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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food quality 18 (1995), S. 0 
    ISSN: 1745-4557
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Single-cell protein (SCP) produced from a novel thermotolerant methylotrophic Bacillus sp. KISRI-TM IA was fed to groups of 10 male and 10 female Wistar rats at levels of 0, 10, 20, and 30 percent of total diets. The feeding of KISRI-TM IA did not adversely affect growth, feed intake or hematological values of rats. Water intake of male rats decreased with increasing level of SCP in the diet. Urinary pH showed dose-dependent reductions in both sexes. Plasma alkaline phosphatase and alanine aminotransferase were significantly (P〈.05) reduced by increasing levels of dietary SCP. However, no significant (p〉.05) abnormalities were observed in the weights or histopathological appearances of the organs. It was concluded that the KISRI-TM 1A SCP could serve as a potential dietary ingredient at levels of up to 30% for poultry, young lambs and calves.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 5 (1989), S. 149-159 
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Résumé Quatre bactéries méthylotrophes, isolées à l'Institut pour la Recherche Scientifique du Kuweit, se sont révélées capables de croître sur méthanol comme seule source de carbone à une température maximum de 44°C. Des études intensives en chémostat de variation des concentrations tant en macro-qu'en micro-éléments, ont permis d'obtenir une composition optimum du milieu. Diverses expériences en milieu non renouvelé et en chémostat ont été effectuées à différents pH, températures, taux de dilution et concentration en méthanol. Les résultats ont montré un optimum de pH vers 6.8, de température entre 37 et 40°C, de taux de dilution de 0.2 à 0.3 h−1. Le méthanol s'est révélé inhibiteur aux concentrations supérieures à 20 g l−1. La performance des quatres bactéries dans les conditions du chémostat se sont révélées très semblables. Les expériences de fermentátion en chémostat, utilisant le milieu optimum à 40°C, pH 6.8, taux de dilution de 0.2 h−1 et à 10 g de méthanol par litre dans l'alimentation ont produit un coefficient de rendement en biomasse de 0.42 à 0.44 g de cellules par g de méthanol contenant de 78 à 79% de protéines, de 58 à 62% d'acides aminés totaux et de 10 à 11.5% d'acides nucléiques. En conclusion, les quatre bactéries méthylotrophes présentent une bonne potentialité pour être utilisées dans la production de protéine uni-cellulaire.
    Notes: Summary Four methylotrophic bacteria, isolated at the Kuwait Institute for Scientific Research, were able to grow on methanol as the only carbon source at a maximum temperature of 44°C. An optimized medium composition was obtained through intensive chemostat studies varying both macro- and micro-element concentrations. Various batch and chemostat experiments were carried out at different pH, temperature, dilution rate and methanol concentrations. The results showed optimum pH around 6.8, at temperatures of 37 to 40°C, dilution rate 0.2–0.3 h−1 and methanol was found to be inhibitory at concentrations above 20 g l−1. The performance of all four bacteria under chemostat conditions was similar. Chemostat fermentation experiments using the optimized medium at 40°C, pH 6.8, dilution 0.2 h−1 and 10 g methanol/l in the feed gave a biomass yield coefficient of 0.42–0.44 g/g methanol, 78–79% crude protein content, 58–62% total amino acid content and 10–11.5% nucleic acid content. In conclusion all four methylotrophic strains has good potential for use in the production of single-cell protein.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 5 (1990), S. 39-45 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The introduction of more imaginative enrichment and isolation procedures has permitted the isolation of pure cultures of thermotolerant methylotrophic bacteria, a group that was previously unknown. One potential application for such bacteria is in the aerobic biotreatment of petrochemical industry wastewaters at elevated temperatures. Here, the growth and biooxidation characteristics of one such bacterium, Bacillus sp. NCIB 12522, in co-culture with a Gram-negative thermophilic non-methylotrophic solvent utilizing bacterium, NA 17, on a mixture of methanol, ethanol and isopropanol, under both steady and transient state continuous flow culture conditions are reported. The results indicate that at dilution rates 〈0.2 h−1 effective biooxidation can be achieved at temperatures between 50° and 57 °C. As a result of step increases in bioreactor feed concentrations, the fraction of the methylotroph present in the co-culture changed according to whether the methanol or the ethanol concentrations were increased, but when isopropanol was increased, no change in the methylotroph fraction occurred between the initial and final steady states.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 30 (1989), S. 408-414 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In large industrial scale bioreactors process cultures are frequently exposed to supperoptimal temperatures for short intervals of time. Data concerning the physiologica effects of such exposures are scant. Here, experiments involving short term heat shocks on two bacteria, Klebsiella pneumoniae, a non-fastidious mesophile growing on glucose and Bacillus sp. NCIB 12522, a fastidious thermotolerant methylotroph growing on methanol, are described. Markedly different response patterns for the two bacteria are evident, clearly indicating not only the dangers of making generalizations with respect to the effects of superoptimal temperatures on growing bacterial cultures but also, the significance of scale related segregation phenomena such as wall growth.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 73-78 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of temperature on chemostat cultures of a thermotolerant, methylotrophic Bacillus ap. are reported. In spite of the relative fastidiousness of this bacterium, overall response to a range of growth temperatures is predictable. However, anomalous results with respect to biomass yield coefficients are evident, suggesting complex and extensive endogenous and exogenous activities under some growth conditions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is expressed as crude and true protein content, moisture and ash content, mineral composition of the ash, and amino acids profiles. The most suitable harvesting procedures were found to be those using a mild heat-acid or acid-heat treatment. Both of these procedures gave high recovery efficiencies and suitable product nutrition.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 20 (2000), S. 335-350 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: During the 20th century, important relationships developed between the oil industry and both microbiological and biotechnological research. Basic microbiological research has played an important role in both the exploration and production sectors of the oil industry, but as the maturity of the industry has progressed, such contributions have been relegated with respect to their importance. With respect to refining and petrochemicals manufacture, process routes have been extensively researched, but only rarely have the biotechnological solutions developed satisfied the economic criteria that resulted in major investment. In fact, situations exist where investment has occurred, but project life was unrealistically short, suggesting a need for extreme caution when evaluating biotechnological processes for the oil industry. However, as far as engineered processes for both biotreatment and bioremediation are concerned, the fundamental research that has underpinned other areas of hydrocarbon microbiology will finally prove to be of both technical and economic value, in ensuring that the essential needs of treatment, rather than disposal, and restoration, rather than environmental destruction, can be satisfied by the oil and other industries involved in both geochemical manipulation and natural resource exploitation.
    Type of Medium: Electronic Resource
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