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  • 1
    Electronic Resource
    Electronic Resource
    Effect of platelet homogenate on in vitro glycosaminoglycans production by dermal fibroblasts from systemic sclerosis patients and normal controls (1985)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 113 (1985), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Confluent cultures of dermal fibroblasts from the involved skin of systemic sclerosis patients (SF) and a matched skin site of normal controls (NF) were investigated for the synthesis of glycosaminoglycans (GAG) in response to various concentrations of human platelet homogenate (PH). Experiments were carried out in the presence of 1% and 15% human serum (HS). In the absence of PH, GAG synthesis was higher in SF than NF. An increase in GAG synthesis was demonstrated in both SF and NF as the concentration of PH was increased to 200 μg/ml of growth medium. The PH-stimulated GAG synthesis occurred in 15% HS treated SF and NF, but there was no GAG synthesis increase in 1% HS-treated NF. The absolute count of GAG synthesis was always greater in SF than NF. The addition of PH in concentrations higher than 200μg/ml led to cell death of both SF and NF. These findings are the first to indicate a difference between SF and NF response to PH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1985.tb02070.x
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  • 2
    Electronic Resource
    Electronic Resource
    017
Laminin 10 in Wound Reepithelization and Angiogenesis (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Extracellular matrix laminins are the major noncollagenous components in the basement membrane zones (BMZ) and influence a variety of important biological processes including cell attachment, migration, and tissue development. A newly discovered laminin family member, laminin 10 (alpha 5, beta 1, and gamma 1), is found a major laminin present in the BMZs at the dermal-epidermal junction and dermal microvascular vessels. We examined the roles of laminin 10 in cutaneous wound repair in vitro and in vivo. Purified laminin 10 protein promoted human keratinocyte spreading and attachment, which is mediated by integrins. In human wound lesions, immunoperoxidase staining, with antibody 4C7 specific to laminin alpha 5 chain, stained strongly positive for laminin 10 in newly formed BMZs of epidermis and dermal blood vessels. Using a laminin 10 alpha 5 chain knockout mouse, excisional wounds in this model had abnormal reepithelialization and decreased granulation tissue formation. Transmission electron microscopy analysis of the BMZ of the mutant skin showed discontinuous lamina densa formation in areas between hemidesmosomes. In a porcine burn wound model, Reverse Transcription and Polymerase Chain Reaction analysis found significant higher expression of laminin 10 shortly after wounding and persisted through day 21, peaking at day 10. This expression pattern is consistent with the reconstitution of BMZs in the epidermis and microvascular blood vessels. These studies indicate that laminin 10 plays important roles in wound healing by promoting cell migration, BMZ assembly, reepithelialization, and angiogenesis.This study was supported by grants from NIH/NIAMES, DOD/DARPA and the Dermatology Foundation of South Florida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130215q.x
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  • 3
    Electronic Resource
    Electronic Resource
    055
The Effect of an Oxygen Emulsion on Collagen Deposition in Second-Degree Burn Wound Repair (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A porcine model of second-degree burn wound was used to evaluate the effect of a newly developed topical oxygen emulsion (TOE) on collagen deposition during wound repair. A total of 6 pigs were used for the study. The burn wounds were treated with air exposure (no treatment), vehicle control or TOE, which contains super-saturated oxygen and releases oxygen in sustained high level when applied topically. Skin wound samples were collected at days 0, 1, 4, 7, 10, 14 and 21 after wounding. Semi-quantitative Reverse Transcription and Polymerase Chain Reactions (RT-PCR) were used to examine the mRNA expressions for type I and type III collagens and matrix metalloprotease-1 (MMP-1). RT-PCR products were run on ethidium bromide gel and analyzed under UV light with Bio-Rad Gel Document 2000 system. The expression intensity was recorded as the mean gray value. One-way analysis of variance was used for statistical analysis. The results showed: 1). Higher mRNA expressions of collagen III and MMP-1 were observed in TOE treatment group compared with air exposure and vehicle control groups. 2). MMP-1 expression increased shortly after wounding with peak at days 4 and 7. 3). Collagen III expression increased earlier with peak at day 10 and day 14. 4). Collagen I expression level increased later than that of collagen III. Significant increase was seen after day 10 with the highest at day 21. However, there was no significant difference between vehicle control and TOE treatment groups. The data suggests that sustained high level of oxygen release by TOE may promote wound repair through the mechanism of increased expressions of type I and type III collagens. The TOE also increased the expression of MMP-1, which might accelerate clearing damaged collagens in the earlier phase of wound healing and promote collagen remodeling in the later phase.This study was supported by grants from DOD/DARPA and the Dermatology Foundation of South Florida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractbb.x
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  • 4
    Electronic Resource
    Electronic Resource
    090
Extracellular Matrix Regulation and Dermal Repair in Burn Wound (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To better understand the mechanisms of dermal restoration in burn wound we examined the mRNA expressions, using a porcine secondary-degree burn wound model, of major dermal matrices including type I, III collagens and elastin, basement membrane components of collagen IV and laminins, regulatory Matrix Metalloproteinase MMP 1 and MMP 9, as well as growth factors of bFGF, CTGF, TGFβ, and VEGF. Fresh skin burn wound samples from six pigs were collected at days 0, 1, 4, 7, 10, 14, and 21. Semiquantitative Reverse Transcription and Polymerase Chain Reactions (RT-PCR) were used with gene-specific primers. RT-PCR products were run on ethidium bromide gel and analyzed under UV light with Bio-Rad Gel Document 2000 system. One-way analysis of variance was used for statistical analysis. The results showed:'1) There were increases of all dermal extracellular matrices and growth factors tested; 2) A significant increase of type IV collagen occurred the earliest, while expressions of type I collagen and elastin increased at the latest; 3) A dramatic induction of MMP 1 and MMP 9 was observed shortly after wounding, which lasted through entire experiment; 4) The increased expression of VEGF, which is critical to angiogenesis, occurred early with peak at days 1 and 4, respectively. The expression pattern of bFGF, which has strong promoting effects on both endothelial cells and fibroblasts, is similar to that of VEGF; 5) For growth factors important for matrix deposition, both TGF beta 1 and 3 expressions increased gradually with sustained high level during entire experiment. The expression of CTGF, which is important in matrix production and remodeling, increased in later stage. This study showed the correlation between growth factor expression and dermal matrix deposition, which may have implications in developing strategies to improve burn wound healing.This study was supported by grants from DOD/DARPA and the Dermatology Foundation of South Florida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130215cl.x
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  • 5
    Electronic Resource
    Electronic Resource
    Skin grafts as pharmacological agents: pre-wounding of the donor site (1996)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 135 (1996), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary Initially thought to act as tissue replacement, cultured epithelial allografts arc now known to work by providing a potent stimultis for healing. In a similar fashion, we believe that traditional autografts may also provide a stimulus to help heal chronic wounds, thus acting as pharmacological agents for healing. We attempted to assess the possibility of augmenting the stimulatory properties of donor skin by initiating the healing process in the donor region prior to grafting. This was accomplished by pre-wounding the donor area 3 days prior to harvesting the donor skin. We compared these ‘pre-wounded’ grafts to those harvested immediately. Two patients underwent punch grafting for chronic leg ulceration. Half of the ulcer was grafted with donor skin harvested from an area that was pre-wounded and the other half from freshly harvested skin. We evaluated each for improvement of granulation tissue and degree of edge effect (migration ofthe previously dormant wound edges). All the grafts did well. There was marked improvement in granulation tissue in the ulcer hed after grafting, and the obvious presence of an edge effect. The edge eflect vvas increased on the site where the pre-wounded grafts were placed. It may be possible to augment the growth stimulatory properties of donor skin. This may offer therapeutic options in patients with chronic wounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1996.tb01164.x
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  • 6
    Electronic Resource
    Electronic Resource
    Support and stimulation of epidermal cell outgrowth from porcine skin explants by platelet factors (1986)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 115 (1986), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effect of a platelet homogenate fraction (PHF) on epidermal cell outgrowth from porcine skin explants was examined in culture medium with and without 5% foetal bovine serum (FBS). Explants were sustained and outgrowth was initiated, supported and stimulated by PHF, in the absence of FBS. PHF-supported outgrowth was concentration-dependent up to 130 μg protein/ml. PHF activity was not removed by heat treatment at 100° C for 2 min but was destroyed by heating at 100° C for 5 min. The factor responsible for epidermal cell outgrowth in PHF may be epibolin or serum spreading factor, or may be a platelet-derived factor as yet undescribed. Viability and outgrowth were stimulated by the ‘high molecular weight’ fraction of PHF (〉30 kd). PHF plus 5% FBS gave inhibition of outgrowth, which was not PHF concentration-dependent; however, this inhibitory effect was variable with different PHF preparations. This study is the first to demonstrate an effect of platelet-derived factors on epidermal cell viability and outgrowth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1986.tb05762.x
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