ISSN:
1573-4994
Keywords:
Multiplex dyes
;
coumarin
;
rhodamine
;
time resolved fluorescence spectroscopy
;
immunoassay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Physics
Notes:
Abstract To detect several antigens simultaneously, antibodies directed against different antigens were immobilized on a quartz surface. The antigens were tagged with multiplex, dyes, which show different fluorescence lifetimes but similar excitation and emission spectra. The antigens were detected by recognizing the characteristic fluorescence lifetime. Furthermore, the effect of labeling of the dye on the antigen molecules was examined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01876665
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