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  • 1
    Electronic Resource
    Electronic Resource
    Recent Insights on DNA Repair: The Mechanism of Damaged Nucleotide Excision in Eukaryotes and Its Relationship to Other Cellular Processes (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 726 (1994), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1994.tb52829.x
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  • 2
    Electronic Resource
    Electronic Resource
    Interactions among genes controlling sensitivity to radiation (RAD) and to alkylation by nitrogen mustard (SNM) in yeast (1982)
    Springer
    Current genetics 5 (1982), S. 33-38 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Multiple mutants of DNA repair ; Sensitivity to nitrogen mustard and to radiation ; Thermoconditional DNA repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three haploid yeast mutants (snm) sensitive or thermoconditionally sensitive to the DNA cross-linking agent nitrogen mustard (HN2) were crossed with four rad strains representing mutations in the three pathways of DNA dark repair. The resulting haploid double and triple mutant strains were tested for their sensitivity to UV, HN2 and HN1. From the observed epistatic or synergistic interactions of the combinations of mutant alleles we could derive the relation of the SNM1 and SNM2 genes to the postulated repair pathways. Alleles snm1-1 and snml-2 ts were found epistatic to genes of the rad3 group, whereas snm2-1 ts was epistatic to rad6. The snm1 and snm2 mutant alleles interacted synergistically. From these data it is concluded that the SNM1 gene product plays a cross-link specific role in excision repair while the SNM2 gene product may be involved in a system of error-prone repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445738
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae (1986)
    Springer
    Current genetics 10 (1986), S. 871-878 
    Details
    ISSN: 1432-0983
    Keywords: UV-mutagenesis in yeast ; Thermoconditional repair mutant ; Excision repair ; DNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2 ts ) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin) was determined. Additionally, an analysis of the dose-response pattern of mutation induction (“mutation kinetics”) at several ochre alleles was carried out. It was concluded that the UV-inducible REV2 dependent mutagenic repair process is not induced in excision-deficient cells. In excision-deficient cells, REV2 dependent mutation fixation is slow and mostly postreplicative though not dependent on DNA replication. The REV2 mediated mutagenic process could be separated from the repair function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398283
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  • 4
    Electronic Resource
    Electronic Resource
    DNA repair genes of Saccharomyces cerevisiae: complementing rad4 and rev2 mutations by plasmids which cannot be propagated in Escherichia coli (1986)
    Springer
    Current genetics 11 (1986), S. 205-210 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Excision repair ; Mutagenic DNA repair ; RAD4 and REV2 gene cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The RAD4 gene of yeast required for the incision step of DNA excision repair and the REV2 (= RAD5) gene involved in mutagenic DNA repair could not be isolated from genomic libraries propagated in E. coli regardless of copy number of the shuttle vector in yeast. Transformants with plasmids conferring UV resistance to a rad4-4 or a rev2-1 mutant were only recovered if yeast was transformed directly without previous amplification of the gene bank in E. coli. DNA preparations from these yeast clones yielded no transformants in E. coli but retransformation of yeast was possible. This lead to the isolation of a defective derivative of the rad4 complementing plasmid. The modified plasmid was now capable of transforming E. coli but still interfered significantly with its growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420608
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard (1981)
    Springer
    Current genetics 4 (1981), S. 145-149 
    Details
    ISSN: 1432-0983
    Keywords: Yeast mutants ; Nitrogen mustard ; Thermoconditional DNA repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1–2 ts showed mainly ts-sensitivity to HN2, whereas mutant allele snm2-1 ts conferred ts-sensitivity to HN2, half mustard (HN1) and UV. In temperature-shift experiments it was determined that the functions of SNM1 and SNM2 are needed for recovery within 6 to 7 h. after mutagen exposure during incubation at 23 °C on YEPD when HN2 and UV are applied. After HN1 treatment the SNM2 coded function is required for recovery for about 14 hrs. This possibly indicates a handling of UV- and HN2-induced lesions different from that of HN1-induced lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365693
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  • 6
    Electronic Resource
    Electronic Resource
    Hyperthermia and paraquat-induced G1 arrest in the yeastSaccharomyces cerevisiae is independent of the RAD9 gene (1996)
    Springer
    Radiation and environmental biophysics 35 (1996), S. 55-57 
    Details
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Mutants of the RAD9 gene ofSaccharomyces cerevisiae are defective in cell cycle checkpoint arrest in G1 and G2 after treatment with DNA-damaging agents. It is demonstrated that the pronounced G1 arrest observed in yeast after hyperthermia treatment or exposure to paraquat-generated superoxide radicals does not depend on a functional RAD9 gene. For both types of treatments, the mechanism of cell cycle arrest must be considered different from the activation of the DNA damage checkpoint response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01211243
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  • 7
    Electronic Resource
    Electronic Resource
    Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 162-168 
    Details
    ISSN: 1617-4623
    Keywords: Key words DNA repair ; Regulation ; Gene fusion ; DRE element ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The interstrand cross-link repair gene SNM1 of Saccharomyces cerevisiae was examined for regulation in response to DNA-damaging agents. Induction of SNM1-lacZ fusions was detected in response to nitrogen mustard, cis-platinum (II) diamine dichloride, UV light, and 8-methoxypsoralen +UVA, but not after heat-shock treatment or incubation with 2-dimethylaminoethylchloride, methylmethane sulfonate or 4-nitroquinoline-N-oxide. The promoter of SNM1 contains a 15 bp motif, which shows homology to the DRE2 box of the RAD2 promoter. Similar motifs have been found in promoter regions of other damage-inducible DNA repair genes. Deletion of this motif results in loss of inducibility of SNM1. Also, a putative negative upstream regulation sequence was found to be responsible for repression of constitutive transcription of SNM1. Surprisingly, no inducibility of SNM1 was found after treatment with DNA-damaging agents in strains without an intact DUN1 gene, while regulation seems unchanged in sad1 mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174175
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard (1981)
    Springer
    Molecular genetics and genomics 181 (1981), S. 346-351 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants of Saccharomyces cerevisiae with enhanced sensitivity to the DNA cross-linking agent nitrogen mustard (HN2) have been isolated and partially characterized with respect to their phenotypic and genetic properties. The screening technique, based on HN2-sensitivity as sole criterion, yields approximately 1 sensitive isolate in 200 clones when applied to an intensively mutagenized population of a resistant parent strain. Mutants characterized so far are all due to recessive nuclear genes and represent at least seven complementation groups. They exhibit different degrees as well as different patterns of sensitivity towards monofunctional and bifunctional alkylating agents, and ultraviolet light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425609
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of mutagenic DNA repair in a thermoconditional repair mutant of Saccharomyces cerevisiae (1983)
    Springer
    Molecular genetics and genomics 190 (1983), S. 406-412 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using the thermoconditional yeast mutant rev2 ts that controls an apparently site-specific step of mutagenic DNA repair it was possible to measure the time course of REV2 dependent UV-induced reversion of the ochre allele his5-2 and recovery of survival for UV-treated stationary phase cells: due to the rev2 ts coded protein being active at 23° C, survival and mutation frequencies increased with duration of incubation under permissive conditions in growth medium before the temperature was shifted to 36° C (restrictive temperature). This increase was abolished in the presence of the protein synthesis inhibitor, cycloheximide. Furthermore, the REV2 dependent recovery of survival could be blocked or nearly blocked by cycloheximide added at any time during repair. Therefore, REV2 dependent repair can be characterized as a process requiring concomitant protein synthesis. These findings give further support to the concept that in yeast, mutagenesis involves UV inducible components of DNA repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331068
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of mutagenic DNA repair in a thermoconditional repair mutant of Saccharomyces cerevisiae (1983)
    Springer
    Molecular genetics and genomics 190 (1983), S. 413-416 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The time course of REV2 dependent recovery from prelethal UV damage and UV-induced locus-specific reversion of the his5-2 allele was determined in temperatureshift experiments by use of a thermoconditional allele of the rev2 gene (rad5-8, rev2 ts). In UV-irradiated, exponentially growing rev2 ts cells the REV2 dependent repair activity persists for up to 8 h at permissive temperature (23° C), while the REV2 dependent mutagenic process is mostly completed within 2 h. The REV2 dependent process in exponentially growing cells is highly impaired by inhibition of protein synthesis. However, a REV2 dependent repair activity independent of de novo synthesis is detectable, even in the presence of up to 200 μg/ml cycloheximide, a response not found in stationary phase cells. Thus, the REV2 dependent process seems to be partially constitutive in exponentially growing cells. Additionally, exponentially growing rev2 ts cells were considerably more UV-sensitive at restrictive temperature (36°C) than were stationary phase cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331069
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