Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Expression of HLA Class I and II (DR, DP and DQ) Determinants in Fetal and Postnatal Salivary Glands (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 34 (1991), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fetal (n=20)and postnatal (n = 40) parotid glands were examined by two-colour immunohisto-chemistry combining monoclonal and polyclonal antibody reagents lo study the expression of HLA class I and Il (DR. DP. and DQ). CD45 and CD3, The epithelium lacked class II during fetal life, whereas class I determinants appeared in some acini and most major ducts. Fetal vessels were positive for both class I and class II (mainly DR), suggesting constructive expression. Some class II-positive (DR 〉 DP DQ) histiocytic cells, scaltered CD45+ leucocytes, and very few CD3+ T cells were present in the fetal stroma. The epithelium remained DR-negative the first few weeks after birth, but brisk expression was seen subsequently, DP and DQ remained virtually negative in the epithelium throughout the first year. A slight postnatal increase of class II expression (DR 〉 DP 〉 DQ). along with an apparent decrease in class I. was observed in the endothelium. The number of class Il-positive histiocytic cells. CD45+ leucocytes and CD3+ T cells, as well as the proportion of presumably activated (DR+) T cells, increased a few weeks after birth. The local immune system hence seemed to be stimulated by extrinsic factors, but the overall number of T cells nevertheless remained small. Stimuli other than T cell-derived lymphokines, therefore, probably explained the brisk postnatal epithelial DR induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1991.tb01577.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Development of immune response markers in the trachea in the fetal period and the first year of life (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Pediatric allergy and immunology 4 (1993), S. 0 
    Details
    ISSN: 1399-3038
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Immunoglobulin (Ig)-producing cells. T cells (CD3) and epithelial expression of seeretory component (SC) and HLA class II determinants (DR, -DP, -DQ) were studied by immunohistochemistry in 16 fetal and 15 postnatal specimens from the tracheal wall. Small amounts of secretory component (SC) was present in the traeheal surface and gland epithelium in the fetal period and inereased towards term. A few IgM-, IgD- and IgG-producing cells were present in some fetal specimens but no IgA- and IgE-produeing cells were found. Only very few CD3 + T-cells were present in fetal specimens and intraepithelial T-cells were virtually absent until after birth. Premature infants that lived for 1 week had less SC epithelial expression than mature infants of the same age. The density of CD3 + T-cells. IgA-, IgM-producing cells as well as the epithelial SC expression increased rapidly after birth. Epithelial MHC class II expression was absent in fetal specimens. HLA-DR was detected on the apical border of the surface epithelium one week after birth and was extensively expressed throughout the remaining postnatal period. E-pithelial DP and PQ expression were virtually absent during this same period. These features probably reflect local activation of the immune system in response to environmental factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3038.1993.tb00059.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Changes in the concentration and distribution of immunoglobulin-producing cells in SIDS palatine tonsils (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Pediatric allergy and immunology 6 (1995), S. 0 
    Details
    ISSN: 1399-3038
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Seventeen sudden infant death syndrome (SIDS) cases and 9 controls, were examined immunohistochemically with regard to the presence of IgA-, IgM-, IgD, and IgG, as well as for thesubtypes IgG1, IgG2-, IgG3-, and IgG4-immunocytes. Differences in compartmentalization were also investigated. Differences were demonstrated between SIDS and controls in total number of IgG cells per 0.1 mm2 tissue area (median: 18.3, range: 12.3-30.2 versus median: 6.3, range: 2.0-14.6) (p〈0.01), and for IgA immunocytes (median: 3.9, range: 2.4-5.0 versus median: 1.5, range: 1.1-3.7) (p〈0.05), while no differences were demonstrated for IgM cells (median: 1.8, range: 1.2-3.3 versus median: 1.8, range: 0.7-5.6) or IgD cells (median: 1.9, range: 0.8-2.9 versus median: 1.6, range: 0.7-2.4). Differences were demonstrated between SIDS and control IgG plasma cells in all the four palatine tonsillar compartments; germinal centre (p〈0.01), mantle zone (p〈0.05), interfollicular area (p〈0.01) and reticular epithelium (p〈0.01). Furthermore, the number of IgA cells was higher in SIDS vs. controls in both the germinal centre (median: 1.4, range: 0.6-2.1 versus median: 0.6, range: 0.3-1.3) (p〈0.05) and in the interfollicular area (median: 2.2, range: 1.1-3.1 versus median: 0.5, range: 0.4-2.0) (p〈0.01). For IgM immunocytes, differences were demonstrated in the germinal centre (median: 1.0, range: 0.4-1.6 versus median: 0.4, range: 0.3-1.3) (p〈0.01) as well as in the germinal centre (median: 0.6, range: 0.5-0.8 versus median: 0.4, range: 0.3-0.7) (p〈0.01) and in the interfollicular area (median: 1.2, range: 0.8-1.6 versus median: 0.5, range: 0.5-0.7) (p〈0.01) in the IgD immunocyte group. The total number of IgG1- and IgG3-immunocytes were increased in SIDS (median 15.6, range: 5.3-58.9 versus median: 2.5, range: 1.5-8.4) (p〈0.01) and (median: 3.6, range: 0.4-8.6 versus median: 1.1, range: 0.4-1.3) (p〈0.01) respectively. Furthermore, significantly increased numbers of these two subclasses, were seen in all the four compartments in the SIDS cases. The palatine tonsillar immune system is stimulated in SIDS. Furthermore, the changes being predominantly in the germinal centres and interfollicular areas, are indicating a recent stimulation, and the IgG-subgroup response pattern makes a viral protein antigen the most likely stimulant.;
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3038.1995.tb00258.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Is partial deletion of the complement C4 genes associated with sudden infant death? (1994)
    Springer
    European journal of pediatrics 153 (1994), S. 287-290 
    Details
    ISSN: 1432-1076
    Keywords: Sudden infant death ; C4 deficiency ; Infections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The two C4 loci C4A and C4B in 61 cases of sudden infant death (SID), 93 living controls and 7 cases of infectious death were studied. In the SID group 13.1% showed deletion of the C4A gene, while 2.5% of the cases showed deletion of the C4B gene. This was not significantly different from neither the controls nor the infectious death group. We were not able to confirm that deletion of the C4B gene is associated with SID. However, in the SID group deletion of either the C4A or the C4B gene was associated with signs of infections prior to death (P=0.035). This observation may indicate that a proportion of SID victims are more vulnerable to infections than other infants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01954522
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Is the medium-chain acyl-CoA dehydrogenase G985 mutation involved in sudden infant death in Norway? (1995)
    Springer
    European journal of pediatrics 154 (1995), S. 166-167 
    Details
    ISSN: 1432-1076
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01991929
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Inverse relationship between beta-endorphin immunoreactivity in cerebrospinal fluid and nucleus tractus solitarius in sudden infant death (1994)
    Springer
    European journal of pediatrics 153 (1994), S. 381-386 
    Details
    ISSN: 1432-1076
    Keywords: Key words: Beta-endorphin – Cerebrospinal fluid – Nucleus tractus solitarius – Sudden infant death
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. In nucleus tractus solitarius (NTS) beta-endorphin (BEND) induces bradycardia and respiratory depression which have been reported to precede death in sudden infant death (SID). Of SID victims, 50% have elevated levels of beta-endorphin immunoreactivity (BENDI) in the cerebrospinal fluid (CSF), and 50% had undetectable levels. We therefore investigated the relationship of BENDI in the CSF to BENDI levels in the NTS area. This study included SID victims (CSF from n=47, brain stem from n=16), borderline SID victims (CSF and brain stem from n=2), sudden death in childhood (CSF and brain stem from n=1), and controls (CSF from n=32, brain stem from n=11). BEND in CSF and NTS area, after extraction, was measured by radioimmunoassay. High performance liquid chromatography was used for closer identification of BENDI. We found that the SID victims divided into two subpopulations, one having a relatively high BENDI level in CSF and one having no detectable level (P〈0.01). Furthermore, an inverse relationship was found between BENDI level in CSF and BENDI level in NTS area in the SID victims (P〈0.05). We conclude that increased BENDI level in CSF is associated with low BENDI level in the NTS area in 50% of SID victims. The low BENDI level in the NTS area may be due to increased release of BEND.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01956426
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Elevated beta-endorphin immunoreactivity in the cerebrospinal fluid in victims of sudden infant death correlates with hypoxanthine in vitreous humour (1993)
    Springer
    European journal of pediatrics 152 (1993), S. 935-938 
    Details
    ISSN: 1432-1076
    Keywords: Beta-endorphin ; Hypoxanthine ; Hypoxia ; SID
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Beta-endorphin (BEND) may induce respiratory depression. Elevated levels of beta-endorphin immunoreactivity (BENDI) in the CSF are found in children with apnoea and in about 50% of sudden infant death (SID) victims. Premortal hypoxia in SID victims has been indicated by elevated hypoxanthine (HX) levels in the vitreous humour (VH). In this study we correlated BENDI in CSF with HX in VH in SID victims (n=19) and controls (n=18). BEND in CSF was measured by RIA, and HPLC was used for identification of BENDI. HX in VH was measured by HPLC. All the SID victims had elevated levels of HX in VH. The BENDI in CSF divided the SID victims into two subpopulations (P〈0.01); one with undetectable levels (〈4.3 fmol/ml) (n=10) and one with high levels (160–400 fmol/ml) (n=9).In the SID subpopulation with high levels of BENDI in CSF,we found a correlation between BENDI in CSF and HX in VH (r=0.92). Control infants who died a stressful death, such as during heart operations (n=2), had high levels of BENDI in CSF and low levels of HX in VH. Controls who died of infections (n=11) had low levels of BENDI in CSF and elevated levels of HX in VH. Because hypoxia in itself does not increase BENDI in CSF, increased BENDI in CSF is probably not secondary to hypoxia but may be of aetiological significance. We therefore suggest that SID victims with high levels of BENDI in CSF, which correlate with the elevated levels of HX in VH, may die from premortal hypoxia possibly induced by BEND.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01957536
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Is partial deletion of the complement C4 genes associated with sudden infant death? (1994)
    Springer
    European journal of pediatrics 153 (1994), S. 287-290 
    Details
    ISSN: 1432-1076
    Keywords: Key words: Sudden infant death C4 deficiency – Infections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The two C4 loci C4A and C4B in 61 cases of sudden infant death (SID), 93 living controls and 7 cases of infectious death were studied. In the SID group 13.1% showed deletion of the C4A gene, while 2.5% of the cases showed deletion of the C4B gene. This was not significantly different from neither the controls nor the infectious death group. We were not able to confirm that deletion of the C4B gene is associated with SID. However, in the SID group deletion of either the C4A or the C4B gene was associated with signs of infections prior to death (P=0.035). This observation may indicate that a proportion of SID victims are more vulnerable to infections than other infants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01954522
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Immunohistochemistry of epithelial cell markers in normal and pathological colon mucosa (1980)
    Springer
    Histochemistry and cell biology 67 (1980), S. 7-21 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The purpose of this study was to examine whether formal-dehyde-fixed tissue may afford reproducible and reliable immunhistochemical results when carcinoembryonic antigen (CEA), secretory component (SC), and epithelial IgA are evaluated semiquantitatively in normal and pathological colon specimens. Proximate tissue samples were processed by routine formalin fixation and by a cold-ethanol fixation method, respectively, and the immunofluorescence intensities obtained for the three antigens were scored. After formalin fixation SC and epithelial IgA were generally undetectable and also the staining for CEA was markedly reduced compared with that seen after ethanol fixation. Significant antigenic “unmasking” was obtained by enzyme treatment of the formalin-fixed tissue sections-resulting in enhanced staining for SC and epithelial IgA but not consistently so for CEA. With this modification scores from duplicate tissue samples processed by the two methods showed significant correlations for all the three epithelial markers; small amounts of CEA and epithelial IgA, and especially SC, nevertheless remained undetectable after formalin fixation. This result should be taken into account when epithelial markers are applied in studies of premalignant lesions of the colon where minor changes in the antigen pattern may be of diagnostic importance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490083
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Evaluation of nine different fixatives (1984)
    Springer
    Histochemistry and cell biology 81 (1984), S. 213-219 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An artificial substrate was developed for quantitative testing of the ability of various fixatives to preserve the reactivity of IgG and IgA isotypes (γ and α chains) and the secretory component (SC) of secretory IgA as model antigens. Polymerized normal rabbit serum was used as matrix and defined amounts (10–0.1 g/l) of antigen were incorporated into it by diffusion before fixation and paraffin embedding. The various fixatives comprised alcohol, routine formalin, glutaraldehyde(1%)-formalin, Baker's formol calcium, formol sublimate, acetic acid(2%)-formol saline, Bouin's fluid, Susa fixative, and carbodiimide. The detection sensitivity afforded by these fixatives was defined as the immunofluorescence staining end point. Compared to the reference value obtained with alcohol (γ and α chains, 0.06 g/l of IgG and IgA; SC, 0.12 g/l of colostral IgA), an antigen concentration at least 8 times higher was necessary for detection with most of the cross-linking fixatives. Bouin's and Susa fixatives were peculiar in that they required more than 150 times higher antigen concentration for detection of IgG but only 3–8 times higher for IgA. The determined sensitivities were compared with the immunofluorescence performance results obtained on human tissues prepared with the same fixatives; excepting carbodiimide (which produced unacceptable autofluorescence of the substrate matrix) a remarkably good correlation was found with regard to IgG- and IgA-producing cells (especially of the former isotype) and secretory epithelium (IgA and SC). However, the latter result depended on pronase treatment of the tissue sections to unmask epithelial antigens. It was concluded that detection sensitivity as determined with this artifical substrate may give a good idea about the immuno-histochemical performance obtained on tissue prepared with the actual fixative; but the degree of antigenic masking in the various tissue compartments has to be taken into account if the comparisons are to be meaningful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495630
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...