ISSN:
1573-4994
Schlagwort(e):
Multiplex dyes
;
coumarin
;
rhodamine
;
time resolved fluorescence spectroscopy
;
immunoassay
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Physik
Notizen:
Abstract To detect several antigens simultaneously, antibodies directed against different antigens were immobilized on a quartz surface. The antigens were tagged with multiplex, dyes, which show different fluorescence lifetimes but similar excitation and emission spectra. The antigens were detected by recognizing the characteristic fluorescence lifetime. Furthermore, the effect of labeling of the dye on the antigen molecules was examined.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01876665
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