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  • 1
    Electronic Resource
    Electronic Resource
    Low Water Absorption Copolymers - Nonstaining Superior Processing Type (1948)
    s.l. : American Chemical Society
    Industrial & engineering chemistry 40 (1948), S. 2384-2387 
    Details
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ie50468a032
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Micro-size polyacrylamide gel electrophoresis system (1987)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 58 (1987), S. 1736-1742 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The development and characterization of a micro-size two-dimensional polyacrylamide gel electrophoresis system is described. Some of the techniques which have evolved with use of the system are also discussed. This apparatus has unique features which provide advantages over other small scale units. Up to ten first- and second-dimension gels can be processed simultaneously with excellent resolution of protein regions. Consistent reproducibility is possible from protein samples as small as 400 ng and individual protein regions as small as 1 pg can be visualized by silver staining of the two-dimensional gels. Similar sensitivities are achieved in autoradiographs of 3H-labeled proteins extracted from the nuclei of cultured cells. The application of this system in conjunction with flow cytometric examination of nuclear DNA and electrostatic cell sorting of specific cell nuclei to provide homogeneous sample populations, allows subtle variations in isotope incorporation in proteins to be detected; whereas many times in generalized tissue samples these changes are masked. Also, these techniques elucidate the effects of external stimuli (chemicals, drugs, or environment) on protein synthesis and phosphorylation for analyses and comparison. Fabrication drawings are available upon request.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1139377
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Development of a Better Processing GR-S (1946)
    s.l. : American Chemical Society
    Industrial & engineering chemistry 38 (1946), S. 1246-1249 
    Details
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ie50444a014
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Histone variants, H1subtypes and other chromatin proteins as biomarkers of age and caloric restriction in rats (1993)
    Springer
    Age 16 (1993), S. 97-103 
    Details
    ISSN: 1574-4647
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The synthesis and phosphorylation levels of basic chromatin proteins from cell cycle phases of dividing bone marrow cells in Fischer 344 rats were investigated by gel electrophoresis. Two groups of rats, young ad libitum (Y/A 3 mo.) and old ad libitum (O/AL 28 mo.), had free access to rat chow, and a third group of old rats was maintained on a calorie-restricted intake (O/CR 28 mo.). Histone H1 subtypes a, b and c from combined S+G2 phase revealed highest amounts of [3H] lysine incorporation in Y/AL, lowest in O/AL, and intermediate levels in O/CR animals, while subtypes e and I° were highest in O/AL, lowest in Y/AL, and mid level in O/CR. The 32p-orthophosphate incorporation of these subtypes followed a parallel scenario as with lysine. Histone H4 variants from S phase demonstrated the highest levels of lysine labeling in O/AL, lowest in Y/AL and intermediate levels in O/CR. The H1 variants were similar in synthesis patterns to H4 variants but less dramatic. Minor histone variants M2, M3 and M4 manifested most synthesis in O/AL, least in Y/AL and mid levels in O/CR. Conversely, M1 synthesis was high in Y/AL, low in O/AL and modest in O/CR. Ubiquitin followed a similar pattern in lysine labeling to M1 and A24 which was inversely proportional to ubiquitin. The metabolic activity of these basic proteins in O/CR exemplifies a condition characteristic of Y/AL animals modulated by age and diet dependent factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02436141
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  • 5
    Electronic Resource
    Electronic Resource
    Lighting regimen dominates interacting meal schedules and synchronizes mitotic rhythm in mouse corneal epithelium (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 180 (1974), S. 47-52 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Three groups of mice were standardized to a light-dark cycle with light from 0600 to 1800. One group was fed ad lib; but the other two had access to food for only four hours a day, one during the first part of the light phase and the other beginning at its middle. Two other groups were subjected to a reversed light-dark cycle (light from 1800 to 0600); one of these had access to food for four hours during the first part of the dark phase and the other for four hours beginning at its middle. All the mice previously had been adjusted gradually over a three-week period to these feeding schedules, and then they were maintained on the precise routine described for an additional two weeks. After standardization was completed, subgroups of mice were killed at three-hour intervals over a single 24-hour period. Corneas were removed and prepared, and the mitotic index in the epithelium was evaluated.In all five groups a high-amplitude circadian rhythm was found for the mitotic index, but in all cases this rhythm remained synchronized to the light-dark cycle; only small changes in the phasing of the rhythm resulted from the restricted feeding. These results are contrary to what has been found for a number of other rhythmic variables which do synchronize to such feeding schedules.The findings dispel the misconception that all body functions react in the same fashion to different synchronizors and emphasize that one must not generalize about the effects of feeding or lighting.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091800106
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    Paper (German National Licenses)
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