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  • 1
    Electronic Resource
    Electronic Resource
    Bacterial phosphoenolpyruvate-dependent phosphotransferase system: association state of membrane-bound mannitol-specific enzyme II demonstrated by radiation inactivation (1987)
    s.l. : American Chemical Society
    Biochemistry 26 (1987), S. 6689-6696 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00395a019
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of high hydrostatic pressure on ‘passive’ monovalent cation transport in human red cells (1986)
    Springer
    The journal of membrane biology 94 (1986), S. 1-17 
    Details
    ISSN: 1432-1424
    Keywords: human red cell ; hydrostatic pressure ; ‘passive’ cation transport ; volume-sensitive KCl transport ; activation volume ; erythrocyte morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effects of high hydrostatic pressure (up to 400 ATA) on the ‘passive’ (defined as ouabain + bumetanide + EGTA-insensitive) influx and efflux of radiotracer cations (K+ Rb+, Na+, Cs+) has been studied in human red cells suspended at different medium tonicities giving altered cell volumes. Under all conditions studied, cation permeability was raised at pressure, and at least two distinct components were found to comprise this flux. Thus, increasing pressure (1) caused a generalized increase in cation permeability which was unaffected by the anion present, demonstrated linear concentration dependence, and wasreduced with cell swelling, and (2) stimulated a specific KCl pathway which was Cl− dependent, demonstrated saturation kinetics with raised [K]o and wasincreased with cell swelling. High hydrostatic pressure caused a significant alteration to red cell morphology from the normal biconcave disc to cup-shaped forms and it is proposed that this is associated with the unmasking of the volume-sensitive KCl system (2).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01901009
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  • 3
    Electronic Resource
    Electronic Resource
    Interaction of L antibody with low potassium-type sheep red cells: Resolution of two separate functional antibodies (1982)
    Springer
    The journal of membrane biology 64 (1982), S. 167-174 
    Details
    ISSN: 1432-1424
    Keywords: red cell membranes ; potassium transport ; alloimmune antiserum ; high K-low K polymorphism ; sheep red cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Antibodies of two specificities in alloimmune sheep anti-L sera, anti-L P and anti-L l , were separated by a new technique and characterized. Absorption of anti-L serum with trypsinized LK (LL) sheep red cells left anti-L P antibodies; the absorbed anti-L l antibodies were then eluted. Anti-L P was only weakly lytic in the presence of complement; it had no effect on passive K influx, but stimulated active K influx. The stimulation could be reversed by eluting the antibody in glycine buffer at low pH. Stimulatory activity in the eluted cells could be restored by resensitization with anti-L P . Anti-L l was more strongly lytic than anti-L P in the presence of complement; it had no effect on active K influx, but inhibited passive K influx. Pig anti-ruminant IgG conjugated to hemocyanin was used to visualize by electron microscopy the number of L P and L l antigen sites onLL sheep red cells sensitized with anti-L P and anti-L l . The values obtained were 590 L P sites/cell and 847 L l sites/cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01870882
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  • 4
    Electronic Resource
    Electronic Resource
    Thiocyanate transport across fish intestine (Pleuronectes platessa) (1982)
    Springer
    The journal of membrane biology 66 (1982), S. 9-14 
    Details
    ISSN: 1432-1424
    Keywords: thiocyanate ; chloride ; transport ; intestine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary When bathed on both sides with identical chloride-containing salines thein vitro preparation of the plaice intestine maintains a negative (serosa to mucosa) short-circuit current of 107±11 μA/cm2, a transepithelial potential difference of 5.5±0.6 mV (serosa negative), and a mean mucosal membrane potential of −45.4±0.6 mV. Under these conditions the intracellular chloride activity is 32mm. If chloride in the bathing media is partially, or completely substituted by thiocyanate the measured electrical parameters do not change but transepithelial flux determinations show a reduction in chloride fluxes and the presence of a significant thiocyanate flux. The addition of piretanide (10−4 m) reduced the short-circuit current and the mucosa-to-serosa fluxes of chloride and thiocyanate; this inhibition is similar to the effect of piretanide on chloride transport in this tissue. The results indicate that thiocyanate is transported in this tissue via the piretanide-sensitive “chloride” pathway and are compared with the effects of thiocyanate on other tissues reported in the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868477
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  • 5
    Electronic Resource
    Electronic Resource
    Pressure and temperature effects on human red cell cation transport (1982)
    Springer
    The journal of membrane biology 68 (1982), S. 47-56 
    Details
    ISSN: 1432-1424
    Keywords: hydrostatic pressure ; potassium flux ; erythrocyte membrane ; water of hydration ; anion effect ; thermodynamic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effects of hydrostatic pressure and temperature on the three components of K+ uptake in human red cells have been investigated, using ouabain and bumetanide to distinguish between the pump, passive diffusion and cotransport. The pressure sensitivity for passive diffusion has been shown to depend on the counter-ion present. The order of this effect, Cl−〉Br−〉NO 3 − 〉I−, is the same as for the ionic partial modal volumes and the Hofmeister series. We have analyzed our experimental results thermodynamically, and propose a model for the activated transition-state complex of the potassium ion which involves the loss of water molecules from the secondary hydration shell, cosphere II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872253
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  • 6
    Electronic Resource
    Electronic Resource
    Measurement and stoichiometry of bumetanide-sensitive (2Na∶1K∶3Cl) cotransport in ferret red cells (1985)
    Springer
    The journal of membrane biology 85 (1985), S. 205-213 
    Details
    ISSN: 1432-1424
    Keywords: sodium-potassium chloride cotransport ; red blood cells ; bumetanide ; stoichiometry ; ferret
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The bumetanide-sensitive uptake of Na+, K−(Rb−) and Cl− has been measured at 21°C in ferrent red cells treated with (SITS+DIDS) to minimize anion flux via capnophorin (Band 3). During the time course of the influx experiments tracer uptake was a first-order rate process. At normal levels of external Na+ (150mm) the bumetanide-sensitive uptake of K+ was dependent on Cl− and represented almost all of the K+ uptake, the residual flux demonstrating linear concentration dependence. The uptake of Na+ and Cl− was only partially inhibited by bumetanide indicating that pathways other than (Na+K+Cl) cotransport participate in these fluxes. The diuretic-sensitive uptake of Na+ or Cl− was, however, abolished by the removal of K+ or the complementary ion indicating that bumetanide-sensitive fluxes of Na+, K+ and Cl− are closely coupled. At very low levels of [Na] o (〈5mm) K+ influx demonstrated complex kinetics, and there was evidence of the unmasking of a bumetanide-sensitive Na+-independent K+ transport pathway. The stoichiometry of bumetanide-sensitive tracer uptake was 2Na∶1K∶3Cl both in cells suspended in a low and a high K+-containing medium. The bumetanide-sensitive flux was markedly reduced by ATP depletion. We conclude that a bumetanide-sensitive cotransport of (2Na∶1K∶3Cl) occurs as an electroneutral complex across the ferret red cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871515
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  • 7
    Electronic Resource
    Electronic Resource
    Interaction of alkyl ammonium derivatives with red cells: Hemolysis and sodium pump inhibition studies (1980)
    Springer
    The journal of membrane biology 55 (1980), S. 123-131 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Members of four homologous series of tetra-alkyl ammonium bromides (R 3N+(CH2) n−1·CH3Br− whereR=H, CH3 or C2H5 andR′N+H3Br− whereR′ represents the isomeric butyl series) have been synthesized and tested as sodium pump inhibitors, measured as ouabain-sensitive K+ influx, and as hemolytic agents on human red cells. Potency for both effects is presented graphically, plotting the logarithm of the concentration for half maximal effect against alkyl chain length. Both hemolysis and pump inhibition studies yielded a biphasic response consisting of two good straight lines, with effectiveness increasing up to C10–12 and then remaining constant up to C20. For hemolysis the alkyl ammonium series was most effective. The calculated free-energy change per methylene group was the same for three series of compounds, but the free-energy contribution from the headgroup was lower for the ammonium series. In contrast, although pump inhibition studies also yielded simple biphasic plots, inhibition occurred at 3- to 50-fold lower concentrations and there were significant differences between the three series, both in the free-energy changes per methylene group and in the headgroup contributions. We have analyzed these results thermodynamically to take account of hydrophobic interactions and the conformation of the alkyl chains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871154
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  • 8
    Electronic Resource
    Electronic Resource
    (Na+K)-ATPase activity in cattle red cells (1974)
    [s.l.] : Nature Publishing Group
    Nature 249 (1974), S. 864-864 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR,-Schatzmann1 has described a positive correlation between ouabain-sensitive ATPase activity and red cell K levels in cattle, cells with higher K levels giving a greater enzyme activity. These determinations were made with Na=150 mM, K=10mM. If ouabain-sensitive ATPase is determined as a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/249864a0
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  • 9
    Electronic Resource
    Electronic Resource
    Amino acid transport defect in glutathione-deficient sheep erythrocytes (1975)
    [s.l.] : Nature Publishing Group
    Nature 254 (1975), S. 156-157 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Sheep with low concentrations of red cell GSH were first found by Smith and Osburn2 and subsequent investigations have revealed at least two distinct types of congenital sheep red cell GSH deficiency3'5. The first is associated with a diminished activity of the first enzyme of GSH biosynthesis ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/254156a0
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  • 10
    Electronic Resource
    Electronic Resource
    Stimulation of the Potassium Transport System in Low Potassium Type Sheep Red Cells by a Specific Antigen Antibody Reaction (1969)
    [s.l.] : Nature Publishing Group
    Nature 222 (1969), S. 477-478 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Sheep of the genotype kafikafiMM were immunized with KaLKaLmm red cells and an antibody was produced (anti-m) which, after suitable absorptions, reacted with all LK type red cells, but not with HK type cells (Table 1). The titre was higher (1 in 32) against homozygous than against heterozygous LK ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/222477a0
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