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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 36 (1971), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY —Small white potatoes were heated with microwave energy followed by boiling water to determine the penetration of heat, inactivation of peroxidase and firmness of the potatoes. Treatments consisted of heating potatoes with microwave energy for 0.5, 1, 1.5 and 2 min followed by boiling water for 0, 1, 2, 3, 4 and 5 min. Temperature measurements were made at depths of 0.75 and 1.5 cm using tubers with a mean radius of 1.95 cm. Peroxidase inactivation was measured along the radius of a slice removed from the equatorial region. Firmness was determined by the ALLO-Kramer shear press. Potatoes, when heated by microwave energy, became hot first at the core with a heat gradient developing toward the periphery. Boiling water produced a heat gradient from the periphery toward the core. Consequently, the tissue located about midway of the radius was subjected to the least amount of heat. The minimum time required to completely inactivate peroxidase and the firmness values of the potato tissue at the time of enzymatic inactivation were as follows: 1.5 min microwaves and 3 min boiling water, 119 lb shear force; and 2 min microwaves and 2 min boiling water, 124 lb shear force. Peroxidase was not completely destroyed when the potatoes were subjected to energy for 1 min or less followed by heating in boiling water up to 5 min.
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Mouse monoclonal antibodies (MAbs) were produced by using yellow grouper nervous necrosis virus (YGNNV) as an immunogen, isolated from infected yellow grouper, Epinephelus awoara (Temminck & Schlegel), and propagated in GB cells. In enzyme linked immunosorbent assay (ELISA), 43 hybridoma clones secreting MAbs strongly reacted with the purified virus. Ten of them showed a higher neutralization index (NI) value between 6.5 and 4.5 (log10 NI) than the other 33 MAbs against YGNNV infection in cell culture. All 10 MAbs belonged to the IgG isotype with a κ light chain and recognized the 42 kDa coat protein of YGNNV by Western blot analysis. Immunohistochemical results demonstrated that the viral signals co-located with pathological lesions observed in retina, brain and spinal cord. These results indicate that the MAbs are useful for confirmative diagnosis of YGNNV infection.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Mycobacterium spp. isolated from food and ornamental fish in Thailand (TB1, TB40, TB267 and TB268), and the type strains Mycobacterium marinum (NCIMB 1298), M. fortuitum (NCIMB 1294) and M. chelonae (NCIMB 1474) were cultured in Long’s medium, Eagle’s minimum essential medium, Sauton’s medium and modified Sauton’s medium. The latter enabled excellent growth and production of extracellular products (ECP) from TB 40, TB267, TB268 and M. marinum NCIMB 1298 in particular, whereas growth and production of ECP for all strains was limited in Long’s medium. SDS-PAGE protein profiles of ECPs from 14-day culture supernatants showed major bands at 65 and 〈14 kDa. After 2 days culture at the higher temperature of 37°C (heat shock), the production of ECP from all mycobacteria strains except M. marinum averaged approximately four- to 10-fold higher than from strains cultured for 14 days at 28°C. Enzyme testing for the type strains indicated only mucinase activity for M. marinum, while lipase and RNase activities were detected for M. chelonae and M. fortuitum. Protease and DNase activities could not be detected for any of the Mycobacterium spp. tested. The medium lethal dose (LD50) of ECP to rainbow trout and Nile tilapia was greater than 400 μg protein fish–1.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. A rickettsia-like organism was observed in diseased Nile tilapia, Oreochronuis niloticus (L.), from southern Taiwan. Most affected fish were pale and lethargic with haemorrhages and ulcers on the skin. The most significant gross pathological changes were varying degrees of ascites, and enlargement of the spleen, kidney and liver. Marked white nodules (as ring-shaped foci), varying in size, were found in these organs. A Gram-negative, rickettsia-like organism (RLO) frequently appeared as inclusions or within host cell intra-cytoplasmic vacuoles. Fibrin thrombi, perivascular necrosis, chronic inflammatory cells with hypertrophy and RLO-laden cells were characteristic of the disease. White nodules induced in experimental fish were similar to naturally infected cases and RLOs were reisolated in tissue culture using a CHSE-214 cell line. This provides evidence that the systemic granulomas in Nile tilapia were caused by RLOs.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 15 (1992), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 15 (1992), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The Formosa snakehead, Channa maculata (Lacepède), and the largemouth bass, Micropterus salmoides (Lacepède), were given an intraperitoneal (i.p.) and/or intramuscular (i.m.) inoculation of Nocardia asteroides isolated from cultured Formosa snakehead, Typical granulomatous lesions induced by the experimental fish were similar to the naturally infected cases. The Formosa snakehead showed 100% and 93% mortality at 14 and 35 days after i.m. inoculation with 8 mg and 0·8 mg of bacterial suspension, respectively. The largemouth bass was slightly resistant to N. asteroides but still had 95% mortality at 35 days after inoculation (i.m.) with 8 mg bacterial suspension. The bacteria were easily reisolated from spleen and kidney of the experimental fish. Many typical multifocal granulomata were found at the sites of injection and the internal organs such as liver, spleen, kidneys, pancreas, intestine, gills, eyes and brain. The result indicated that the N. asteroides isolated from the field ease was pathogenic to Formosa snakehead and largemouth bass.
    Type of Medium: Electronic Resource
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  • 17
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The production of macrophage activation factor (MAF) by rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes was examined after culturing in vitro with extracellular products (ECP) collected from Mycobacterium sp. Cultures of leucocytes were prepared from naive fish, or fish previously vaccinated with either the ECP or with formalin killed whole cell preparations (WC) of the bacterium. The cells were then incubated with the ECP in vitro and the ability of their supernatants to activate macrophages assessed. Macrophages from control fish were incubated with the supernatants, and their ability to reduce nitroblue tetrazolium (NBT) measured as an indicator of macrophage activation. Incubation of head kidney macrophages from naive fish directly with 1, 10 or 100 μg mL–1 of ECP for 48 h significantly enhanced macrophage activation compared with control macrophages. Vaccination of fish with either ECP or WC had no significant effect on the respiratory burst of control macrophages 4 weeks post-vaccination. By the eighth week, however, absorbance levels of respiratory burst reflecting both the primary (cells from vaccinated fish cultured in vitro with PBS) and the secondary (cells cultured in vitro with ECP) MAF responses of fish vaccinated with ECP and WC, had peaked and these were significantly different from the non-vaccinated controls. This activity had fallen to levels similar to control fish by week 12 for fish vaccinated with WC.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 23 (2000), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 19
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A nodavirus was isolated from diseased yellow grouper, Epinephelus awoara, larvae cultured in southern Taiwan. The histopathology and RT–PCR results confirmed that it was a fish nodavirus; its coat protein gene sequence was similar to that of red spotted grouper nervous necrosis virus (RGNNV) and it is named yellow grouper nervous necrosis virus (YGNNV). A new nodavirus-susceptible cell line, grouper brain (GB) was established and characterized from the brain tissue of yellow grouper. The GB cells multiplied well in Leibovitz’s L-15 medium supplemented with 10% foetal bovine serum at temperatures between 24 and 32 °C, and have been subcultured more than 80 times, becoming a continuous cell line. The GB cell line consists of fibroblast-like cells and some epithelioid cells. The cell line yielded titres of YGNNV up to 108.5 TCID50 mL–1. The GB cells effectively replicated the virus at 28 °C, which could be purified to homogeneity by caesium chloride gradient centrifugation. Electron microscopy studies showed that purified virus particles were 25–30 nm in diameter. The cytoplasm of infected cells was filled with aggregates of virus particles. These results indicate that the GB cell line is a significant tool for the study of fish nodaviruses.
    Type of Medium: Electronic Resource
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  • 20
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Cultured cobia, Rachycentron canadum, of 45–80 g exhibited anaemia and ascites, and a mottled red and grey, extremely enlarged kidney with cream-coloured patches or spherical nodules. Cumulative mortality was about 90% within 1 month. Extrasporogonic or sporogonic stages of a myxosporean appeared in the blood, glomerulus, renal tubules and renal interstitium. The renal tubules were the main target tissue of the parasite and were completely occluded by sporogonic pseudoplasmodia at various degrees of maturity. Many sporogonic stages were attached to the brush border of the epithelium of the renal tubules. Mature spores were seen in the lumen of the tubules. They were elongated or spherical with numerous refractile granules in the cytoplasm. The polar filament formed 3–5 coils. No bacteria or viruses were isolated from the diseased fish. Based on the results of microbiological, histopathological and electron microscopical examinations, the cobia disease was believed to be caused by a Sphaerospora-like myxosporean. This is the first report of a myxosporean in cobia in aquaculture.
    Type of Medium: Electronic Resource
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