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  • 1
    Electronic Resource
    Electronic Resource
    Profilin gene expression and regulation in a temperature-sensitive breast cancer cell line: tsFT101 (1997)
    Springer
    Pflügers Archiv 434 (1997), S. 341-345 
    Details
    ISSN: 1432-2013
    Keywords: Key words Cell division ; Cytokinesis ; G-actin ; F-actin ; Multinuclear cells ; Profilin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The temperature-sensitive mutant cells (tsFT101) derived from a mouse mammary carcinoma cell line, FM3A, become multinucleated at a non-permissive temperature of 39°C. To further understand the molecular mechanism of such cytokinetic disturbance, we examined the expression of profilin, the main regulator of the transition of globular actin (G-actin) to filamentous actin (F-actin). RT-PCR analysis of mouse profilin cDNA from tsFT101 showed a point mutation (177 A → G) which was a wobble mutation causing no change in the encoded amino acid. The expression level of profilin mRNA was, however, diminished in cultured tsFT101 cells under non-permissive temperatures compared with wild-type FM3A cells in association with multinucleation. A stable transfection of profilin cDNA expression vector to tsFT101 cells prevented multinuclear cell formation when cultured at 39°C. In contrast, antisense profilin cDNA expression vector did not alter multinuclear cell formation. The primary cause of the cytokinetic disturbance of tsFT101 cells may be due to the diminished level of profilin gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050406
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    TGF-β1 mediates 70-kDa heat shock protein induction due to ultraviolet irradiation in human skin fibroblasts (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 239-244 
    Details
    ISSN: 1432-2013
    Keywords: Key words Anti TGF-β receptor antibody ; Heat shock protein 70 ; Human skin fibroblast cell ; Transforming growth factor-β ; Ultraviolet B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Ultraviolet B (UVB) alters the expression of heat shock protein 70 (HSP70) in cultured fibroblast cells derived from human skin. However, the nature of the signal transduction pathway remains to be determined. Transforming growth factor-β (TGF-β) has a large variety of biological functions, including cell growth control, modulation of inflammation and immunoregulation. In this study, we examined whether TGF-β is associated with the process of HSP70 expression induced by UVB irradiation. The constitutive expression of TGF-β1 mRNA and HSP70 expression in human skin fibroblast cells were detected using reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. The results indicate that: (1) UVB irradiation stimulates HSP70 expression in a dose- and time-dependent manner, (2) constitutive expression of TGF-β1 mRNA is detected after UVB irradiation, the level of which peaks at 4 h after 10 mJ cm–2 of UVB irradiation, (3) HSP70 expression is induced by TGF-β1 without UVB irradiation, and (4) HSP70 expression induction with UVB irradiation is inhibited by preincubation of the cells with the anti-TGF-β type II receptor antibody. Our results suggest that HSP70 expression induced by UVB involves the autocrine signalling of TGF-β production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050905
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Distribution of the Parathyroid Hormone-Related Peptide and Its Receptor in the Saccus Vasculosus and Choroid Plexus in the Red Stingray (Dasyatis akajei: Elasmobranch) (1998)
    Springer
    Cellular and molecular neurobiology 18 (1998), S. 361-368 
    Details
    ISSN: 1573-6830
    Keywords: saccus vasculosus ; nucleus of the saccus vasculosus ; choroid plexus ; parathyroid hormone-related peptide (PTHrP) ; PTHrP receptor ; red stingray ; immunohistochemistry ; immunoradiometric assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. The exact role of the parathyroid hormone-related peptide (PTHrP) is not fully understood. We used immunohistochemistry to localize the PTHrP and its receptor in the brain of the red stingray, particularly in the saccus vasculosus (SV) and choroid plexus. 2. Immunoreactive PTHrP and its receptor were detected in the epithelial cells of the SV and the choroid plexus. In addition, the neuronal perikarya in the nucleus of the SV located in the hypothalamus is positive for the PTHrP. 3. No PTHrP-containing neurons were detected in the choroid plexus. Extracts of SV and choroid plexus showed positive reactions against the PTHrP and its receptor antibody in Western blot analysis. 4. High levels of immunoreactive PTHrP were detected in the plasma equivalent to those present in human humoral malignant hypercalcemia. In contrast, the immunoreactive PTHrP concentration in the cerebrospinal fluid was below detectable levels. 5. Our results suggest that the regulation of the PTHrP in the SV differs from that in the choroid plexus in the red stingray.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022509300758
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    Paper (German National Licenses)
    Fulltext
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