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  • 1
    Electronic Resource
    Electronic Resource
    A combination of wheatflour, ethanol and food additives inducing FDEIA (2002)
    Oxford, UK : Munksgaard International Publishers
    Allergy 57 (2002), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2002.23836_12.x
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  • 2
    Electronic Resource
    Electronic Resource
    NMDA receptors influence the intracellular calcium concen-tration and the expression of differentiation markers in HaCaT cells (2004)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc
    Experimental dermatology 13 (2004), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ionotropic glutamate receptors (ligand-gated, ion-channel proteins) of the N-methyl-d-aspartate (NMDA) receptor type could enable a transmembranous calcium influx from the extracellular space. Though ionotropic glutamate receptors are predominantly neuronal receptors, they are also expressed in non-neuronal tissues like keratinocytes. Therefore, investigations were performed to study the function of NMDA receptors in HaCaT cells. The intracellular calcium concentration of HaCaT cells was studied under the influence of the selective receptor agonist NMDA and the selective NMDA antagonist MK-801. The proliferation of HaCaT cells was investigated using the crystal-violet method. Furthermore, the expression of Cytokeratin 10 and Filaggrin was examined in HaCaT cells after blocking NMDA receptors with MK-801. Using NMDA, there was a significant increase in the number of HaCaT cells showing elevated intracellular calcium concentration, at a dose between 25 µm and 1 mm (up to 84.6% of cells). The NMDA-associated calcium influx could be significantly suppressed by prior application of MK-801. There was no influence of NMDA on the proliferation of HaCaT cells. There was also no cytotoxic effect of NMDA (up to 1 mm). The expression of Cytokeratin 10 and Filaggrin could be suppressed by blocking NMDA receptors with MK-801. The investigations show that glutamate receptors of the NMDA-type play a role in the differentiation of HaCaT cells by regulating their intracellular calcium concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0906-6705.2004.0212at.x
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  • 3
    Electronic Resource
    Electronic Resource
    Antitumour necrosis factor-α antibodies (infliximab) in the treatment of a patient with toxic epidermal necrolysis (2002)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 146 (2002), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2002.46833.x
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  • 4
    Electronic Resource
    Electronic Resource
    Synthesis of prenylquinones in chloroplasts (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 64 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb01222.x
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  • 5
    Electronic Resource
    Electronic Resource
    Controlled secretion into the culture medium of a hybrid -glucanase by Acetobacter methanolicus mediated by the kil gene of Escherichia coli located on a Tn5 -derived transposon (1997)
    Springer
    Applied microbiology and biotechnology 47 (1997), S. 530-536 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A Tn5-based transposon bearing the kil gene (killing protein), mediating controlled export of periplasmic proteins into the culture medium, was constructed (Tn5-KIL3). This transposon contained the kil gene of the ColE1 plasmid under the growth-phase-dependent promoter of the fic gene (filamentation induced by cAMP) of Escherichia coli, an interposon located upstream of kil, a kanamycin/neomycin-resistance gene, a multiple cloning site and the mob site. The transposition of Tn5-KIL3 to Acetobacter methanolicus showed a moderate transposition frequency (10−5–10−6). By insertion of a Bacillus hybrid β-glucanase (bgl ) as a model protein into the transposon (Tn5-LF3) it was shown that the secretion function as well as the gene of the target protein had been transferred to and stably integrated into the chromosome of A. methanolicus, and that the transposition of Tn5-LF3 was non-specific. β-Glucanase was highly overexpressed and secreted into the medium during stationary phase. Total and extracellular production of β-glucanase varied depending on the integration site of the transposon. The viability of the bacterial cells was not affected, and cell lysis did not occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050968
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  • 6
    Electronic Resource
    Electronic Resource
    Extracellular production of a hybrid β-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors (1997)
    Springer
    Applied microbiology and biotechnology 47 (1997), S. 120-126 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cultivation conditions for the extracellular production of a hybrid β-glucanase from Bacillus were established by using Escherichiacoli JM109 carrying the plasmid pLF3. This plasmid contained a novel secretion system consisting of the kil gene (killing protein) of plasmid ColE1 under the stationary-phase promoter of either the fic or the bolA gene, an omega interposon (Prentki and Krisch 1984) located upstream of the promoters and a hybrid β-glucanase gene of Bacillus. When controlled by the fic promoter, the kil gene led to a higher total production of β-glucanase and a higher protein secretion than when it was under control of the bolA promoter. When the effect of different distances between the stationary-phase promoters and the kil gene was investigated, a shorter distance was generally found to result in a higher secretion. With a complex growth medium, the kinetics of extracellular production of the enzyme depended on several operating variables, such as the salt concentration (NaCl) and the oxygen supply, which were varied by changing the culture volume and the shaking speed. In defined media the secretion of β-glucanase into the medium was increased significantly by the addition of glycerol as a carbon source and by prolonged cultivation. The strain with the highest production and secretion yield of β-glucanase [E. coli JM109(pLF3)] was tested on the fermenter scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050899
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  • 7
    Electronic Resource
    Electronic Resource
    Stoffwechselprodukte von Mikroorganismen (1976)
    Springer
    Archives of microbiology 107 (1976), S. 249-256 
    Details
    ISSN: 1432-072X
    Keywords: Antibiotic derivatisation ; Yeast antibiotic ; Tryptanthrin ; Tryptophan metabolism ; Candia lipolytica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Candida lipolytica synthetisiert das Antibioticum Tryptanthrin aus 1 Mol Tryptophan und 1 Mol Anthranilsäure. Bei Verfütterung von Tryptophan und substituierter Anthranilsäure, bzw. substituiertem Tryptophan und Anthranilsäure, konnten die zu erwartenden Tryptanthrinderivate isoliert und identifiziert werden. Die Enzyme der Tryptanthrinbiosynthese wiesen in bezug auf diese Substrate, mit Ausnahme von Bromtryptophan, keine Spezifität auf. Parallel zu diesen Versuchen wurden durch chemische Synthese substituierte Tryptanthrine hergestellt. Die Dierivate wurden auf ihre antibiotische Wirksamkeit geprüft; als besonders wirksam erwiesen sich die halogenierten Verbindungen.
    Notes: Abstract Candida lipolytica synthesizes the antibiotic tryptanthrin from 1 mole tryptophan and 1 mole anthranilic acid. When feeding tryptophan and substituted anthranilic acids, or substituted tryptophans and anthranilic acid, we could isolate and identify the expected derivatives of tryptanthrin. The enzymes of the biosynthesis of tryptanthrin, with the exception of bromotryptophan, had no specifity for these substrates. In addition to these experiments substituted tryptanthrines were chemically synthesized. We checked them for antibiotic action; the halogen compounds turned out to be especially effective.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425335
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  • 8
    Electronic Resource
    Electronic Resource
    The kil gene of the ColE1 plasmid of Escherichia coli controlled by a growth-phase-dependent promoter mediates the secretion of a heterologous periplasmic protein during the stationary phase (1997)
    Springer
    Archives of microbiology 167 (1997), S. 143-150 
    Details
    ISSN: 1432-072X
    Keywords: Key words Protein secretion ; kil Gene ; β-Glucanase ; Escherichia coli ; Growth-phase-dependent promoter ; Stationary phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterologous gene products produced by Escherichia coli cells can be exported into the culture medium by the action of the kil gene of the ColE1 plasmid, which encodes a bacterial release protein. The kil gene was fused with the stationary-phase promoter of the fic gene of E. coli, and a secretion cassette (Kil-Km cassette) containing the regulated kil gene, the Km-resistance gene, and multiple cloning sites for the integration of target genes was constructed. Using the gene for β-glucanase (bgl) as a target gene, it was shown that the protein produced was only secreted into the medium during the stationary phase. Quasi-lysis and lethality were not observed. The primary effect of the induction of the kil gene was the overproduction of β-glucanase. The total amount produced per milliliter of bacterial culture was almost threefold higher than that of the corresponding Kil– control. The protein pattern of periplasm and culture medium was analyzed before and after induction of the kil gene expression, indicating that the release of periplasmic proteins is semiselective. This secretion system is the first to use a growth-phase-regulated promoter for the expression of the kil gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050427
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  • 9
    Electronic Resource
    Electronic Resource
    Eine direkte Titrationsmethode für Alkaloidsalze, Aminophenazon und Antihistamine einschließlich quartärer Basen (1952)
    Springer
    Naturwissenschaften 39 (1952), S. 556-556 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00593202
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  • 10
    Electronic Resource
    Electronic Resource
    Ist die WITs-Beurteilung der sagittalen Kieferrelation nach A. Jacobson ein brauchbares diagnostisches Hilfsmittel? (1981)
    Springer
    Journal of orofacial orthopedics 42 (1981), S. 64-70 
    Details
    ISSN: 1615-6714
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary This paper shows the variations produced when seven persons draw the points A and B and the occlusal plane. Two methods ofJacobson [3, 4] for fixing the occlusal plane are compared. SNA, SNB and ANB-angles show slight differences when being drawn by seven persons. The results of the two methods of fixing the occlusal plane show significant differences. Thus the “WITs” appraisal ofJacobson does not seem to be very helpful but the individualized ANB-angle ofPanagioditis andWitt [5] seems to be helpful in solving this problem.
    Abstract: Résumé Ce travail montre les variations observées sur les tracés de sept personnes qui avaient dessiné les points A et B et le plan occlusal. On compare aussi deux méthodes deJacobson [3, 4] servant à tracer le plan d'occlusion. On ne note qu'une petite différence entre les angles SNA, SNB et ANB. Par contre les résultats des deux méthodes deJacobson varient de manière significative. C'est pourquoi l'évaluation de «WITs» nous semble être douteuse. Dans cet ordre d'idées, nous renvoyons le lecteur au travail dePanagiotidis etWitt [5]: L'angle ANB individualisé.
    Notes: Zusammenfassung Die vorliegende Studie zeigt die Variationen auf, denen die Punkte A und B sowie die Okklusionsebene bei der Festlegung durch sieben Durchzeichner unterliegen. Zudem werden zwei der vonJacobson [3, 4] angegebenen Methoden zur Festlegung der Okklusionsebene verglichen. Die Winkel SNA, SNB und ANB differieren bei den sieben Durchzeichnern wenig. Signifikant unterscheiden sich die Ergebnisse, die durch die beiden Methoden zur Festlegung der Okklusionsebene gefunden wurden. Die WITs-Beurteilung erscheint aus diesem Grunde fragwürdig. In diesem Zusammenhang wird auf den individualisierten ANB-Winkel nachPanagioditis u.Witt [5] verwiesen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02163465
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