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Complex between α-Chymotrypsin and poly(ethylene glycol) catalytically active in organic media (1999)

Murakami, Yoshihiko ; Hirata, Akira

Springer

Biotechnology techniques 13 (1999), S. 545-548

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ISSN: 1573-6784

Keywords: α-chymotrypsin ; enzyme modification ; nonaqueous enzymology ; organic media ; polymer/enzyme complex

Source: Springer Online Journal Archives 1860-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract α-Chymotrypsin and poly(ethylene glycol) were mixed in an aqueous phosphate buffer solution and lyophilized. The preparation was readily soluble in anhydrous isooctane. The polymer/enzyme complex, i.e., poly(ethylene glycol)/α-chymotrypsin, was catalytically active in anhydrous isooctane even when the molecular ratio of them was unity.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008987431361

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Pulsed column reactor as a novel tool for continuous enzymatic synthesis of peptide in an aqueous/organic biphasic medium (1999)

Murakami, Yoshihiko ; Hayashi, Shigemitsu ; Takehara, Akinori ; [et al.]

Springer

Biotechnology techniques 13 (1999), S. 165-168

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ISSN: 1573-6784

Keywords: pulsed column reactor ; enzymatic peptide synthesis ; aqueous/organic biphasic medium ; aspartame ; thermolysin

Source: Springer Online Journal Archives 1860-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract We propose a novel effective method for a continuous peptide synthesis in an aqueous/organic biphasic medium using a pulsed column reactor. N-Formyl-l-aspartyl-l-phenylalanine methyl ester was enzymatically synthesized continuously. With this extractive method using a pulsed column reactor, we can synthesize peptides with a stable performance even if a peptide (or a peptide-amino acid complex) is precipitated due to its high hydrophobicity.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008954129945

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