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  • 1
    Electronic Resource
    Electronic Resource
    p27 expression and gastric carcinoma (1997)
    [s.l.] : Nature Publishing Group
    Nature medicine 3 (1997), S. 593-593 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] To the editor — A cyclin-dependent kinase inhibitor, p27rapl, regulates progression from Gl into S phase. We read with great interest that the decrease or absence of p27 protein expression is a powerful negative prognostic marker in patients with breast1 and colorectal carcinomas2 and is a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm0697-593
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of ornithine decarboxylase messenger ribonucleic acid expression in colorectal carcinoma (1997)
    Springer
    Diseases of the colon & rectum 40 (1997), S. 1095-1100 
    Details
    ISSN: 1530-0358
    Keywords: Ornithine decarboxylase ; c-myc ; Proto-oncogene ; Reverse transcriptase-polymerase chain reaction ; Colorectal carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract PURPOSE: Ornithine decarboxylase (ODC) is a rate-limiting enzyme for polyamine synthesis. An elevated protein level of ODC was observed in the tumors. There has been, however, little information reported so far on the expression of ODC messenger ribonucleic acid (mRNA) in clinical colorectal carcinomas.In vitro studies disclosed that the transcriptions of the ODC gene is regulated by the c-myc gene. METHODS: The expression of ODC and c-myc mRNA in biopsy specimens obtained from both tumor tissue and the corresponding normal tissue was examined by the reverse transcriptase polymerase chain reaction method in 40 cases of colorectal carcinoma. RESULTS: The expression of ODC mRNA was observed in both tumor tissue and normal tissue. The tumor to normal ratio of ODC mRNA was higher in cases with deeply invasive tumors than in cases with shallow tumors, and it was also higher in Dukes B or C cases than in Dukes A cases. There was a significant correlation between the tumor to normal ratio of c-myc mRNA and that of ODC mRNA in each case. CONCLUSIONS: These findings suggested that 1) the study of the expression of ODC mRNA may be useful for preoperatively predicting more advanced disease of colon carcinoma, and 2) there was a significant correlation between expression of ODC and c-myc mRNA in the clinical samples, which was similar to the findings of a previous in vitro study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02050936
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  • 3
    Electronic Resource
    Electronic Resource
    Enhanced chemosensitivity of cells from malignant effusions under condition of exposure to high temperature (1986)
    Springer
    Surgery today 16 (1986), S. 323-329 
    Details
    ISSN: 1436-2813
    Keywords: clonogenic assay ; hyperthermia ; anticancer agents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Utilizing a clonogenic assay, the effects of hyperthermia and selected chemotherapeutic drugs on growth of cells from malignant effusions were studied. Fourteen of 25 samples obtained from 25 patients with various carcinomas formed at least 30 colonies per plate. Exposure of the cells to heat at 42°C for 1 hr before the plating slightly inhibited the colony growth. The drugs, adriamycin (AM) and mitomycin C (MMC), were tested at 3 different concentrations. When the cells were treated with these two drugs for 1 hr at 42°C, the percent of surviving colonies was significantly decreased, as compared to findings at 37°C, in both groups, at 3 different concentrations. The combination of drugs and hyperthermia appeared to function synergistically in one-third of such cases. These results suggest that cells from malignant effusions in patients with various carcinomas were more sensitive to AM or MMC, under condition of a higher temperature (42°C).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02470554
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  • 4
    Electronic Resource
    Electronic Resource
    Cell-mediated cytotoxic activity of regional lymph node cells from patients with gastric carcinoma (1987)
    Springer
    Surgery today 17 (1987), S. 83-90 
    Details
    ISSN: 1436-2813
    Keywords: cell-mediated cytotoxicity ; regional lymph nodes ; gastric carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The cell-mediated cytotoxic activities of cells from the perigastric lymph nodes (LNC) were assayed in patients with gastric carcinoma. These activities were compared with those of the peripheral blood mononuclear cells (PBM), and also with the LNC of patients with benign lesions. The capacity of LNC to be converted to cytotoxic cells in mixed cell culture was significantly impaired in the cancer patients as compared to that of either the PBM from the same patients, or the LNC from patients with benign lesions. The natural killer cell (NK) activity of LNC was significantly lower than that of the PBM in both groups of patients. The cytotoxic activity induced by phytohemagglutinin activation (PAK) in the LNC from patients with carcinoma, as well as from those with benign lesions was also significantly decreased, when compared to that of the PBM, although the ability of LNC to produce interleukin 2 (IL 2) was significantly increased. The ability of these cells to generate cytotoxicity after activation with IL 2 (LAK) was therefore examined, and a decreased capacity in LNC was observed. These results indicated that the ability of T cells in LNC to develop into cytotoxic cells in patients with gastric carcinoma was impaired, and that the nonspecific cytotoxicity, including NK or PAK, as well as LAK activity, was essentially low in the perigastric lymph nodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02470646
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  • 5
    Electronic Resource
    Electronic Resource
    Depression of the generation of cell-mediated cytotoxicity after surgery (1983)
    Springer
    Surgery today 13 (1983), S. 191-195 
    Details
    ISSN: 1436-2813
    Keywords: depression of cell-mediated cytotoxicity ; operative stress ; malignant lesions ; benign lesions ; Raji cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of surgery on generation of cell-mediated cytotoxicity in mixed cell cultures were studied in patients with various carcinomas, and the results were compared to those in patients with benign lesion. Peripheral mononuclear cells were cultured with the B-lymphoblastoid cell Raji in a mixed culture and the induced cytotoxicity was measured by51Cr release assay. In 15 patients with various carcinomas, the capacity of peripheral mononuclear cells to generate cytotoxic cells was significantly depressed 1, 3 and 6 days after surgery, as compared to findings before surgery. Preoperative levels were reverted to by the 8th postoperative day. In 8 patients with benign lesion, significant decreases in cytotoxic cell activity were observed 3 and 6 days after operation and, on the 8th postoperative day, there was a significant increase in the generated cytotoxicity, as compared to the preoperative activity. Thus, postoperative depression of the generation of cell-mediated cytotoxicity may affect the host-tumor relationship in patients with carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02469475
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  • 6
    Electronic Resource
    Electronic Resource
    Combination chemoimmunotherapy for advanced gastric carcinoma (1984)
    Springer
    Surgery today 14 (1984), S. 185-190 
    Details
    ISSN: 1436-2813
    Keywords: gastric carcinoma ; chemo-immunotherapy ; survival rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Eighty-nine patients with advanced gastric carcinoma were treated with a combination chemo-immunotherapy regimen that consisted of active immunotherapy with Vibrio cholerae neuraminidase (VCN) treated autologous tumor cells admixed with BCG and drugs including cyclophosphamide, mitomycin C (MMC) and 5-fluorouracil, followed by long term tegafur (FT) and immunomodulators. This treatment significantly improved survival rate of patients in Stages III, IV and unresectable or recurrent carcinoma, compared to that of historical controls. As compared to controls treated with MMC followed by long term FT and immunodulators concurrently, survival rate of those in Stage III tended to improve (p〈0.1) and survival rate at 4.5 years in Stage III was significantly higher (p〈0.01), although it was not improved in Stage IV. In patients with unresectable or recurrent tumor, survival time was not significantly lengthened with this therapy when compared with that in patients given BCG alone in the same treatment schedule (CCI-BCG group). However, none of 19 patients in CCI-BCG group survived more than 15 months, although 4 of 28 patients receiving this therapy survived. These results suggest that this combination chemo-immunotherapy is effective for a selected group of patients with advanced gastric carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02469566
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  • 7
    Electronic Resource
    Electronic Resource
    Neuroleptic malignant syndrome occurring after an emergency operation for traumatic duodenal perforation: Report of a case (1994)
    Springer
    Surgery today 24 (1994), S. 276-279 
    Details
    ISSN: 1436-2813
    Keywords: neuroleptic malignant syndrome ; postoperative complication ; differential diagnosis ; traumatic duodenal perforation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Neuroleptic malignant syndrome (NMS) is a potentially fatal complication which may develop in psychiatric patients taking neuroleptic drugs. We report herein the successful treatment of a 33-year-old schizophrenic man, prescribed neuroleptic drugs, who underwent an emergency operation for traumatic duodenal perforation with a retroperitoneal infection. Five days after the operation, he began to demonstrate clinical features consistent with NMS such as high fever, abnormalities in vital signs, leukocytosis, and an elevated serum level of creatine phosphokinase; however, these findings were first presumed to be secondary to either the preexisting tissue injuries or to postoperative complications. A definite diagnosis of NMS was thus delayed until muscle rigidity and autonomic instability became evident. After a tentative diagnosis of NMS had been made, sodium dantrolene, a drug used specifically for the treatment of NMS, was administered and the patient's condition remarkably improved. Since NMS can be induced by either interrupting the course of neuroleptic drugs or by the additional administration of sedative drugs, and since its mortality rate is high if prompt and appropriate treatment is not carried out, surgeons should bear in mind the possibility of NMS developing postoperatively in psychiatric patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02032902
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  • 8
    Electronic Resource
    Electronic Resource
    A simplified method for determination of tritiated thymidine incorporation into cells from tissue in soft agar culture (1986)
    Springer
    Surgery today 16 (1986), S. 235-238 
    Details
    ISSN: 1436-2813
    Keywords: tritiated thymidine incorporation ; soft agar culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We designed a simple method forin vitro chemosensitivity testing of tritiated thymidine incorporation by cells from tumor tissue plated in soft agar. Cells from tumor tissue were cultured in a plastic dish, of which a Millipore membrane had been sealed to the underside, containing two layers of agar. After labeling the cells with tritiated thymidine, the dishes were placed in a boiling water bath to solubilize the agar. The labeled cells could be readily collected on the membrane by evacuating the solubilized medium through membrane filters sealed underside of the dish. Using this system, 11 (55 per cent) of 20 tumor specimens from 20 patients with various carcinomas showed an incorporation of over 200 dpm above background and 80 per cent or greater inhibition of thymidine uptake by sodium azide treatment. This method offered several advantages over the thymidine incorporation assay, including technical simplicity and a shorter time course.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02471099
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  • 9
    Electronic Resource
    Electronic Resource
    Immunodepression after surgery: Impaired production of interleukin 2 (1984)
    Springer
    Surgery today 14 (1984), S. 384-386 
    Details
    ISSN: 1436-2813
    Keywords: interleukin 2 ; surgical stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The capacity of peripheral blood mononuclear cells to produce interleukin 2 (IL 2) was studied serially before and after various surgical procedures in 34 patients. In the group of 6 patients who had minor surgery and were categorized into class 1, IL 2 activity did not differ significantly from the preoperative value, throughout the postoperative course. In the group of 14 class 2 patients who underwent major surgery, however, significant decreases in IL 2 activity were observed 1, 3 and 6 days after operation as compared to findings before surgery. Preoperative levels were reverted to by the 8th postoperative day. The remaining 14 patients underwent major and extensive surgical procedures, and were put into class 3. Here too, were also significant decreases in the activity 1, 3 and 6 days after operation. The activity remained significantly depressed 8 days after surgery, in this group of patients. These results show that depression in the production of IL 2 in postoperative surgical patients correlates relatively well with the extent of the surgical procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02469545
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  • 10
    Electronic Resource
    Electronic Resource
    Apoptosis in antibody-dependent monocyte-mediated cytotoxicity with monoclonal antibody 17-1A against human colorectal carcinoma cells: enhancement with interferon γ (1996)
    Springer
    Cancer immunology immunotherapy 43 (1996), S. 220-225 
    Details
    ISSN: 1432-0851
    Keywords: Key words Apoptosis ; Antibody-dependent cell-mediated cytotoxicity ; Monocyte ; 17-1A ; Interferon γ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Antibody-dependent cell-mediated cytotoxicity (ADCC) has been considered to be one of the main effector mechanisms by which unconjugated monoclonal antibody (mAb) 17-1A can exert an antitumor effect in vivo. Since the apoptotic pathway as well as the necrotic pathway have been shown to be utilized in various cytotoxic effector mechanisms, we investigated the role of apoptosis in ADCC mediated by monocytes (ADMC) using mAb 17-1A as an antibody and the human colorectal carcinoma cell line, COLO205, as target cells in vitro. The implications of the apoptosis during ADMC was demonstrated by means of both a DNA fragmentation assay and a TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay. Furthermore, interferon γ (IFNγ) was also found to enhance the induction of apoptosis significantly. The addition of superoxide dismutase did not reduce the level of the apoptosis, although superoxide anion (O2 –) was observed to be produced. However, the release of tumor necrosis factor α (TNFα) was significantly enhanced during ADMC, while, in addition, apoptosis was significantly inhibited by the addition of anti-TNFα antibody. These findings indicated that apoptosis might be implicated in ADMC with mAb 17-1A, which was augmented by IFNγ, while, in addition, TNFα may also be one of the major mediators of apoptosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050325
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