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  • 1
    Electronic Resource
    Electronic Resource
    055
The Effect of an Oxygen Emulsion on Collagen Deposition in Second-Degree Burn Wound Repair (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A porcine model of second-degree burn wound was used to evaluate the effect of a newly developed topical oxygen emulsion (TOE) on collagen deposition during wound repair. A total of 6 pigs were used for the study. The burn wounds were treated with air exposure (no treatment), vehicle control or TOE, which contains super-saturated oxygen and releases oxygen in sustained high level when applied topically. Skin wound samples were collected at days 0, 1, 4, 7, 10, 14 and 21 after wounding. Semi-quantitative Reverse Transcription and Polymerase Chain Reactions (RT-PCR) were used to examine the mRNA expressions for type I and type III collagens and matrix metalloprotease-1 (MMP-1). RT-PCR products were run on ethidium bromide gel and analyzed under UV light with Bio-Rad Gel Document 2000 system. The expression intensity was recorded as the mean gray value. One-way analysis of variance was used for statistical analysis. The results showed: 1). Higher mRNA expressions of collagen III and MMP-1 were observed in TOE treatment group compared with air exposure and vehicle control groups. 2). MMP-1 expression increased shortly after wounding with peak at days 4 and 7. 3). Collagen III expression increased earlier with peak at day 10 and day 14. 4). Collagen I expression level increased later than that of collagen III. Significant increase was seen after day 10 with the highest at day 21. However, there was no significant difference between vehicle control and TOE treatment groups. The data suggests that sustained high level of oxygen release by TOE may promote wound repair through the mechanism of increased expressions of type I and type III collagens. The TOE also increased the expression of MMP-1, which might accelerate clearing damaged collagens in the earlier phase of wound healing and promote collagen remodeling in the later phase.This study was supported by grants from DOD/DARPA and the Dermatology Foundation of South Florida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractbb.x
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  • 2
    Electronic Resource
    Electronic Resource
    090
Extracellular Matrix Regulation and Dermal Repair in Burn Wound (2005)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To better understand the mechanisms of dermal restoration in burn wound we examined the mRNA expressions, using a porcine secondary-degree burn wound model, of major dermal matrices including type I, III collagens and elastin, basement membrane components of collagen IV and laminins, regulatory Matrix Metalloproteinase MMP 1 and MMP 9, as well as growth factors of bFGF, CTGF, TGFβ, and VEGF. Fresh skin burn wound samples from six pigs were collected at days 0, 1, 4, 7, 10, 14, and 21. Semiquantitative Reverse Transcription and Polymerase Chain Reactions (RT-PCR) were used with gene-specific primers. RT-PCR products were run on ethidium bromide gel and analyzed under UV light with Bio-Rad Gel Document 2000 system. One-way analysis of variance was used for statistical analysis. The results showed:'1) There were increases of all dermal extracellular matrices and growth factors tested; 2) A significant increase of type IV collagen occurred the earliest, while expressions of type I collagen and elastin increased at the latest; 3) A dramatic induction of MMP 1 and MMP 9 was observed shortly after wounding, which lasted through entire experiment; 4) The increased expression of VEGF, which is critical to angiogenesis, occurred early with peak at days 1 and 4, respectively. The expression pattern of bFGF, which has strong promoting effects on both endothelial cells and fibroblasts, is similar to that of VEGF; 5) For growth factors important for matrix deposition, both TGF beta 1 and 3 expressions increased gradually with sustained high level during entire experiment. The expression of CTGF, which is important in matrix production and remodeling, increased in later stage. This study showed the correlation between growth factor expression and dermal matrix deposition, which may have implications in developing strategies to improve burn wound healing.This study was supported by grants from DOD/DARPA and the Dermatology Foundation of South Florida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130215cl.x
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  • 3
    Electronic Resource
    Electronic Resource
    Are excessive granulation tissue formation and retarded wound contraction due to decreased collagenase activity in wounds in tight-skin mice? (1994)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 131 (1994), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Wound contraction is delayed in tight-skin mice but the mechanism(s) are unknown. The purpose of this study was to investigate collagenase levels and the formation of granulation tissue in experimental wounds in tight-skin mice. One full-thickness skin excision (20×20 mm) was made on the back of nine tight-skin and eight normal mice. Granulation tissue analyses were performed 7 days post-operatively. The collagenase activity was determined by the use of a radiolabelled telopeptide-free collagen substrate, and the amount of granulation tissue was determined gravimetrically. Wound contraction was delayed (P〈0.001) in tight-skin mice (mean 22%) compared with normal mice (mean 46%). The collagenase activity was decreased (P〈0.05) by 40%, whereas the quantity of granulation tissue was increased (P〈0.001) by 60% in the wounds of tight-skin mice. Decreased collagenase content may provide one explanation for the delayed contraction of full- thickness wounds in tight-skin mice. Furthermore, this animal wound model may prove useful in the understanding of the pathogenesis, and in exploration of treatment, of excessive granulation tissue formation during wound healing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1994.tb08520.x
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  • 4
    Electronic Resource
    Electronic Resource
    In vivo 3-D distributions of electric fields in pig skin with rectangular pulse electrical current stimulation (RPECS) (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 17 (1996), S. 253-262 
    Details
    ISSN: 0197-8462
    Keywords: wound healing ; RPECS ; perpendicular field ; tangential field ; pig model ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: We developed stimulating and detecting electrodes. We experimentally examined three dimensional (3-D) distributions of electric fields in living pig skin under and around the stimulating electrodes with the detecting electrodes and rectangular pulsed electrical current stimulation (RPECS). We verified our previous physical assumption, E ≈ I / (A σdz), in the skin under the electrode, where E, I, A and σdz respectively represent the electric field, the externally imposed peak current, the cross sectional area of the stimulating electrode and the perpendicular conductivity of the skin. Pulses were 30 mA, 140 μs and 128 pulses per second (pps). These parameters were previously used in our laboratory to enhance cutaneous regeneration, in vivo, with RPECS. © 1996 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 5
    Electronic Resource
    Electronic Resource
    Conductivities of pig dermis and subcutaneous fat measured with rectangular pulse electrical current (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 17 (1996), S. 458-466 
    Details
    ISSN: 0197-8462
    Keywords: skin wound healing ; rectangular pulse electrical currents ; perpendicular ; tangential ; anisotropic ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: We examined experimentally the relationship between perpendicular and tangential electrical conductivities, σ, and peak current density J, in pig skin dermis and subcutaneous fat specimens by using a four-electrode measuring system with rectangular pulse electrical current (RPEC). We also investigated the relationship of the conductivity, σ, vs. pulse rate, f. The rates were selected at 8, 32, 64, and 128 pulses per second (pps), and the pulse width was fixed at 140 μs. These values are often used in vivo to enhance cutaneous regeneration with RPEC stimulation. It was found that the conductivities may be approximated to be $$\sigma_d= [\sigma_{dx}\sigma_{dy}\sigma_{dz}]= \hbox{[0.38 S/m 0.38 S/m 0.27 S/m]}$$ for the skin dermis and $$\sigma_f= [\sigma_{fx}\sigma_{fy}\sigma_{fz}]= \hbox{[0.074 S/m 0.074 S/m 0.045 S/m]}$$ for the subcutaneous fat in the conditions of this experiment. These findings implies that the conductivities of pig skin dermis and subcutaneous fat are anisotropic, i.e., σx = σy ≠ σz. It was also found that the conductivities are independent of current density and pulse rate in the current range from 20 μA/cm2 to 120 mA/cm2. © 1996 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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