Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Molecular mimicry in diabetes mellitus: the homologous domain in coxsackie B virus protein 2C and islet autoantigen GAD65 is highly conserved in the coxsackie B-like enteroviruses and binds to the diabetes associated HLA-DR3 molecule (1998)
    Springer
    Diabetologia 41 (1998), S. 40-46 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Coxsackie B virus ; peptide binding ; HLA-DR ; molecular mimicry ; IDDM.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It has been proposed that molecular mimicry between protein 2C (p2C) of coxsackie virus B4 and the autoantigen glutamic acid decarboxylase (GAD65) plays a role in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). In this study we show that the amino acid sequence of p2C which shares homology with a sequence in GAD65 (PEVKEK), is highly conserved in coxsackie virus B4 isolates as well as in different viruses of the subgroup of coxsackie B-like enteroviruses. These are the most prevalent enteroviruses and therefore exposure to the mimicry motif will be a frequent event throughout life. Presentation of the homologous peptides by HLA molecules is essential for T-cell reactivity. Therefore, we tested whether the PEVKEK motif can bind to the IDDM-associated HLA-DR1, -DR3 and -DR4 molecules. Synthetic peptides with sequences derived from p2C and GAD65 did bind to HLA-DR3 but not to HLA-DR1 or -DR4. Replacement of amino acids within the motif showed that the PEVKEK motif binds specifically to HLA-DR3. Moreover, both p2C and GAD65 peptides bind in the same position within the peptide binding groove of the DR3 molecule which is an essential requirement for T-cell cross-reactivity. The results support molecular mimicry between p2C of coxsackie B-like enteroviruses and GAD65. However, this molecular mimicry may be limited to the HLA-DR3 positive subpopulation of IDDM patients. [Diabetologia (1998) 41: 40–46]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050864
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Respiratory syncytial virus pneumonia in an AIDS patient (1996)
    Springer
    Infection 24 (1996), S. 375-377 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir berichten über einen AIDS-Patienten, der eine Respiratory Syncytial Virus Pneumonie entwickelte. Zugleich bestand eine Infektion mit Cytomegalovirus. Der Patient sprach auf Ribavirin und Foscarnet gut an. Im Anschluß bestand bei dem Patienten, dessen schwere obstruktive Lungenkrankheit bekannt war, noch eine bronchiale Hyperreaktivit:at. Die Probleme der antiviralen Therapie werden diskutiert.
    Notes: Summary Pneumonia caused by respiratory syncytial virus in an AIDS patient is reported. A co-infection with cytomegalovirus was also demonstrated. Treatment with ribavirin and foscarnet produced good clinical response. The patient, known to have serious obstructive lung disease, suffered from bronchial hyperreactivity for some time afterwards. The dilemma of antiviral therapy is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01716083
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    16S rRNA based polymerase chain reaction compared with culture and serological methods for diagnosis ofMycoplasma pneumoniae infection (1994)
    Springer
    European journal of clinical microbiology & infectious diseases 13 (1994), S. 401-405 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The use of a 16S rRNA based polymerase chain reaction (PCR) for the detection ofMycoplasma pneumoniae infection was investigated. Sputum samples from 34 patients with respiratory illness and evidence of pneumonia as judged by chest X-ray were analyzed by PCR and microbiological culture. Throat swabs from 14 healthy individuals were used as controls. For serology, an enzyme immunoassay for the detection of immunoglobulin M antibodies and a complement fixation assay were performed. Evidence ofMycoplasma pneumoniae infection was obtained in ten patients (29 %), eight of whom were found positive by both PCR and serology. Two of the sputum samples from these eight patients were negative by culture. Of the remaining two patients positive forMycoplasma pneumoniae, one was positive by PCR and culture but negative by serology, and one was found positive by serology but negative by PCR and culture. Thirteen of the 14 controls were negative by both PCR and serology. One control, however, was negative by serology but positive by PCR, which was probably due to asymptomatic carriage ofMycoplasma pneumoniae. The results of this study indicate the suitability of the PCR for the detection ofMycoplasma pneumoniae in clinical samples as well as its potential value as an additional tool for the diagnosis of infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01971997
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Rapidly fatal Q-fever pneumonia in a patient with chronic granulomatous disease (1992)
    Springer
    Infection 20 (1992), S. 287-289 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Akutes Q-Fieber ist eine systemische Krankheit, die nur selten tödlich endet — etwa bei chronisch Kranken oder bei Auftreten einer Endokarditis. Im vorliegenden Fall führte eine akute Q-Fieber-Pneumonie, die bei einem 11jährigen Kind mit chronischer Granulomatose auftrat, zum Tod. Die Komplementbindungsreaktion zeigte einen Antikörpertiteranstieg auf 1:1,024. IgM war im Immunofluoreszenztest nachzuweisen. Der Erreger wurde in histologischen Schnitten in der Lunge nach Giemsa-Färbung und mittels indirekter Immunfluoreszenz nachgewiesen. Das Kind hatte sich wahrscheinlich während eines Urlaubs in Frankreich mitCoxiella burnetii infiziert. Die Behandlung erfolgte mit einer Vielzahl antimikrobieller Substanzen mit breitem Wirkungsspektrum, Substanzen mit Aktivität gegen Q-Fieber wie Chloramphenicol oder Tetrazykline wurden jedoch nicht eingesetzt.
    Notes: Summary Acute Q-fever is a systemic illness which rarely has a fatal outcome. Fatal cases do occur with the chronic form of the disease and associated with endocarditis. This report presents the case of a fatal, acute Q-fever pneumonia in an 11-year-old patient with chronic granulomatous disease. Complement fixation antibody titer rose to 1:1,024 with positive IgM in immunofluorescence. Giemsa stained lung sections and indirect immunofluorescence demonstrated the microorganisms in the tissues. TheCoxiella burnetii infection was probably contracted during a holiday trip to rural France. Despite the fact that the patient received a variety of antimicrobial agents with broad spectrum activity against bacteria and fungi, coverage for Q-fever, i.e. chloramphenicol or tetracyclines, was not included.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01710798
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...