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Specific apocrine secretion in the anterior lobe of the prostate gland of rabbits (1974)

Nicander, L. ; Plöen, L. ; Larsson, M.

Springer

Cell & tissue research 151 (1974), S. 69-77

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ISSN: 1432-0878

Keywords: Prostate (rabbit) ; Anterior lobe ; Apocrine secretion ; Ultrastructure ; Protein secretion

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The structure of glandular cells in the anterior lobe of the rabbit prostate was reinvestigated, especially after fixation by vascular perfusion with glutaraldehyde. The lumina of the gland contain abundant globules of an amorphous material surrounded by a membrane. A few dense bodies and scattered globules with internal vesicles also occur. These luminar contents obviously correspond to the particles observed in the semen of rabbits. Strong evidence was found for the formation of the globules by an apocrine secretory process. Material similar to the contents of the luminar globules accumulates in the cell apex together with vesicles, probably of Golgi origin, but without any other organelles. Protrusions from the cell surface contain similar material, but the vesicles generally disappear before the forming globule is finally pinched off from the cell surface. The characteristic feature of the secretory cell are the paucity of endoplasmic reticulum and the large amounts of ribosomes. This is interpreted as a specialization of the cell for the production of secretory protein that is not segregated within membranes until it is discharged from the cell by apocrine secretion.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00222035

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Nucleus of the boar spermatozoon: Structure and modifications in frozen, frozen-thawed, or sodium dodecyl sulfate-treated cells (1989)

Courtens, J. L. ; Paquignon, M. ; Blaise, F. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Molecular Reproduction and Development 1 (1989), S. 264-277

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ISSN: 1040-452X

Keywords: Nucleoproteins ; Element concentrations ; Electron microscopy ; Image analysis ; X-ray spectrophotometry ; Flow cytofluorometry ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: After cryosubstitution and Epon embedding, or after Nanoplast embedding and very thin sectioning, the chromatin of ejaculated or diluted boar spermatozoa appears to be formed of DNA fibers embedded in a quite homogeneous matrix. After sodium dodecyl sulfate (SDS) treatment, and to a lesser extent after freeze-thawing, the DNA fibers are present mostly between cords, probably proteinaceous in nature. The quantity of free sulfhydryl (SH) groups, as calculated from staining by DACM and flow fluorometry, is increased in thawed or SDS-treated cells. The quantity of NH2 groups, calculated from electron microscopy image analysis of alcoholic phosphotungstic acid-stained cells, is decreased in thawed nuclei. The DNA is more accessible to the fluorochrome ethidium bromide after freeze-thawing, and its sensitivity to HCI hydrolysis is modified, during the Feulgen-like staining procedure using acriflavine. The X-ray energy dispersive analysis of cryosections of nuclei indicates that the slight separation of DNA and nucleoproteins in freeze-thawed spermatozoa could result from a dramatic modification of the nuclear ionic environment during thawing.

Additional Material: 17 Ill.