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  • 1
    Electronic Resource
    Electronic Resource
    Stability of biotransformation capacity in Digitalis lanata cell cultures after cryogenic storage (1983)
    Springer
    Plant cell reports 2 (1983), S. 273-276 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Suspension cultures of Digitalis lanata were stored at the temperature of liquid nitrogen. After thawing, viable cultures were recovered which showed a growth rate equal to that of untreated cells. The capacity of Digitalis cells to transform β-methyldigitoxin to β-methyldigoxin remained unchanged after cryostorage. This was shown in 300-ml shake cultures as well as in 20-1 bioreactors. From these results it is clear that cryostorage is a suitable method for preserving cell lines with specific capacities to produce compounds of medical and industrial importance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269159
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  • 2
    Electronic Resource
    Electronic Resource
    Cultivation of cell cultures of Berberis wilsonae in 20-l airlift bioreactors (1985)
    Springer
    Plant cell reports 4 (1985), S. 220-223 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Suspension cultures of Berberis wilsonae produce 4 berberine-type alkaloids: berberine, palmatine, columbamine and jatrorrhizine. In particular the formation of the phenolic alkaloids columbamine and jatrorrhizine and of berberine proves to be dependent on the concentration of dissolved oxygen. With higher aeration rates, berberine and jatrorrhizine yields can be increased considerably. Thus we reached an alkaloid yield of more than 3 g × 1−1 with 50% dissolved oxygen tension in the medium. As far as we know this is one of the best results in fermenting of alkaloid-producing cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269294
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    High forskolin production in hairy roots of Coleus forskohlii (1998)
    Springer
    Plant cell reports 17 (1998), S. 457-459 
    Details
    ISSN: 1432-203X
    Keywords: Key words Forskolin ; Coleus forskohlii ; Hairy root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy roots of Coleus forskohlii were induced by infection with the Agrobacterium rhizogenes MAFF 03-01724 strain. Growth and forskolin production of two hairy root clones cultured in various liquid media were examined. Hairy root clone B9 grew well in woody plant liquid medium and showed a high forskolin yield (ca. 1.3 mg/ 100 ml flask) after 5 weeks of culture. The time course of growth and forskolin production of the clone B9 cultured in woody plant liquid medium was also examined. Rapid growth started at week 2 and continued until week 5. The highest forskolin yield (ca. 1.6 mg/100 ml flask) was obtained at week 5. Productivity was much higher than that previously reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050425
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  • 4
    Electronic Resource
    Electronic Resource
    Immobilization of digitoxin 12β-hydroxylase, a cytochrome P-450-dependent enzyme from cell cultures of Digitalis lanata EHRH (1987)
    Springer
    Plant cell reports 6 (1987), S. 200-203 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Attempts were made to immobilize digitoxin 12β-hydroxylase, a membrane-bound, cytochrome P-450-dependent monooxygenase from cell cultures of Digitalis lanata. The optimum procedure was the entrapment of microsomes in 2% alginate by crosslinking the polysaccharide chains with CaCl2. After the immobilization of the enzyme about 70% of its activity was retained. The kinetic data such as the pH optimum and the optimum substrate concentrations were identical for the immobilized enzyme and freely suspended microsomes. Using β-methyldigitoxin as a substrate enzyme activity could be observed for more than 20 h. A continuous flow system for immobilized digitoxin 12β-hydroxylase is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268479
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  • 5
    Electronic Resource
    Electronic Resource
    Eugenol from normal and transformed root cultures of Coluria geoides (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 273-276 
    Details
    ISSN: 1573-5044
    Keywords: essential oil ; hairy roots ; Rosaceae ; normal roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transformed root cultures of Coluria geoides Ledeb. were established with the use of Agrobacterium rhizogenes LBA 9402. Both normal and transformed root cultures were investigated for their growth and yield of eugenol. Normal roots were grown in B5 medium-supplemented with 0.2 mg l-1 of kinetin and 0.2 mg l-1 of 1-naphthaleneacetic acid (NAA). Hairy roots grew well in hormone-free B5 medium. Both hairy roots and normal roots produced glycosidic bound eugenol. as with the roots of intact plants, eugenol was the main component of the total essential oils obtained from hairy root and normal root cultures. The yield of eugenol from normal roots was 0.1–0.25% of the dry wt. and depended on the development stage of the culture. Yield of eugenol from hairy roots was 0.08–0.1% of the dry wt. NAA modified the hairy root morphology and influenced the yield of eugenol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043642
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  • 6
    Electronic Resource
    Electronic Resource
    Natural product formation by plant cell biotechnology (1995)
    Springer
    Plant cell, tissue and organ culture 43 (1995), S. 199-205 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This short review tries to compile some results, which show the importance and the potential of plant cell and tissue cultures for a biotechnological production of natural products. On the other hand, it can not be denied, that a real breakthrough of this technique has not yet been achieved. The problems involved and possible ways to overcome these will be discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00052176
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