ISSN:
1432-0983
Keywords:
Yeast
;
Rapid transformation
;
Cell age
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00317931
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