ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— Proteins of the brain extracts of 85 individual pigeons (Columba livia) were mapped by two-dimensional gel electrophoresis. The method is a modification of O'Farrell'S technique and separates proteins first by charge and then by molecular weight. There were three proteins, A, B and D which had each a variant form. The positions of these six proteins on the gel corresponded to the following pH values and molecular weight values: protein A1, 6.4/43,000; A2, 6.6/43,000; B1, 5.7/41,000; B2, 5.8/40,000; D1, 6.2/22,000; D2, 6.2/21,000. The variants are genetically determined, since protein A, B and D each occurred in three phenotypes (A1, A1A2 and A2; B1, B1B2 and B2; D1, D1D2 and D2) corresponding to the three possible genotypes. From the observed frequencies of the phenotypes the following allele frequencies were calculated: allele A1, 72%; A2, 28%; B1, 15%; B2, 85%; D1, 74%; D2, 26%. A fourth protein named C occurred in four different forms (C1, 7.2/37,000; C2, 7.2/36,000; C3, 7.1/37,000; C4, 7.1/36,000) and six phenotypes (C1, C1C2, C2, C1C3, C2C3 and C4C3). This polymorphism is also interpreted as being genetically determined. The four alleles coding for the four protein C forms had the following frequencies: allele C1, 62%; C2, 27%; C3, 10.5%; C4, 0.5%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1979.tb05248.x
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