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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural study of deep supercooling of xylem ray parenchyma cells from Styrax obassia
    Amsterdam : Elsevier
    Micron 25 (1994), S. 241-252 
    Details
    ISSN: 0968-4328
    Keywords: Styrax obassia ; cryo-scanning electron microscopy (cryo-SEM) ; deep supercooling ; freeze-fracture replica ; intracellular freezing ; xylem ray parenchyma cells
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0968-4328(94)90029-9
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  • 2
    Electronic Resource
    Electronic Resource
    Rabeprazole, amoxycillin and low- or high-dose clarithromycin for cure of Helicobacter pylori infection (2000)
    Oxford UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 14 (2000), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Rabeprazole sodium is a proton pump inhibitor.〈section xml:id="abs1-2"〉〈title type="main"〉Aim:To evaluate the efficacy and safety of 1-week triple therapy with rabeprazole, amoxycillin and clarithromycin for the eradication of Helicobacter pylori.〈section xml:id="abs1-3"〉〈title type="main"〉Methods:A total of 100 subjects with H. pylori were randomly divided into two groups of 1-week triple therapy with rabeprazole 10 mg b.d., amoxycillin 750 mg b.d. and either clarithromycin 200 mg b.d. (RAC400, n=50) or clarithromycin 400 mg b.d. (RAC800, n=50). Endoscopic examination with four biopsies (two specimens from the antrum and two from the gastric body) was performed. The status of H. pylori infection was determined using culture and histology (Giemsa stain) of the biopsy specimens. Sensitivity to clarithromycin was determined using the E-test: MIC 〉 8 g/mL was considered to be resistant, whereas MIC 〈 2 g/mL was considered to be sensitive. Cure was defined as no evidence of H. pylori infection 1 month after completion of treatment.〈section xml:id="abs1-4"〉〈title type="main"〉Results:There were no significant differences in the clinical characteristics of the two groups. Eradication rates (intention-to-treat and per protocol, respectively) were: RAC400: 86% (95% CI: 76–95%) and 89% (95% CI: 80–97%); RAC800: 94% (95% CI: 87–100%) and 97% (95% CI: 94–100%). There was no significant difference between the eradication rates of either regimen. Three subjects with failed eradication in the RAC400 group were all infected with a clarithromycin-resistant strain before beginning the therapy. Haemorrhagic colitis was the only severe adverse event, which was observed in one patient in the RAC800 group.〈section xml:id="abs1-5"〉〈title type="main"〉Conclusion:One-week triple therapy with rabeprazole, amoxycillin and low-dose clarithromycin is effective for the eradication of H. pylori infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.2000.00810.x
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  • 3
    Electronic Resource
    Electronic Resource
    Dynamic changes in the arrangement of cortical microtubules in conifer tracheids during differentiation. (1995)
    Springer
    Planta 197 (1995), S. 418-421 
    Details
    ISSN: 1432-2048
    Keywords: Cortical microtubule ; Cellulose microfibril ; Abies ; Tracheid ; Confocal laser scanning microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The arrangement of cortical microtubules (MTs) in differentiating tracheids of Abies sachalinensis Masters was examined by confocal laser scanning microscopy after immunofluorescent staining. The arrays of MTs in the tracheids during formation of the primary wall were not well ordered and the predominant orientation changed from longitudinal to transverse. During formation of the secondary wall, the arrays of MTs were well ordered and their orientation changed progressively from a flat S-helix to a steep Z-helix and then to a flat S-helix as the differentiation of tracheids proceeded. The orientation of cellulose microfibrils (MFs) on the innermost surface of cell walls changed in a similar manner to that of the MTs. These results provide strong evidence for the co-alignment of MTs and MFs during the formation of the semi-helicoidal texture of the cell wall in conifer tracheids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202666
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  • 4
    Electronic Resource
    Electronic Resource
    An immunohistochemical and electrophysiological study on Isk protein in the stria vascularis of the guinea pig (1993)
    Springer
    European archives of oto-rhino-laryngology and head & neck 250 (1993), S. 186-189 
    Details
    ISSN: 1434-4726
    Keywords: Isk protein ; K+ channels ; Stria vascularis ; Marginal cells ; Clofilium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The reaction product against rat Isk protein antibody was demonstrated on the endolymphatic surface of the marginal cell of the guinea pig, using an immunohistochemical method. Clofilium, a potassium channel blocker which has been previously found to block rat kidney Isk channels, decreased the endocochlear potential (EP) when applied by perilymphatic perfusion and iontophoresis into endolymph. This finding of a decreased EP supports the presence of Isk protein on the endolymphatic surface of the marginal cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171709
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  • 5
    Electronic Resource
    Electronic Resource
    Orientation of microfibrils and microtubules in developing tension-wood fibres of Japanese ash (Fraxinus mandshurica var. japonica) (1995)
    Springer
    Planta 196 (1995), S. 577-585 
    Details
    ISSN: 1432-2048
    Keywords: Cellulose microfibril ; Cortical microtubule ; Fraxinus ; Gelatinous layer ; Tension-wood fibre
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The orientation of cellulose microfibrils (MFs) and the arrangement of cortical microtubules (MTs) in the developing tension-wood fibres of Japanese ash (Fraxinus mandshurica Rupr. var. japonica Maxim.) trees were investigated by electron and immunofluorescence microscopy. The MFs were deposited at an angle of about 45° to the longitudinal axis of the fibre in an S-helical orientation at the initiation of secondary wall thickening. The MFs changed their orientation progressively, with clockwise rotation (viewed from the lumen side), from the S-helix until they were oriented approximately parallel to the fibre axis. This configuration can be considered as a semihelicoidal pattern. With arresting of rotation, a thick gelatinous (G-) layer was developed as a result of the repeated deposition of parallel MFs with a consistent texture. Two types of gelatinous fibre were identified on the basis of the orientation of MFs at the later stage of G-layer deposition. Microfibrils of type 1 were oriented parallel to the fibre axis; MFs of type 2 were laid down with counterclockwise rotation. The counterclockwise rotation of MFs was associated with a variation in the angle of MFs with respect to the fibre axis that ranged from 5° to 25° with a Z-helical orientation among the fibres. The MFs showed a high degree of parallelism at all stages of deposition during G-layer formation. No MFs with an S-helical orientation were observed in the G-layer. Based on these results, a model for the orientation and deposition of MFs in the secondary wall of tension-wood fibres with an S1 + G type of wall organization is proposed. The MT arrays changed progressively, with clockwise rotation (viewed from the lumen side), from an angle of about 35–40° in a Z-helical orientation to an angle of approximately 0° (parallel) to the fibre axis during G-layer formation. The parallelism between MTs and MFs was evident. The density of MTs in the developing tension-wood fibres during formation of the G-layer was about 17–18 per μm of wall. It appears that MTs with a high density play a significant role in regulating the orientation of nascent MFs in the secondary walls of wood fibres. It also appears that the high degree of parallelism among MFs is closely related to the parallelism of MTs that are present at a high density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203659
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  • 6
    Electronic Resource
    Electronic Resource
    Comparative studies on lignin distribution by UV microscopy and bromination combined with EDXA (1982)
    Springer
    Wood science and technology 16 (1982), S. 269-277 
    Details
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary In order to elucidate a previously reported discrepancy in the ratio of the lignin concentration in the middle lamella to that in the secondary wall as determined by ultraviolet (UV) microscopy and bromination combined with EDXA, the ultraviolet absorptivity of the lignin and the lignin reactivity towards bromination were compared for black spruce wood (Picea mariana Mill.). In addition, UV microscopy and EDXA techniques were applied to the determination of lignin distribution in the tracheids in order to establish the relationship between the two techniques. The results indicated that, although the ultraviolet absorptivity in different morphological regions is essentially the same, the secondary wall lignin was 1.70 times more reactive towards bromination than the middle lamella lignin. By applying the value of 1.70 as a correction to the EDXA results, the estimated lignin distribution by EDXA was in fairly good agreement with that from UV microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353151
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  • 7
    Electronic Resource
    Electronic Resource
    Relationship between ball milling and the susceptibility of wood to digestion by cellulase (1982)
    Springer
    Wood science and technology 16 (1982), S. 279-285 
    Details
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary Aspen wood was ball milled for times as long as 10 days. The change in wood morphology was examined in the ultraviolet microscope. The accessibility of the milled wood to cellulase was also determined. Soon after the commencement of the milling, part of the wood took on a characteristic appearance in which the cell wall material was completely broken down. The proportion of this disordered wall material (DWM) increased with increased time of milling. The accessibility to cellulase increased with the proportion of DWM produced by the milling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353153
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  • 8
    Electronic Resource
    Electronic Resource
    Quantitative analysis of lignin using an UV microscopic image analyser. variation within one growth increment (1981)
    Springer
    Wood science and technology 15 (1981), S. 45-55 
    Details
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary The variation of the lignin content within one growth increment of Abies sachalinensis was investigated with the aid of an ultraviolet microscopic image analyser. The lignin content was determined continuously in each cell within a growth increment. The direct photometric scanning method of UV image is believed to give accurate results for determination of the lignin content of the cell wall, since it contains fewer assumptions. The lignin content of the earlywood was higher than that of latewood in adult wood as same as the others. It was high, however, in the terminal zone of the latewood. The trends of the juvenile wood were quite different from those of the adult wood. The lignin content increased from earlywood to latewood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00366500
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