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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 692 (1982), S. 69-80 
    ISSN: 0005-2736
    Keywords: (Na^+ + K^+)-ATPase ; (Porcine brain) ; Liposome ; Membrane reconstitution ; Na^+-Ca^2^+ antiporter
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0014-5793
    Keywords: Adhesion ; Bromelain F9 ; CD44 ; Human umbilical vein edothelial cell ; Peripheral blood lymphocyte
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Crystal Growth 88 (1988), S. 435-441 
    ISSN: 0022-0248
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Graefe's archive for clinical and experimental ophthalmology 178 (1969), S. 367-380 
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit Hilfe von Silberimprägnationsmethoden, Vital- und Übersichtsfärbungen sowie der Dopa-Reaktion wurden Vorkommen, Merkmale und Verteilung sog. „polygonaler Zellen“ im Hornhautepithel untersucht. Als Material dienten Hornhäute adulter Rinder, Kaninchen, Ratten und Mäuse sowie embryonale ] Hornhäute von Homo, Huhn, Kaninchen und Ratte. Die Arbeit führte zu folgenden Ergebnissen: 1. Das ausdifferenzierte Hornhautepithel der untersuchten Species enthält neben den Epithelzellen im engeren Sinne Zellen, die durch Silberimprägnation selektiv geschwärzt werden. Sie entsprechen den „polygonalen Zellen“ japanischer Autoren. Zur Darstellung eignen sich neben den bisher benutzten Verfahren auch Silberimprägnationsmethoden nach Abraham, Jabonero und del Rio Hortega. 2. Die „polygonalen Zellen“ sind vielgestaltig, mit kurzen Fortsätzen versehen und werden vorzugsweise innerhalb der basalen Schichten des Hornhautepithels angetroffen. Sie liegen einzeln oder zu Strängen vereinigt zwischen Gruppen von Epithelzellen im engeren Sinne. Ihre Anordnung kann mit einem Raumgitter verglichen werden, dessen Lücken durch nicht argyrophile Epithelzellen ausgefüllt werden. 3. Die „polygonalen Zellen“ des Hornhautepithels von Rind, Kaninchen, Ratte und Maus weisen in Form und Anordnung keine wesentlichen Unterschiede auf. 4. Die Basalschicht des Epithels der Rinderhornhaut besteht zu 5,6–9,6% aus „polygonalen Zellen“, von denen auf 1 mm2 545–978 entfallen. Sie sind am zahlreichsten in der Randzone der Cornea. Die Frequenzen der „polygonalen Zellen“ im peripheren, intermediären und zentralen Hornhautbereich verhalten sich wie 5∶3∶3. 5. Die „polygonalen Zellen“ lassen sich durch subepitheliale Injektion von Brillantkresylblau in die lebende Hornhaut vorübergehend selektiv anfärben. Das Resultat der Färbung entspricht in wesentlichen Punkten den Ergebnissen von Silberimprägnationen. 6. Alle Bemühungen, im Epithel fetaler bzw. embryonaler Hornhäute von Homo, Huhn, Kaninchen und Ratte Dopa-positive „polygonale Zellen“ nachzuweisen, schlugen fehl. Aus den Resultaten und den Angaben der Literatur werden folgende Schlüsse abgeleitet: Es besteht kein Zweifel, daß die von japanischen Autoren als „polygonale Zellen“ oder „weiße Melanocyten“ beschriebenen Zellen regulärer Bestandteil des Hornhautepithels von Vertebraten sind. Sehr wahrscheinlich handelt es sich um Zellen epithelialen Typs bestimmter Funktion oder bestimmten Funktionszustandes. Trotz der Vielfalt verfügbarer Informationen bedarf das klassische Problem der Zelldualität im Hornhautepithel weiterer Untersuchungen.
    Notes: Summary The occurence, characteristics and distribution of the so-called “polygonal cells” in the corneal epithelium were studied by means of silver impregnation methods, vital staining, common staining methods, and Dopa-reaction. Corneas of adult cows, rabbits, rats, and mice as well as embryonic corneas of man, fowl, rabbit, and rat were used. The results are as follows: 1. Besides the epithelial cells in the strict sense the corneal epithelium of the animals studied contains cells which are selectively blackened by silver impregnation. These cells correspond to the “polygonal cells” of Japanese authors. Besides the methods hitherto used silver impregnations after Abraham, Jabonero and del Rio Hortega are suitable as well for identification. 2. The “polygonal cells” are polymorphous, possess short processes and are chiefly found in the basal layers of the corneal epithelium. They are situated solitarily or arranged to cords between groups of epithelial cells in the strict sense. Their arrangement may be compared with a threedimensional lattice the gaps of which are filled by not argyrophil epithelial cells. 3. Differences of shape or arrangement between the “polygonal cells” of the corneal epithelium of cows, rabbits, rats and mice are apparently not fundamental. 4. The percentage of “polygonal cells” in the basal layer of the corneal epithelium in cows is 5.6–9.6. 546–978 “polygonal cells” were found within 1 mm2. They are most numerous near the limbus corneae. The proportion of frequencies of “polygonal cells” within the peripheral, intermediate and central zones of the cornea is about 5∶3∶3. 5. The “polygonal cells” are transiently stainable by subepithelial injection of brillant cresyl blue into the living cornea. In the main the staining result corresponds to the effects of silver impregnation. 6. All efforts to find Dopa-positive “polygonal cells” in fetal or embryonic corneas of man, fowl, rabbit, and rat were in vain. The results and the informations found in the literature lead to the following conclusions: There is no doubt, that the cells described as “polygonal cells” or “white melanocytes” by Japanese authors are a regular constituent of the corneal epithelium in vertebrates. They are probably cells of the epithelial type of a certain function or in a special functional resp. developmental state. In spite of the abundance of informations available the classical problem of cell duality in the corneal epithelium needs further investigation.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 126 (1995), S. 711-723 
    ISSN: 1434-4475
    Keywords: Malononitrile ; Benzoles ; Pyridines ; Thiophenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Pyridinium- und Sulfoniumsalze2a–e, die aus 3-Amino-2-(α-halo-acetyl)-crotonsäurenitril und 3-Amino-2-(α-halo-acetyl)-zimtsäurenitril hergestellt werden können, reagieren mit Malonsäuredinitril in Gegenwart einer Base zu 3-Amino-2-pyridinio-phenolaten3a,b und 3-Amino-2-sulfonio-phenolaten3c–e. In Analogie wurden 3-Amino-2-(diethyloxyphosphoryl)-phenol5a und 3-Amino-2-(p-tolyl-sulfonyl)-phenol5b erhalten. 2,3-Diamino-phenole6a,b werden aus den Pyridiniumsalzen3a,b gebildet. Das Verhalten der Pyridiniumsalze2a,b gegenüber Heterokumulenen ist untersucht worden. Cyanamid führt zum (2-Amino-4-hydroxy-pyrid-3-yl)-pyridiniumsalz8c. Phenylisothiocyanat liefert die 3-Pyridinio-2-thioxo-pyridin-4-olate9a,b. In Abhängigkeit vom Substituenten in der Position 6 entsteht bei der Reaktion mit Schwefelkohlenstoff ein 3-Pyridinio-2-thioxo-pyridin-4-olat10 oder ein 3-Pyridinio-2-thioxo-thiopyran-4-olat11. Phenylisocyanat reagiert zum Pyrimidin-2,4-dion12 unter Verlust von N-Methyl-pyridiniumchlorid. S-Methylierung von9a und Spaltung des Pyridiniumringes ergibt das 3-Amino-2-methylthio-pyrid-4-on14. Die Verbindungen wurden1H- und13C-NMR-spektroskopisch untersucht.
    Notes: Summary Pyridinium and sulfonium salts2a–e which can be prepared from 3-amino-2-(α-haloacetyl)-crotonitriles and 3-amino-2-(α-halo-acetyl)-3-phenyl-acrylonitriles react with malononitrile in the presence of a base to 3-amino-2-pyridinio-phenolates3a,b and 3-amino-2-sulfonio-phenolates3c–e. In an analogous way, 3-amino-2-(diethyloxyphosphoryl)-phenol5a and 3-amino-2-(p-tolyl-sulfonyl)-phenol5b have been prepared. 2,3-Diamino-phenoles6a,b are formed from the pyridinium salts3a,b. The behaviour of the pyridinium salts2a,b towards heterocumulenes has been investigated. Cyanamide leads to the (2-amino-4-hydroxy-pyrid-3-yl)-pyridinium salt8c. Phenylisothiocyanate gives the 3-pyridinio-2-thioxo-pyridin-4-olates9a,b. Carbon disulfide gives rise to 3-pyridinio-2-thioxo-pyridin-4-olate10 or 3-pyridinio-2-thioxo-thiopyran-4-olate11, depending on the substituent at the 6-position. Phenylisocyanate reacts to the pyrimidin-2,4-dione12 with loss of N-methyl-pyridinium chloride. S-methylation of9a and cleavage of the pyridine moiety yields the 3-amino-2-methylthio-pyrid-4-one14. The structures were investigated by1H and13C NMR spectroscopy.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1335
    Keywords: Colorectal cancer ; LAK cell activity ; Prothymosin α1 ; Cytokines ; Adhesion molecules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of prothymosin α1 (Pro α1) in combination with interleukin-2 (IL-2) on peripheral blood lymphocytes from 50 colorectal tumor patients at different stages were studied with respect to immunocytotoxicity, adhesion to cultured SW620 colon carcinoma cells, secretion of cytokines and expression of adhesion and surface marker molecules. On average, the patients showed lower natural killer (NK) cell activity than healthy donors, which was associated with a lower adhesion capacity to the tumor target cells. The NK cell activity of the patients was inversely related to the tumor stage. The generation of lymphokine(IL-2)-activated killer (LAK) cell activity was found to be comparable on lymphocytes from healthy individuals and patients and was not correlated to tumor stage. Pro α1 stimulated patients' LAK cell activity only, primarily at the early stage (Dukes A/B). The Pro α1 effect was associated with an increased adhesion of lymphocytes to tumor target cells and an increased secretion of the deficient IL-2-induced IFNγ secretion. No significant effects on the low level of TNFα secretion was noted. By flow cytometry, Pro α1 in combination with IL-2 augmented the expression of the NK cell markers CD56, CD16/56, the subset CD3/16/56 and CD25 on lymphocytes of the patients. In contrast, Pro α1 was equally effective by increasing the expression of CD18 and CD11a, on lymphocytes from the patients and from normal controls. In conclusion, Pro α1, in combination with IL-2, can partially normalize lymphocyte deficiencies of colon cancer patients in vitro. This potential might provide an experimental basis for applying Pro α1 or related thymic peptides in selected immunotherapies against colorectal tumors.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 70 (1999), S. 847-857 
    ISSN: 1433-0385
    Keywords: Key words: Biomaterials ; Biocompatibility ; Process engineering ; Reciprocal technology transfer. ; Schlüsselwörter: Biomaterialien ; Biokompatibilität ; Prozesstechnologien ; wechselseitiger Technologietransfer.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Biokompatible Werkstoffe und zugehörige Prozesstechnologien, mit denen eine optimale Strukur- und Oberflächenkompatibilität von Implantaten erreicht werden soll, werden vorgestellt. „Vital-avital composites“ für das „tissue engineering“, Zellkulturmodelle, poröse Keramiken und abbaubare Polymere werden beispielhaft dargestellt. Ein Hauptaspekt liegt auf der Konvertierung von Resultaten der Grundlagenforschung in klinische Anwendungen und auf dem Austausch von Technologien aus dem nichtmedizinischen in den medizinischen Bereich und umgekehrt.
    Notes: Summary. Biomaterials and related process engineering in order to obtain optimal surface and structural biocompatibility of implants and devices are presented. Vital-avital composites for tissue engineering, cell culture models, porous ceramics and degradable polymers are introduced as examples. Emphasis is laid on the conversion of basic research results into clinical applications and on the exchange of technologies from the non-medical into the medical field and vice versa.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 44 (1988), S. 249-251 
    ISSN: 1420-9071
    Keywords: EGF ; anti-EGF serum ; newborn mice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Administration of anti-EGF serum to newborn mice led to delay of eyelid opening and incisor tooth eruption, acceleration of hair growth and delay of weight gain. These results indicate that in the first week after birth EGF still has a physiological function, which can be abrogated by anti-EGF serum.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Intensive care medicine 22 (1996), S. 460-463 
    ISSN: 1432-1238
    Keywords: Key words Acute adrenocortical insufficiency ; Cardiomyopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  An 11-year-old boy in acute adrenal crisis was subsequently diagnosed with Addison’s disease and presumably had an unrecognized preceding chronic adrenal insufficiency. His adrenal insufficiency and hypovolemic shock were promptly diagnosed and treated in the emergency room. However, 24 h later, signs of congestive heart failure developed and an echocardiogram revealed cardiomyopathy. He responded to i.v. dopamine and his cardiomyopathy resolved over some days. Acute adrenal insufficiency and actual ventricular dysfunction had not been described in humans until last year; this is the second reported case of cardiomyopathy in a patient with acute adrenal insufficiency.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Intensive care medicine 22 (1996), S. 460-463 
    ISSN: 1432-1238
    Keywords: Acute adrenocortical insufficiency ; Cardiomyopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An 11-year-old boy in acute adrenal crisis was subsequently diagnosed with Addison's disease and presumably had an unrecognized preceding chronic adrenal insufficiency. His adrenal insufficiency and hypovolemic shock were promptly diagnosed and treated in the emergency room. However, 24 h later, signs of congestive heart failure developed and an echocardiogram revealed cardiomyopathy. He responded to i.v. dopamine and his cardiomyopathy resolved over some days. Acute adrenal insufficiency and actual ventricular dysfunction had not been described in humans until last year; this is the second reported case of cardiomyopathy in a patient with acute adrenal insufficiency.
    Type of Medium: Electronic Resource
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