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  • 1
    Electronic Resource
    Electronic Resource
    Mouse/human chimeric IgGl and IgG4 antibodies directed to the house dust mite allergen Der p 2: use in quantification of allergen specific IgG (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 27 (1997), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background and Objective Chimeric mouse/human monoclonal IgGl and IgG4 antibodies were developed against the house dust mite allergen Der p 2. These chimeric IgG antibodies, hIgG1-Dp2 A and hIgG4-Dp2 A, have the same binding characteristics as the previously reported chimeric hIgE-Dp2 A and are composed of the heavy chain variable domains and light chains ot the original murine monoclonal antibody 2B12., whereas the heavy chain constant domains have been replaced by the human IgGl or IgG4 heavy chain. The expression level of hIgG1-Dp2 A and hIgG4-Dp2 A was 1 and 3.5 μg/mL, respectively.Methods and Results Since all IgG in these culture supernatants is allergen-specific. they are useful reference reagents and enable the calculation of the amount of allergen specific IgG l and IgG4 antibodies in absolute IgG amounts. The results obtained with two panels of sera from patients in immunotherapeutic treatment were evaluated and compared in Der p 2 IgE, IgGl and IgG4 RAST and with reversed lgG4 RAST using labelled purified Der p 2. Close agreement between the results for the two IgG4 assays was found.Conclusion With these chimeric reference reagents the quantities of isotype specific antiallergen antibodies can be calculated and compared.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1997.tb01262.x
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  • 2
    Electronic Resource
    Electronic Resource
    Treatment of non anti-GBM-antibody mediated, rapidly progressive glomerulonephritis by plasmapheresis and immunosuppression (1986)
    Springer
    Journal of molecular medicine 64 (1986), S. 231-238 
    Details
    ISSN: 1432-1440
    Keywords: Rapidly progressive glomerulonephritis ; Prognosis ; Immunosuppressive drugs ; Plasma exchange ; Interstitial fibrosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A retrospective study was conducted to evaluate the efficacy of plasmapheresis in combination with different immunosuppressive drugs (“pulse” therapy, azathioprine or cyclophosphamide together with steroids) in nine patients presenting with rapidly progressive glomerulonephritis (RPGN) not mediated by antibody to glomerular basement membrane. Six of these patients had to be initially dialysed. All patients underwent renal biopsy, which revealed that seven patients had a minimum of 80% crescents and five had interstitial fibrosis. Recovery of renal function was observed in seven patients (78%). All patients without interstitial fibrosis were recompensated for at least 14 months after the acute onset of RPGN. Those who presented with interstitial fibrosis declined to endstage renal failure after 13 months requiring chronic hemodialysis treatment or cadaveric kidney transplantation. On the basis of these findings interstitial fibrosis seems to be a limiting factor for the prognosis of non-anti-GBM-antibody mediated RPGN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01711654
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  • 3
    Electronic Resource
    Electronic Resource
    The progression of renal diseases: On the pathogenesis of renal interstitial fibrosis (1991)
    Springer
    Journal of molecular medicine 69 (1991), S. 576-586 
    Details
    ISSN: 1432-1440
    Keywords: Renal interstitial fibrosis ; Fibroblast cell system ; Collagen synthesis ; Local immune responses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Renal interstitial fibrosis (RIF) frequently occurs in inflammatory and non-inflammatory kidney diseases and is associated with a decline in renal excretory function. Fibroblasts which occupy the renal interstitium are involved mainly in the formation of RIF not only by the production of extracellular matrix, but also by regulatory processes. They respond to a variety of cytokines released by different cell types. To investigate mechanisms leading to RIF, immunohistochemical analysis and cell cultures of renal biopsies in various renal diseases have been performed. T lymphocytes are the major cells infiltrating the renal interstitium, and their number correlates with the impairment of renal function. In most forms of glomerulonephritis accompanied by interstitial inflammation, an abnormal expression of HLA-DQ/-DP molecules, frequently associated with an aberrant expression of the intercellular adhesion molecule 1 (ICAM-1), was observed on proximal tubular epithelial cells, indicating that these cells may play a role in antigen presentation. The cell biological experiments revealed the presence of the three mitotic fibroblast types (MFI-MFIII) and the three postmitotic types (PMFIV-PMFVI) in the cell culture. The number of fibroblasts in primary and passage-1 culture was increased sevenfold in cultures derived from kidneys with RIF (FKIF cells) in comparison to normal kidneys (NKF cells). FKIF cells show hyperproliferative growth and synthesize an increased amount of total collagen, especially types III and V. These cells express a protein, named “FIBROSIN”, which seems to be specific for FKIF cells. Further extended cell biological analyses are currently being performed to investigate interactions of tubular cells, lymphocytes, macrophages, and fibroblasts in order to shed more light on the pathomechanisms involved in fibrogenesis leading to renal interstitial fibrosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01649320
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  • 4
    Electronic Resource
    Electronic Resource
    Immunogenetics of glomerulonephritis (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. 822-824 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00190329
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  • 5
    Electronic Resource
    Electronic Resource
    Pharmacokinetics of torasemide and its metabolites in end-stage renal disease (1994)
    Springer
    European journal of clinical pharmacology 47 (1994), S. 157-159 
    Details
    ISSN: 1432-1041
    Keywords: Torasemide ; metabolites ; end-stage renal disease ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract The pharmacokinetics of torasemide, a new loop diuretic, as well as its active metabolites M1 and M3, and its inactive main metabolite, M5, were studied in 12 patients with end-stage renal failure during single i.v. (n=6) or single oral (n=6) dosing of 200 mg torasemide, and during chronic oral treatment for 9 days (n=12). The elimination half-life (t1/2) of torasemide was unchanged in renal failure, whereas t1/2 of the torasemide metabolites M1, M3, and M5 were markedly prolonged. However t1/2 as well as the area under the plasma level time curve of torasemide and its metabolites were unchanged during chronic compared to acute administration. The results of this study suggest that despite the increased half-life of torasemide metabolites M1, M3 and M5 in end-stage renal failure patients, no accumulation of the parent drug torasemide and its metabolites during chronic dosing is demonstrable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194966
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  • 6
    Electronic Resource
    Electronic Resource
    Renal effects of the new calcium channel blocking drug isradipine (1990)
    Springer
    European journal of clinical pharmacology 39 (1990), S. 333-335 
    Details
    ISSN: 1432-1041
    Keywords: Isradipine ; renal function ; calcium antagonist ; healthy volunteers ; side effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The acute effect of a single oral dose of isradipine 5 mg on blood pressure, renal haemodynamics, electrolyte excretion and plasma renin activity was studied in 10 healthy males. Isradipine did not produce a significant change in systolic or diastolic blood pressure, and glomerular filtration rate, renal plasma flow, renal vascular resistance, and urinary albumin excretion remained constant. There was a marked natriuretic and diuretic effect about 1–3 h after isradipine. Plasma renin activity showed a slight, insignificant increase 1 h after dosing. Uric acid clearance and β2-microglobulin excretion showed no significant changes, despite an increase in sodium clearance, suggesting an additional mechanism of action other than the proximal tubular natriuretic effect of isradipine in normotensive volunteers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315405
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  • 7
    Electronic Resource
    Electronic Resource
    Demonstration of a Na+-dicarboxylate cotransporter in bovine adrenocortical cells (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 860-864 
    Details
    ISSN: 1432-2013
    Keywords: Dicarboxylate transporter Succinate uptake Citrate pH-Dependency 2,3-Dimethylsuccinate Bovine adrenocortical cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Our study found the uptake of [14C]succinate into bovine adrenocortical cells to be sodium-dependent, inhibited by lithium, and to have an apparent K m of 146 µmol/l. Succinate uptake was inhibited by glutarate, fumarate, α-ketoglutarate, and maleate but not by 2,3-dimethylsuccinate or cis-aconitate, specific inhibitors of the basolateral Na+-dicarboxylate transporter of renal proximal tubule cells. Succinate uptake was highest at pH 6.0 and decreased with increasing pH. Transport of succinate was not significantly inhibited by citrate at pH 7.4 whereas at pH 6.0 inhibition of succinate uptake by citrate was small but significant. The affinity of the adrenal dicarboxylate transporter towards succinate ranges in between the low affinity of the renal luminal dicarboxylate transporter and the high affinity of the respective basolateral transporter. The pH dependency of succinate uptake and the missing inhibition by citrate at pH 7.4 differ from both the luminal and from the basolateral dicarboxylate transporters in kidney, liver, intestine, and placenta. These functional characteristics provide evidence for the existence of a Na+-dicarboxylate cotransporter in adrenocortical cells which may supply cholesterol metabolism with reducing substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900148
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  • 8
    Electronic Resource
    Electronic Resource
    Choline transport and its osmotic regulation in renal cells derived from the rabbit outer medullary thick ascending limb of Henle (1997)
    Springer
    Pflügers Archiv 434 (1997), S. 815-821 
    Details
    ISSN: 1432-2013
    Keywords: Key words Choline transport ; Organic osmolytes ; Osmoregulation ; Betaine ; TALH ; Rabbit kidney
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Organic osmolytes such as betaine and glycerophosphorylcholine (GPC) are of major importance concerning volume regulation of inner and outer medullary epithelial cells. Recently we demonstrated that the intracellular betaine content in rabbit kidney cells derived from the outer medullary thick ascending limb of Henle’s loop (TALH) is osmotically regulated by betaine synthesis. In this context it was our purpose to characterize the uptake of choline, a precursor of betaine and GPC. We found TALH cells to possess a specific choline transport system with a maximum velocity (V max) of 71 ± 12 pmol ·μl–1 cell water · min–1 and an apparent affinity (K m) of 155 ± 19 μmol · l–1. The uptake of choline was sodium independent and not electrogenic, but it was significantly reduced by the removement of chloride from the incubation medium. After long-term adaptation of TALH cells to a hyperosmotic medium (600 mosmol · l–1, osmolarity adjusted with NaCl or urea) a significant higher choline uptake rate was observed (V max: 166 ± 9 (NaCl), 96 ± 12 (urea) pmol ·μl–1 cell water · min–1). Our results suggest that the uptake of choline is due to higher intracellular requirements of choline under hypertonic conditions. Finally, an increase in the V max of the choline transport system may enable sufficient synthesis of betaine and GPC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050470
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  • 9
    Electronic Resource
    Electronic Resource
    Phänotypische Heterogenität kardialer Myxome (1987)
    Springer
    Journal of molecular medicine 65 (1987), S. 912-919 
    Details
    ISSN: 1432-1440
    Keywords: Heterogeneity of cardiac myxomas ; Immunohistology of cardiac myxomas ; Endothelial characteristics of cardiac myxomas ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Histogenesis of cardiac myxoma is still unclear. Beside endothelial cells a variety of different cell types were detected in this benign cardiac tumor. Cryostat sections of four myxomas were analysed in the indirect immunoperoxidase technique using monoclonal antibodies (MoABs) directed against MHC class I and II antigens, as well as different surface determinants specific for endothelial cells or monocytes/macrophages. Tumor cells forming cell clusters and blood vessel like structures differed in their expression of endothelial antigens suggesting cellular heterogeneity within single and different myxomas. Like in fetal cardiac tissue vascular channels rarely carried HLA-class II antigens. Tumor cells carrying antigens of monocytes/macrophages, as well as intracellular alkaline phosphatase of endothelial cells could represent subpopulations of an early differentiation stage. This analysis further supports previous hypothesis of an endothelial origin of myxomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01745502
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  • 10
    Electronic Resource
    Electronic Resource
    HLA-DR-MT matching improves graft survival rate in cadaver kidney transplantation (1983)
    Springer
    Journal of molecular medicine 61 (1983), S. 17-23 
    Details
    ISSN: 1432-1440
    Keywords: MHC ; Cadaver kidney transplantation ; Graft survival rate ; Blood transfusions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of prospective HLA-DR matching on the graft survival rate was investigated in a multicenter analysis of 85 transplants. Simultaneously in a retrospective analysis of graft outcome the importance of matching for MT-antigens MT1, MT2 and MT3 as a newly defined B-cell alloantigen system was evaluated. HLA-DR antigens and MT-specificities were determined on B-cells enriched by nylon-wool filtration using locally well characterised HLA-DR antisera and the antiserum set of the 8th International Histocompatibility Workshop (“discase set”) which allowed the definition of the HLA-DR specificities HLA-DR 1–9 and of the MT-antigens MT 1–3. HLA-DR matching showed a significantly improved graft outcome only in HLA-DR identical donor-recipient combinations. In 11 of 60 patients with one HLA-DR compatibility additional matching for two MT-antigens, however, improved the two year graft survival rate from 60% to 91%. Altogether 17 patients were matched for two MT-specificities with their kidney donor and showed a superior prognosis of 94% at two years compared to 53% or 17% of recipients with one or zero MT compatibility. Graft outcome in this patient group was also superior to that of HLA-DR identical or HLA-AB identical grafts. These data suggested that the MT-system rather than the HLA-DR antigens may be of critical importance in cadaver kidney transplantation. In addition a favorable influence of pretransplant blood transfusions on less HLA-DR matched grafts was confirmed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01484435
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