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  • 1
    Electronic Resource
    Electronic Resource
    Two-stage Nested PCR Effectiveness for Direct Detection of Vibrio vulnificus in Natural Samples (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 64 (1999), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The nested primers designed to amplify a 222-base pair portion of the hemolysin gene, vvhA, were specific for all V. vulnificus strains tested. The nested PCR amplification, coupled with direct extraction of template DNA, revealed improved sensitivity sufficient for detection of 1 to 10 CFU V. vulnificus in 1 mL of seafood homogenates, and eliminated the need for enrichment culturing. Thereby, the nested PCR method achieved a broader applicability, making it effective for extensive use in identification of the pathogen in natural samples such as raw seafoods, seawater and sediments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1999.tb09882.x
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  • 2
    Electronic Resource
    Electronic Resource
    Optical study of ZnSexTe1−x alloys using spectroscopic ellipsometry (2000)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 2997-2999 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We report pseudodielectric function data 〈ε〉=〈ε1〉+i〈ε2〉 of ZnSexTe1−x samples grown on GaAs substrates. The data were obtained from 1.5 to 6.5 eV using spectroscopic ellipsometry. Critical-point parameters were obtained by fitting model line shapes to numerically calculated second-energy derivatives of 〈ε〉. The bowing parameters of E0, E1, and E1+Δ1 were determined and were comparable to that of E0 quoted from the literature. We observed a monotonic increase of the linewidth of the E1 gap up to x=0.85, whereas that of E1+Δ1 showed a maximum value near x=0.5. We attribute this anomalous broadening of the E1 gap to sample microstructures developed in the low-Te composition alloys. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1323999
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  • 3
    Electronic Resource
    Electronic Resource
    Ball material dependence of spinning rotor gage residual drag
    Amsterdam : Elsevier
    Vacuum 42 (1991), S. 897-900 
    Details
    ISSN: 0042-207X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0042-207X(91)90554-V
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  • 4
    Electronic Resource
    Electronic Resource
    Persistent photoconductivity in hydrogenated amorphous silicon
    Amsterdam : Elsevier
    Solid State Communications 59 (1986), S. 177-181 
    Details
    ISSN: 0038-1098
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0038-1098(86)90204-8
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  • 5
    Electronic Resource
    Electronic Resource
    Power-law dependence of persistent photoconductivity on exposure time in compensated a-Si : H
    Amsterdam : Elsevier
    Solid State Communications 86 (1993), S. 589-592 
    Details
    ISSN: 0038-1098
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0038-1098(93)90145-D
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of cinnarizine on IgE antibody-mediated experimental allergic reactions in guinea pigs (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 42 (1987), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The anti-allergic activity and mechanism of cinnarizine was investigated in guinea pigs. Nifedipine, a calcium antagonist, and tranilast, a potent, orally active anti-allergic agent, were used as comparative drugs. 1) Cinnarizine protected against fatal systemic anaphylactic shock in guinea pigs passively sensitized with IgE antibody. Cinnarizine reduced many of the features of severe respiratory disorders. Nifedipine and tranilast showed similar effects. 2) Cinnarizine and nifedipine inhibited the contractile response to antigen of sensitized tracheal smooth muscle when the challenge was carried out at low antigen concentrations. Tranilast showed a tendency to inhibit the antigen-induced contraction of tracheal smooth muscle. 3) Cinnarizine and nifedipine inhibited Ca-induced contraction in potassium-depolarized tracheal smooth muscle, tranilast had no effect. 4) Cinnarizine showed antagonistic action to the contraction by histamine or leukotriene D4 (LTD4) of tracheal muscle. Nifedipine showed similar antagonistic action, although its potency is lower than cinnarizine. Tranilast showed slight antagonistic action to LTD4. 5) Antigen-induced release of histamine and slow reacting substance of anaphylaxis (SRS-A) from sensitized lung tissues was inhibited by nifedipine and tranilast but not by cinnarizine. 6) The release of histamine and SRS-A from lung tissues by calcium ionophore A23187 was inhibited by nifedipine and tranilast but not by cinnarizine. These results suggest that the anti-allergic action of cinnarizine is mainly due to the antagonistic action to allergic mediators and not by interfering with die release of mediators. Cinnarizine's mechanism seems to be related to its antagonistic action to Ca in smooth muscle, but not to the transport of Ca in releasing the anaphylactic chemical mediators in mast cells and other target cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1987.tb02371.x
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  • 7
    Electronic Resource
    Electronic Resource
    Fracture of a ductile layer constrained by stiff substrates (2001)
    Oxford, UK : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 24 (2001), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A combined experimental and theoretical analysis of the fracture behaviour of brass/solder/brass sandwich specimens is conducted. First, the theories of interfacial fracture initiation for a ductile layer sandwiched between elastic substrates are reviewed. The fracture behaviours are then reported of brass/solder/brass sandwich specimens under various mode mixities. Additionally, the effects of solder layer thickness h and specimen lateral thickness t are presented. The effects of mode mix and plastic constraint on the failure mechanism and toughness are analysed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-2695.2001.00371.x
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  • 8
    Electronic Resource
    Electronic Resource
    Actual half-life of alpha-fetoprotein as a prognostic tool in pediatric malignant tumors (1997)
    Springer
    Pediatric surgery international 12 (1997), S. 599-602 
    Details
    ISSN: 1437-9813
    Keywords: Key words Actual half-life ; Alpha-fetoprotein ; Malignant germ-cell tumor ; Hepatic malignancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a retrospective study, the prognostic value of monitoring the decay of alpha-fetoprotein (AFP) was assessed. Serum AFP was determined serially in 18 children with malignant germ-cell or hepatic tumors: 7 endodermal sinus tumor, 3 embryonal carcinoma, 5 malignant teratoma, 2 hepatoblastomas, and 1 hepatocellular carcinoma. The actual half-life (AHL) of AFP was computed after surgical resection of the tumor. In group 1, which had complete resection and no recurrence during follow-up (n = 13), the AHL of AFP was 4.0 ± 0.9 days. In group 2, which had incomplete resection or recurrence during follow-up (n = 5), the AHL of AFP was 24.8 ± 20 days, significantly longer than that of group 1 (P = 0.0026). The increased AHL of AFP indicated residual active tumor after surgical resection. The AHL of AFP may be more sensitive than serial monitoring of AFP in detecting preclinical recurrence after surgical resection of AFP-secreting tumors. Treatment strategies can be based on AFP clearance, and prospective clinical trials are warranted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01371908
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  • 9
    Electronic Resource
    Electronic Resource
    Actual half-life of alpha-fetoprotein as a prognostic tool in pediatric malignant tumors (1997)
    Springer
    Pediatric surgery international 12 (1997), S. 599-602 
    Details
    ISSN: 1437-9813
    Keywords: Actual half-life ; Alpha-fetoprotein Malignant germ-cell tumor ; Hepatic malignancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a retrospective study, the prognostic value of monitoring the decay of alpha-fetoprotein (AFP) was assessed. Serum AFP was determined serially in 18 children with malignant germ-cell or hepatic tumors: 7 endodermal sinus tumor, 3 embryonal carcinoma, 5 malignant teratoma, 2 hepatoblastomas, and 1 hepatocellular carcinoma. The actual half-life (AHL) of AFP was computed after surgical resection of the tumor. In group 1, which had complete resection and no recurrence during follow-up (n = 13), the AHL of AFP was 4.0 ± 0.9 days. In group 2, which had incomplete resection or recurrence during follow-up (n = 5), the AHL of AFP was 24.8 ± 20 days, significantly longer than that of group 1 (P = 0.0026). The increased AHL of AFP indicated residual active tumor after surgical resection. The AHL of AFP may be more sensitive than serial monitoring of AFP in detecting preclinical recurrence after surgical resection of AFP-secreting tumors. Treatment strategies can be based on AFP clearance, and prospective clinical trials are warranted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01371908
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Alteration of signal-transducing molecules in tumor-infiltrating lymphocytes and peripheral blood T lymphocytes from human colorectal carcinoma patients (1998)
    Springer
    Cancer immunology immunotherapy 45 (1998), S. 299-305 
    Details
    ISSN: 1432-0851
    Keywords: Key words TCR-CD3 ; TIL ; Colorectal cancer ; Protein tyrosine kinase (PTK) ; Immune suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Tumor development or growth is accompanied by impaired immune responses, such as a poor proliferative response or down-regulated cytolytic T lymphocyte activity. Although recent reports have suggested that modification of the signal-transducing molecule is responsible for impaired immune responses in tumor-bearing hosts, the causes of defective immune function are not yet completely understood. Furthermore, the clinical significance of the findings is not yet clear. In this study, we investigated the alteration of several signal-transducing molecules in peripheral blood T lymphocytes (T-PBL) as well as in tumor-infiltrating lymphocytes (TIL) from human colorectal carcinoma patients and their relationship with the impaired host immune responses. A greater reduction in CD3ζ chain level was observed in TIL than in T-PBL from tumor-bearing hosts. CD3ζ chain reduction in T-PBL correlated with the clinicopathological stage of a tumor, especially with the status of lymph node metastasis. The levels of p56 lck and p59 fyn protein tyrosine kinase in T-PBL were also compared between tumor-bearing hosts and normal healthy volunteers. In T-PBL from tumor-bearing hosts, expression of protein tyrosine kinase p59 fyn was significantly lower than that of p56 lck . However, the level of CD3ζ chain expression did not correlate with T lymphocyte functions such as T lymphocyte proliferative response or allogeneic target cell lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050446
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