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  • 1
    Electronic Resource
    Electronic Resource
    Peripheral blood mononuclear cells isolated from patients with diabetic nephropathy show increased activation of the oxidative-stress sensitive transcription factor NF-kB (1999)
    Springer
    Diabetologia 42 (1999), S. 222-232 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Oxidative stress ; nephropathy ; transcription factor NF-kB ; thrombomodulin ; thioctic acid.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Increased oxidative stress and subsequent activation of the transcription factor NF-kB has been linked to the development of late diabetic complications. To determine whether oxidative stress dependent NF-kB activation is evident in patients with diabetic nephropathy we used an Electrophoretic Mobility Shift Assay based semiquantitative detection system which enabled us to determine NF-kB activation in ex vivo isolated peripheral blood mononuclear cells. We examined 33 patients with diabetes mellitus (Type I and Type II). Patients with diabetic nephropathy showed higher NF-kB binding activity in Electrophoretic Mobility Shift Assays and stronger immunohistological staining for activated NF-kBp65 than patients without renal complications. NF-kB binding activity correlated with the degree of albuminuria (r = 0.316) and with thrombomodulin plasma concentrations (r = 0.33), indicative for albuminuria associated endothelial dysfunction. In a 3 day intervention study in which 600 mg of the antioxidant thioctic acid (α-lipoic acid) per day were given to nine patients with diabetic nephropathy oxidative stress in plasma samples was decreased by 48 % and NF-kB binding activity in ex vivo isolated peripheral blood mononuclear cells by 38 %.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051142
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs (1996)
    Springer
    Histochemistry and cell biology 105 (1996), S. 221-230 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The distribution of CD44s and CD44v molecules in normal and injured lung tissue of rats and minipigs was studied by examining the immunohistochemical binding of monoclonal antibodies against CD44 isoforms. We showed that the expression of CD44v and CD44s varies greatly among different pulmonary fibrosis samples and that some tissues express either enhanced expression of CD44s, particularly in the interstitium and on alveolar macrophages, or very low levels of CD44v in the alveolar epithelium. Normal type II pneumocytes expressed the CD44s and CD44v molecules at the basolateral aspect of the cell. Such localisation favours a role for CD44 in epithelial cell-fibroblast interaction during lung development and repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01462295
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Loss of caveolin expression in type I pneumocytes as an indicator of subcellular alterations during lung fibrogenesis (1997)
    Springer
    Histochemistry and cell biology 109 (1997), S. 41-48 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Caveolin is a major structural protein of caveolae, also known as plasmalemmal vesicles, which are particularly abundant in type I pneumocytes and capillary endothelial cells of lung parenchyma. Here we demonstrate that caveolin expression in the alveolar epithelium of rats and mini pigs is strikingly downregulated after irradiation-induced lung injury. Indirect immunoperoxidase staining with polyclonal anti-caveolin antibodies, confirmed by double fluorescence studies with type I cell-specific monoclonal anti-cytokeratin antibodies or lectins, revealed a dramatic loss of caveolin immunoreactivity in type I pneumocytes. In contrast, caveolin expression increased in endothelial cells. Immunoblotting of lung homogenates from normal and irradiated rats using specific anti-caveolin antibodies confirmed the presence of caveolin in normal tissue and its marked decrease of expression in fibrotic tissue. The loss of caveolin as an important structural protein of caveolae in alveolar epithelial cells may be an early indicator of serious type I cell injury during fibrogenesis. The increase of caveolin immunoreactivity in endothelia of blood vessels may indicate that different types of caveolae and/or different regulatory mechanisms of caveolin expression exist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050200
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    Paper (German National Licenses)
    Fulltext
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