%) and decreased plasma exudation (by 70%) 3 h after carrageenin. In nasal allergy patients, antigen challenge of nasal mucosa elevated BK-(1–5) levels and active kallikrein levels in nasal washes. These results verify that BK-(1–5) determined by ELISA is a good indicator for release of kinins in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02252938
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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