ISSN:
1573-5168
Schlagwort(e):
cDNA cloning
;
TSH regulation
;
thyroxine (T4)
;
triiodothyronine (T3)
;
negative feed-back
;
hypothalamus–pituitary gland–thyroid gland axis
;
GTH regulation
;
goldfish
;
glycoprotein family
;
molecular evolution
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A cDNA encoding the β subunit of thyrotropin (TSH) was isolated from a goldfish (Carassius auratus) pituitary gland cDNA library. By comparing the sequence with other teleost TSHβs, a signal peptide of 19 amino acids and a mature hormone of 131 amino acids were predicted for goldfish TSHβ subunits. The resulting putative mature hormone of 131 amino acids had well-conserved cysteine positions and a putative N-linked glycosylation site; homology was 51–67% with TSHβs from other teleosts, 38–43% with tetrapod TSHβs, but only 27 and 29% with goldfish GTH-Iβ and -IIβ, respectively. We also examined the effects of thyroid hormones (TH) and thiourea (TU, an inhibitor of TH production) treatments on TSH and GTH subunit gene expressions in the goldfish pituitary gland. After thyroxine (T4) treatment, circulating T4 concentration increased and TSHβ mRNA level decreased. Supressing the amount of circulating T4 and triiodothyronine (T3) by TU treatment increased the TSHβ mRNA level. Moreover, T4 replacement therapy (simultaneous treatment of both TU and T4) caused a high level of circulating T4 and a low level of circulating T3, and a decrease in the TSHβ mRNA level. Thus, changing levels of circulating TH exert a negative feedback on the level of TSH β subunit mRNA in goldfish in vivo. On the other hand, GTH subunit mRNA levels were not affected by changes in the levels of circulating TH.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1007884527397
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