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  • 1
    Electronic Resource
    Electronic Resource
    The Morphology of 16 and 17 Day Old Bovine Blastocysts from Virgin and Repeat Breeder Heifers (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 15 (1986), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The morphology of 16–17 days old embryos from virgin heifers (VH) and repeat breeder heifers (RBH) was compared using light and electron microscopy. In addition some embryos transferred from one heifer category to the other were studied. Embryos from VH were elongated blastocysts and the oval embryonic disc had three germ layers. The ectoderm was stratified and many mitoses were seen. The endoderm lining the blastocoelic cavity consisted of almost squamous cells conjoined by tight junctions. Between the ectoderm and the endoderm the mesoderm had developed and expanded laterally and the coelom had formed. The trophoblastic cells adjacent do the embryonic disc were cylindrical, whereas those more peripheral located were cuboidal. The trophoblastic cells were conjoined by tight junctions and they had numerous long microvilli on their peripheral surface. Except in the embryonic disc region, the endodermal cells had filopodial processes towards the trophoblast. The embryos from RBH varied in appearance. One was similar to those from VH whereas the others were, more or less retarded, without formation of mesoderm. The smaller one consisted ot trophoblastic cells only. The transferred embryos (representing surviving embryos: 2 out of 9 in VH-RBH and 5 of 6 in RBH-VH) had a morphology similar to that of VH blastocytes two though, appeared somewhat retarded. It is suggested that the retarded embryos lack the ability to complete embryonic development and that the uterine environment of RBH is not favourable to sustain normal embryonic development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1986.tb00719.x
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  • 2
    Electronic Resource
    Electronic Resource
    On the Ultrastructure of Seven Day Old Normal (Blastocysts) and Abnormal Bovine Embryos (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 10 (1981), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Twenty-four 7 day old bovine embryos were collected from spontaneously ovulating heifers. The embryos were classified into three groups; normal, morphologically deviating or degenerated. Electron microscopy was used to ascertain the ultrastructural correlates to the phase contrast microscope classification. This study sustains the conclusion that only those embryos classified as normal blastocysts are likely to undergo further development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1981.tb00519.x
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  • 3
    Electronic Resource
    Electronic Resource
    Fine Structure of Spermatogenesis and Sertoli Cells (Epitheliocytus sustentans) in the Bull (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 15 (1986), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The fine structure of spermatogenesis and Sertoli cells in bulls has been described. New observations and data from the literature have been compiled to give a comprehensive description of the complicated differentiation processes and cellular relations in the seminiferous epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1986.tb00529.x
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  • 4
    Electronic Resource
    Electronic Resource
    The mammalian tubuli recti: Ultrastructural study (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 192 (1978), S. 1-17 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastructure of the tubuli recti was studied in the testes of sexually mature bulls, boars, rams, goats, rabbits and rats fixed by vascular perfusion. The tubuli recti are lined with a simple epithelium that varies in height, from squamous to tall columnar according to the species and the region. The cells are characterized by extensive lateral and tortuous basal plasma membranes and a luminal border with microvilli. Tight junctions and desmosomes are found in the upper half of the lateral borders. The Golgi apparatus is sizable and associated with it are coated vesicles and may smooth vesicles concentrated towards the luminal border. A distal segment of the tubuli recti is found in bulls only and is characterized by a high epithelium which is thrown into folds giving the lumen a festooned appearance. It is suggested that the epithelial cells of the tubuli recti are involved in fluid exchange and in the removal of unwanted spermatozoa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091920102
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  • 5
    Electronic Resource
    Electronic Resource
    Specific apocrine secretion in the anterior lobe of the prostate gland of rabbits (1974)
    Springer
    Cell & tissue research 151 (1974), S. 69-77 
    Details
    ISSN: 1432-0878
    Keywords: Prostate (rabbit) ; Anterior lobe ; Apocrine secretion ; Ultrastructure ; Protein secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of glandular cells in the anterior lobe of the rabbit prostate was reinvestigated, especially after fixation by vascular perfusion with glutaraldehyde. The lumina of the gland contain abundant globules of an amorphous material surrounded by a membrane. A few dense bodies and scattered globules with internal vesicles also occur. These luminar contents obviously correspond to the particles observed in the semen of rabbits. Strong evidence was found for the formation of the globules by an apocrine secretory process. Material similar to the contents of the luminar globules accumulates in the cell apex together with vesicles, probably of Golgi origin, but without any other organelles. Protrusions from the cell surface contain similar material, but the vesicles generally disappear before the forming globule is finally pinched off from the cell surface. The characteristic feature of the secretory cell are the paucity of endoplasmic reticulum and the large amounts of ribosomes. This is interpreted as a specialization of the cell for the production of secretory protein that is not segregated within membranes until it is discharged from the cell by apocrine secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222035
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  • 6
    Electronic Resource
    Electronic Resource
    Comparison of nuclear hydration in boar spermatozoa before and after freeze-thawing: Contrast analysis of cells embedded in bromide-labeled medium (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 21 (1992), S. 249-254 
    Details
    ISSN: 1059-910X
    Keywords: Electron microscopy ; Water ; Image analysis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Epon labeled with bromide was used to embed ejaculated and freeze-thawed spermatozoa, with the hypothesis that it replaces most of cell water. Image analysis of relative contrasts between sperm nuclei and the surrounding medium revealed that when used in low concentrations, bromide is mostly absorbed to the nuclear structures. For higher concentrations, the chromatin is saturated, and the increase in contrast can be used to calculate relative differences in the hydration of nuclei. Boar sperm nuclei are more hydrated after freeze-thawing than before. © 1992 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070210308
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  • 7
    Electronic Resource
    Electronic Resource
    Nucleus of the boar spermatozoon: Structure and modifications in frozen, frozen-thawed, or sodium dodecyl sulfate-treated cells (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 1 (1989), S. 264-277 
    Details
    ISSN: 1040-452X
    Keywords: Nucleoproteins ; Element concentrations ; Electron microscopy ; Image analysis ; X-ray spectrophotometry ; Flow cytofluorometry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: After cryosubstitution and Epon embedding, or after Nanoplast embedding and very thin sectioning, the chromatin of ejaculated or diluted boar spermatozoa appears to be formed of DNA fibers embedded in a quite homogeneous matrix. After sodium dodecyl sulfate (SDS) treatment, and to a lesser extent after freeze-thawing, the DNA fibers are present mostly between cords, probably proteinaceous in nature. The quantity of free sulfhydryl (SH) groups, as calculated from staining by DACM and flow fluorometry, is increased in thawed or SDS-treated cells. The quantity of NH2 groups, calculated from electron microscopy image analysis of alcoholic phosphotungstic acid-stained cells, is decreased in thawed nuclei. The DNA is more accessible to the fluorochrome ethidium bromide after freeze-thawing, and its sensitivity to HCI hydrolysis is modified, during the Feulgen-like staining procedure using acriflavine. The X-ray energy dispersive analysis of cryosections of nuclei indicates that the slight separation of DNA and nucleoproteins in freeze-thawed spermatozoa could result from a dramatic modification of the nuclear ionic environment during thawing.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080010407
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