ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
A porcine model of second-degree burn wound was used to evaluate the effect of a newly developed topical oxygen emulsion (TOE) on collagen deposition during wound repair. A total of 6 pigs were used for the study. The burn wounds were treated with air exposure (no treatment), vehicle control or TOE, which contains super-saturated oxygen and releases oxygen in sustained high level when applied topically. Skin wound samples were collected at days 0, 1, 4, 7, 10, 14 and 21 after wounding. Semi-quantitative Reverse Transcription and Polymerase Chain Reactions (RT-PCR) were used to examine the mRNA expressions for type I and type III collagens and matrix metalloprotease-1 (MMP-1). RT-PCR products were run on ethidium bromide gel and analyzed under UV light with Bio-Rad Gel Document 2000 system. The expression intensity was recorded as the mean gray value. One-way analysis of variance was used for statistical analysis. The results showed: 1). Higher mRNA expressions of collagen III and MMP-1 were observed in TOE treatment group compared with air exposure and vehicle control groups. 2). MMP-1 expression increased shortly after wounding with peak at days 4 and 7. 3). Collagen III expression increased earlier with peak at day 10 and day 14. 4). Collagen I expression level increased later than that of collagen III. Significant increase was seen after day 10 with the highest at day 21. However, there was no significant difference between vehicle control and TOE treatment groups. The data suggests that sustained high level of oxygen release by TOE may promote wound repair through the mechanism of increased expressions of type I and type III collagens. The TOE also increased the expression of MMP-1, which might accelerate clearing damaged collagens in the earlier phase of wound healing and promote collagen remodeling in the later phase.This study was supported by grants from DOD/DARPA and the Dermatology Foundation of South Florida.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractbb.x
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