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  • 1
    Electronic Resource
    Electronic Resource
    An assessment of growth yields and energy coupling inDesulfovibrio (1978)
    Springer
    Archives of microbiology 117 (1978), S. 21-26 
    Details
    ISSN: 1432-072X
    Keywords: Desulfovibrio ; Growth yields ; Anaerobic ; Bioenergetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The yield coefficients forDesulfovibrio vulgaris andD. gigas varied with the electron donoracceptor combinations and with the bacterial strain. The only evidence for electron transport coupled formation of adenosine triphosphate (ATP) was with sulfate as the electron acceptor. WithD. vulgaris the ATP formation coupling to electron flow with pyruvate oxidation was 1:4 electrons and with lactate oxidation was 1:8 electrons. WithD. gigas these ratios were 1:8 electrons and 1:16 electrons for the oxidation of pyruvate and lactate. The clearest resolution of energy coupling was withD. vulgaris growing on formatesulfate medium where 2 ATP appear to be formed with the transfer of electrons from formate to adenosine phosphosulfate and one ATP with the transfer of electrons from formate to sulfite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689346
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  • 2
    Electronic Resource
    Electronic Resource
    Protoporphyrinogen oxidation coupled to nitrite reduction with membranes from Desulfovibrio gigas (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 61 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The oxidation of protoporphyrinogen by membranes from Desulfovibrio gigas with nitrite as the electron acceptor proceeds at the ratio of 1 mol protoporphyrin produced for each mol of nitrite reduced. Coupled to the protoporphyrinogen-nitrite reaction is the esterification of ortho-phosphate with a P/2e− value of 0.92. Pentachlorophenol, 3 × 10−5 M, and carbonyl cyanide m-chlorophenyl hydrazone, 3 × 10−6 M, serve as uncouplers of phosphorylation while rotenone, 9 × 10−6 M, and hydroxyquinoline-N-oxide, 0.1 × 10−6 M, inhibit electron flow from protoporphyrinogen oxidase to nitrite reductase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03553.x
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  • 3
    Electronic Resource
    Electronic Resource
    Transformation of selenate and selenite to elemental selenium byDesulfovibrio desulfuricans (1995)
    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 329-336 
    Details
    ISSN: 1476-5535
    Keywords: Selenium ; Desulfovibrio ; Sulfate-reducing bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Desulfovibrio desulfuricans (DSM 1924) can be adapted to grow in the presence of 10 mM selenate or 0.1 mM selenite. This growth occurred in media containing formate as the electron donor and either fumarate or sulfate as the electron acceptor. As determined by electron microscopy with energy-dispersive X-ray analysis, selenate and selenite were reduced to elemental selenium which accumulated inside the cells. Selenium granules resulting from selenite metabolism were cytoplasmic while granules of selenium resulting from selenate reduction appeared to be in the periplasmic region. The accumulation of red elemental selenium in the media following stationary phase resulted from cell lysis with the liberation of selenium granules. Growth did not occur with either selenate or selenite as the electron acceptor and13C nuclear magnetic resonance indicated that neither selenium oxyanion interfered with fumarate respiration. At 1 μM selenate and 100 μM selenite, reduction byD. desulfuricans was 95% and 97%, respectively. The high level of total selenate and selenite reduced indicated the suitability ofD. desulfuricans for selenium detoxification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569947
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of iron(III) analogs on growth and pseudobactin synthesis in a chromiumtolerantPseudomonas isolate (1991)
    Springer
    BioMetals 4 (1991), S. 211-216 
    Details
    ISSN: 1572-8773
    Keywords: Pseudobactin siderophore ; Iron metabolism ; Chromium ; Gallium ; Scandium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The growth and siderophore production of a fluorescentPseudomonas species isolated from soil contaminated with chromium was found to be influenced by the presence of trivalent cations. Overproduction of pseudobactin occurred when the isolate was grown in media containing 1 mM Cr(III) under ironlimited conditions but not when Fe(III) was added at 10 μM Pseudobactin synthesis was derepressed in iron-limited cultures containing 1 mM Sc(III) or Y(III), examples of group III-B elements. We found that AI(III), Ga(III) or In(III), representative metals from group III-A, repressed synthesis of pseudobactin under iron-deficient conditions. Analogs of Fe(III) were found to inhibit growth of thePseudomonas isolate in iron-limited media and the trivalent metals listed in order of decreasing toxicity were as follows: Ga 〉 In 〉 Sc 〉 Cr 〉 Y 〉 Al. The inhibition of growth by 1 mM In(III), Sc(III) and Ga(III) was greater during iron-limited growth than in media containing 10 μM Fe(III). These data show that, although the metal analogs of Fe(III) have similar chemical and physical characteristics, the physiological response of the fluorescent pseudomonad when grown in the presence of these metals varied markedly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01141183
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  • 5
    Electronic Resource
    Electronic Resource
    Ferrochelatase activity inAzospirillum brasilense with reference to the influence of metal cations (1989)
    Springer
    BioMetals 2 (1989), S. 31-35 
    Details
    ISSN: 1572-8773
    Keywords: Heme synthesis ; Iron metabolism ; Membranes ; Cation inhibition ; Metalloporphyrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Ferrochelatase in membrane preparations fromAzospirillum brasilense displayed an activity of 2.17 μmol protoheme formed · h−1 · mg protein−1 which is 10-fold greater than previous reports for other bacteria. This ferrochelatase showed an apparentK m of 20.9 μM for Fe2+, a pH optimum of 6.0–6.5, and stimulation by oleic or stearic acids. Co2+, Cu2+ and Zn2+ inhibited the incorporation of Fe2+ into protoporphyrin IX while Ni2 and Mg2+ had no effect on protoheme synthesis. Activity with Fe2+ and mesoporphyrin IX was less than with protoporphyrin IX but deuteroporphyrin IX produced the highest rate of protoheme synthesis. The membrane fraction containing ferrochelatase activity was found to insert Cu2+, Ni2+, Zn2+ and Co2+ enzymatically into protoporphyrin IX to produce metalloporphyrins. Cu2+ incorporation into protoporphyrin IX proceeded at a rate greater than with Fe2+ and theK m for Cu2+ was 21.9 μM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116198
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  • 6
    Electronic Resource
    Electronic Resource
    Removal of U and Mo from water by immobilized Desulfovibrio desulfuricans in column reactors (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 60 (1998), S. 88-96 
    Details
    ISSN: 0006-3592
    Keywords: molybdenum ; uranium ; immobilized cells ; dissimilatory reduction ; Desulfovibrio ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Intact cells of Desulfovibrio desulfuricans were immobilized in polyacrylamide gel and used to remove soluble U and Mo from water by enzymatically mediated reduction reactions in column reactors. Formate or lactate served as the electron donor and oxidized U(VI) and Mo(VI) species served as electron acceptors. Greater than 99% removal efficiencies were achieved for both metals with initial concentrations of 5 mg/L U and 10 mg/L Mo. Hydraulic residence times in the columns were between 24 and 36 h. Sulfate concentrations as high as 2000 mg/L did not inhibit reduction of U or Mo in the columns. However, nitrate inhibited uranium reduction at concentrations near 50 mg/L and inhibited molybdenum reduction at concentrations near 150 mg/L. The results indicate that enzymatic reduction of U and Mo by immobilized cells of D. desulfuricans may be a practical method for removing these contaminants from solution in continuous-flow reactors. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 88-96, 1998.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Environmental scanning electron microscope (ESEM) evaluation of crystal and plaque formation associated with biocorrosion (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 25 (1993), S. 429-433 
    Details
    ISSN: 1059-910X
    Keywords: Biocorrosion ; Sulfate-reducing bacteria ; Biofilm ; Desulfovibrio ; Electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The biofilm attributed to Desulfovibrio vulgaris growing in the presence of ferrous metals was examined with an environmental scanning electron microscope. This novel microscope produced images of iron sulfide colloids and other iron containing structures that had not been reported previously. A plaque composed of iron sulfide enveloped the surface of the corroding metal while crystals containing magnesium, iron, sulfur, and phosphorus were present in the culture where corrosion was in progress. A structure resembling the tubercule found in aerobic corrosion was observed on stainless steel undergoing biocorrosion and the elements present in this structure included sulfur, iron, chloride, calcium, potassium, and chromium. © 1993 Wiley-Liss, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070250513
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